Materials science perspective of multifunctional materials derived from collagen

Ashokkumar, Meiyazhagan; Ajayan, Pulickel M.

doi:10.1080/09506608.2020.1750807

Cited by 25 publications

(13 citation statements)

References 261 publications

(364 reference statements)

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“…The use of gold nanoparticles (Au NPs) or carbon nanotubes (CNTs) to reinforce collagen hydrogels by increasing their mechanical properties is a strategy that has been attempted before. 21,22 Homenick et al 23 successfully prepared cast collagen hydrogels with single-walled carbon nanotubes (SWCNTs) by functionalizing the surface of CNTs with poly(ethyleneimine) (PEI), resulting in a nine-fold increase in Young's modulus compared to non-cross-linked hydrogels. Similarly, Kim et al 24 increased the stiffness of collagen fibers by introducing SWCNTs in the collagen matrix to study stem cell differentiation and Xing et al 25 prepared an injectable Au NPs-collagen hydrogel with improved mechanical properties.…”

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aligned and Oriented Collagen Nanocomposite Fibers as Substrates to Activate Fibroblasts

Spiaggia

Taladriz‐Blanco

Septiadi

et al. 2021

ACS Appl. Bio Mater.

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Purified collagen possesses weak mechanical properties, hindering its broad application in tissue engineering. Strategies based on manipulating the hydrogel to induce fiber formation or incorporate nanomaterials have been proposed to overcome this issue. Herein, we use a microfluidic device to fabricate, for the first time, collagen hydrogels with aligned and oriented fibers doped with gold nanoparticles and carbon nanotubes. Results based on rheology, atomic force microscopy, and scanning electron microscopy reveal the formation of aligned and oriented collagen fibers possessing greater rigidity and stiffness on the doped hydrogels in comparison with native collagen. The mechanical properties of the hydrogels increased with the nanomaterial loading percentage and the stiffest formulations were those prepared in the presence of carbon nanotubes. We further evaluate the in vitro response of NIH-3T3 fibroblasts to the change in stiffness. The cells were found to be viable on all substrates with directional cell growth observed for the carbon nanotube-doped collagen fibers. No significant differences in the cell area, aspect ratio, and intensification of focal adhesions driven by the increase in stiffness were noted. Nonetheless, fibroblast proliferation and secretion of TGF-β1 were greater on the hydrogels doped with carbon nanotubes. This nanomaterial-collagen composite provides unique features for cell and tissue substrate applications.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aligned and Oriented Collagen Nanocomposite Fibers as Substrates to Activate Fibroblasts

Spiaggia

Taladriz‐Blanco

Septiadi

et al. 2021

ACS Appl. Bio Mater.

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“…[ 36 ] The enriched collagen in SIS is organized with a hierarchical structure from the sub‐nanometer scale of amino acids to the micrometer scale of the fiber. [ 37,38 ] Collagen fibers in SIS are demonstrated with two dominant orientations aligned approximately ±30° from the longitudinal direction (Figure 1A‐ii,iii). [ 36 ] The van der Waals layer‐by‐layer structure of collagen fiber assembly in SIS is observed in the cross‐sectional scanning electron microscopy (SEM) image (Figure S1, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Van der Waals Exfoliation Processed Biopiezoelectric Submucosa Ultrathin Films

et al. 2022

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biocompatible, and biodegradable. However, inorganic piezoelectric materials are unsuitable for biological applications due to their inherent rigidity, fragility, and possible toxicity. Even those flexible piezoelectric organic polymers, for example, polyvinylidene difluoride (PVDF), are hard to meet many demands such as resorbability or degradability. [4] Piezoelectric biomaterials are promising alternatives since they naturally exhibit biocompatibility, reliability, and environmental sustainability. At present, the research on piezoelectric biomaterials is mainly focused on two types: piezoelectric biomolecules such as amino acids, peptides, virus, and cellulose, [5][6][7][8][9][10][11] and piezoelectric biological tissues like bone, wool, tendon, invertebrate exoskeletons, and epidermis. [12][13][14][15][16] However, due to the high cost and complexity of assembling and domain aligning the small biomolecules at a large scale, most of the research on their biological piezoelectricity is still at the theoretical level. [17] Additionally, because of the disorder of domains and the lack of ferroelectricity, biological tissues hardly exhibit piezoelectric properties at the macroscopic level, which limits the detection and application of their piezoelectricity. [18,19] Small intestinal submucosa (SIS) is the middle layer of small intestine, that supports the mucosa and joins it to the muscular layer. This material is one of the most extensively investigated scaffolds in both experimental and preclinical models for tissue repairing. SIS serves as a supporting scaffold to reconstruct various tissues such as tendon, arteriovenous tissues, and abdominal wall. [20,21] It has a great potential for versatile biomedical applications thanks to its biocompatibility and having no adverse response in cross-species transplantations. [22] In 1968, Fukada observed the direct piezoelectric effect in the intestines at the macroscale level. [23] However, the experimental quantitative determination of the intrinsic piezoelectric effect of SIS and the origin of its biological piezoelectricity have yet to be demonstrated due to its weak piezoelectricity at the macroscopic level and the limitation of measurement techniques. Although piezoresponse force microscopy (PFM) has been a powerful technique that allows quantitatively determining the piezoelectricity of biomaterials, [24,25] the thickness of the sample has a significant impact on the effective piezoresponses because the depth resolution of PFM is limited. [26,27] In addition, biological tissues hardly exhibit piezoelectricity at the thicker macroscopic level Piezoelectric biomaterials have attracted significant attention due to the potential effect of piezoelectricity on biological tissues and their versatile applications. However, the high cost and complexity of assembling and domain aligning biomolecules at a large scale, and the disordered arrangement of piezoelectric domains as well as the lack of ferroelectricity in natural biological tissues remain a roadblock toward practic...

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“…The chemical functionalization of collagen throughout the extraction process removes its natural bonding; thus, biomaterials containing collagen require inter-and intra-molecular cross-linking, with the goal of improving their mechanical characteristics [149,150]. In particular, cross-linkers such as glutaraldehyde [151], gamma radiation [152], N-hydroxy succinimide [153], carbodiimide [154], formaldehyde [155], hexamethylene diisocyanate [156], sodium tripolyphosphate [157], genipin [158], transglutaminase [159], dialdehyde [160], and sugar-based [161] ones, are utilized to augment the collagen hydrogels' stability that is necessary for preventing the quick enzymatic degradation [162].…”

Section: Collagen-based Hydrogelsmentioning

confidence: 99%

Protein-Based Hydrogels: Promising Materials for Tissue Engineering

et al. 2022

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The successful design of a hydrogel for tissue engineering requires a profound understanding of its constituents’ structural and molecular properties, as well as the proper selection of components. If the engineered processes are in line with the procedures that natural materials undergo to achieve the best network structure necessary for the formation of the hydrogel with desired properties, the failure rate of tissue engineering projects will be significantly reduced. In this review, we examine the behavior of proteins as an essential and effective component of hydrogels, and describe the factors that can enhance the protein-based hydrogels’ structure. Furthermore, we outline the fabrication route of protein-based hydrogels from protein microstructure and the selection of appropriate materials according to recent research to growth factors, crucial members of the protein family, and their delivery approaches. Finally, the unmet needs and current challenges in developing the ideal biomaterials for protein-based hydrogels are discussed, and emerging strategies in this area are highlighted.

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Materials science perspective of multifunctional materials derived from collagen

Cited by 25 publications

References 261 publications

Aligned and Oriented Collagen Nanocomposite Fibers as Substrates to Activate Fibroblasts

Aligned and Oriented Collagen Nanocomposite Fibers as Substrates to Activate Fibroblasts

Van der Waals Exfoliation Processed Biopiezoelectric Submucosa Ultrathin Films

Protein-Based Hydrogels: Promising Materials for Tissue Engineering

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