Maternal alloxan exposure induces damage in rat offspring lumbar vertebrae and protective role of arachidonic acid

Abstract

Background: Vertebral abnormalities in offspring of diabetic mothers make major challenges worldwide and were not sufficiently studied before. Aim: To investigate the effects of alloxan-induced diabetes on rats' lumbar vertebrae, and to assess the potential beneficial impact of arachidonic acid. Materials and Methods: Pregnant rats were randomly equally divided into four groups: control, alloxan-induced diabetes received alloxan injection 150 mg/kg, alloxan + arachidonic acid group received arachidonic acid 10 μg/animal then given alloxan injection, and arachidonic acid group received it, until offspring age of three weeks. Six male offspring from each group were included in this study at ages of newborn, threeweek-old, two-month-old, and their body measurements were recorded. Lumbar vertebrae and pancreas specimens were examined by light microscopy, morphometry, transmission electron microscopy (TEM), and immunohistochemistry for insulin expression. Results: In alloxaninduced diabetes newborn, three-week-old, and two-month-old rats, body measurements were significantly declined, histomorphometry of 6 th lumbar vertebrae revealed disorganized chondrocytes, with vacuolated cytoplasm, empty lacunae, diminished matrix staining, with areas devoid of cells. TEM showed shrunken reserve and proliferative cells, with irregular nuclei, and damaged mitochondria. In contrast, alloxan + arachidonic acid group had cytoarchitecture of lumbar vertebrae that were like control group. Histomorphometry of pancreas in alloxaninduced diabetes group showed significant reduction in pancreatic islets number and surface area, damaged pancreatic islet cells appeared atrophied with apoptotic nuclei, and very weak insulin immunostaining. Whereas alloxan + arachidonic acid group displayed healthy features of pancreatic islets, which resembled control group, with strong insulin immunostaining. Conclusions: Arachidonic acid mitigated alloxaninduced diabetes by its antidiabetic activity.