Materials for cell encapsulation via a new tandem approach combining reverse thermal gelation and covalent crosslinking

Cellesi, Francesco; Tirelli, Nicola; Hubbell, Jeffrey A.

doi:10.1002/1521-3935(200207)203:10/11<1466::aid-macp1466>3.0.co;2-p

Cited by 89 publications

(103 citation statements)

References 32 publications

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“…The need for more stable hydrogels was identified also by Cellesi et al [67][68][69]. Their approach mimicked the natural thermal gelation of alginate by relying on the occurrence of a physical mechanism, attributed by the thermosensitive nature of Pluronics®, followed by an irreversible chemical mechanism, due to covalent crosslinking by the reaction of groups at the termini of the copolymer.…”

Section: Peo/ppo-based Systemsmentioning

confidence: 99%

“…It was found that these polymers were biocompatible, and so was their gelation process, which can be performed at physiological temperature and pH, allowing for encapsulation of sensitive drugs and cells [67]. In order to limit steric hindrance phenomena, a similar method was followed with Tetronic® polymers, which are thermosensitive tetra-armed Pluronic® analogues [68].…”

Section: Peo/ppo-based Systemsmentioning

confidence: 99%

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Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,135

696

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Environmentally responsive hydrogels have the ability to turn from solution to gel when a specific stimulus is applied. Thermoresponsive hydrogels utilize temperature change as the trigger that determines their gelling behavior without any additional external factor. These hydrogels have been interesting for biomedical uses as they can swell in situ under physiological conditions and provide the advantage of convenient administration. The scope of this paper is to review the aqueous polymer solutions that exhibit transition to gel upon temperature change. Typically, aqueous solutions of hydrogels used in biomedical applications are liquid at ambient temperature and gel at physiological temperature. The review focuses mainly on hydrogels based on natural polymers, N-isopropylacrylamide polymers, poly(ethylene oxide)-b-poly(propylene oxide)-bpoly(ethylene oxide) polymers as well as poly(ethylene glycol)-biodegradable polyester copolymers.

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Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Section: Peo/ppo-based Systemsmentioning

confidence: 99%

Thermoresponsive hydrogels in biomedical applications

Klouda

Mikos

2008

European Journal of Pharmaceutics and Biopharmaceutics

1,135

696

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“…Given that PEG is highly hydrophilic and generally nonadhesive to proteins or cells, it has found widespread use as a drug carrier [14], and many PEG hydrogels have been produced from aqueous solutions containing linear or branched PEG macromolecules via chemical crosslinking [15][16][17][18]. In addition to hydrogels formed via radical crosslinking reactions, PEG hydrogels have been formed, for example, via Michael-type addition reactions upon mixing with thiol-bearing compounds [19,20] or via the reaction between amino-terminated poly (ethylene glycol) and the herbal iridoid glycoside genipin [21]. In some cases, cell adhesive peptide domains or biodegradable sequences have been introduced into PEG hydrogels to endow them with biological signaling functions [22], including the capacity for growth factor delivery.…”

Section: Introductionmentioning

confidence: 99%

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Zhang

Furst

Kiick

2006

Journal of Controlled Release

117

131

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The design of materials in which assembly, mechanical response, and biological properties are controlled by protein-polysaccharide interactions could provide materials that mimic the biological environment and find use in the delivery of growth factors. In the investigations reported here, a heparin-binding, coiled-coil peptide, PF4 ZIP , was employed to mediate the assembly of heparinized polymers. The heparin-binding affinity of this peptide was compared with that of other heparinbinding peptides (HBP) via heparin-sepharose chromatography and surface plasmon resonance (SPR) experiments. Results from these experiments indicate that PF4 ZIP demonstrates a higher heparin-binding affinity and heparin association rate when compared to the heparin-binding domains of antithrombin III (ATIII) and heparin-interacting protein (HIP). Viscoelastic hydrogels were formed upon the association of PF4 ZIP -functionalized star poly(ethylene glycol) (PEG-PF4 ZIP ) with low-molecular-weight heparin-functionalized star PEG (PEG-LMWH). The viscoelastic properties of the hydrogels can be altered via variations in the ratio of LMWH to PF4 ZIP . bFGF release from these gels have also been investigated. Comparison of the bFGF release profiles with the hydrogel erosion profiles indicates that bFGF delivery from this class of hydrogels is mainly an erosioncontrolled process and the rates of bFGF release can be modulated via choice of HBP or via variations in the mechanical properties of the hydrogels. Manipulation of hydrogel physical properties and erosion profiles will provide novel materials for controlled growth factor delivery and other biomedical applications.

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“…In response to this demand, much research has focused on developing new biomaterials or improving existing systems. [1][2][3][4][5][6][7][8][9][10][11][12] While the design criteria driving this research cover as broad a range of material properties as diverse as the array of end-applications, they also share some common goals. Included among these goals is the desire to create biocompatible materials that have tunable material properties which allow optimal tissue regeneration and controllable drug delivery.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of degradable thiol-allyl ether photopolymers

Rydholm

Reddy

Anseth

et al. 2007

Polymer

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Degradable thiol-ene photopolymer networks were formed through radically mediated step growth reactions. Variations in the network structure were used to alter the initial and temporal moduli, mass loss profiles, and equilibrium swelling ratios. Mass loss rates varied with changes in the solvent concentration, monomer molecular weight, average monomer functionality, and concentration of degradable linkages. The time required for the networks to degrade completely ranged from 1.20 ± 0.01 to 24.5 ± 0.1 days, which corresponded to hydrolysis rates of 0.18 ± 0.01 and 0.021 ± 0.0003 days -1 . Initial moduli also varied considerably as a function of network structure, ranging from 150 ± 35 to nearly 5000 ± 100 kPa, and initial equilibrium swelling ratios ranged from 2.5 ± 0.01 to 18.7 ± 2. Collectively, these results demonstrate how the material properties and the mass loss behavior of thiol-ene networks can be independently tuned for specific applications.

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Materials for cell encapsulation via a new tandem approach combining reverse thermal gelation and covalent crosslinking

Cited by 89 publications

References 32 publications

Thermoresponsive hydrogels in biomedical applications

Thermoresponsive hydrogels in biomedical applications

Manipulation of hydrogel assembly and growth factor delivery via the use of peptide–polysaccharide interactions

Development and characterization of degradable thiol-allyl ether photopolymers

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