Maternal Diabetes Alters Transcriptional Programs in the Developing Embryo

doi:10.1201/b12877-4

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Yuan et al () observed that expression of Hsp105 was mainly in the luminal epithelium on day 1 of pregnancy, and reached a peak level on day 5, whereas in stroma cells, adjacent to the implanting embryo, the strongest expression of Hsp105 was observed on day 6. It was speculated that temporal and spatial changes in Hsp105 expression in pregnant rat uterus may play a physiological role in regulating embryo implantation (Yuan et al, ). In this study, we observed that Hsp70 mainly distributed in decidual‐stromal cells on day 3 of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages

Özaydin

Sur

Öznurlu

et al. 2016

Microscopy Res & Technique

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The aim of the present study was to investigate immunohistochemical distribution of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the mouse placenta at different gestational stages. For this purpose a total of 18 Swiss albino female mice at 12-14 weeks of age were used. Females were sacrificed on days 3 (early), 10 (mid-), and 17 (late) of pregnancy and the implantation sites of the pregnant uterus were sampled. The sections were made transversely through the central region of the implantation site and stained with hematoxylin and eosin for histological examination. PCNA and Hsp70 was stained immunohistochemically. Since the definitive placenta was not still formed on day 3 of pregnancy, Hsp70 and PCNA positivity were evaluated in only luminal epithelium and decidual-stromal cells. On days 10 and 17 of pregnancy, Hsp70 and PCNA positivity were evaluated in labyrinth zone, junctional zone and decidual layer of placenta. Hsp70 expression was observed trophoblast cells and decidual cells and was relatively constant throughout the pregnancy. This protein was strongly labeled in the trophoblast cells; while decidual cells were displayed moderate staining. In early pregnant mouse uteri, PCNA was mainly localized in decidual-stromal cells. The trophoblast cells and decidual cells displayed highly proliferative activity at the midgestational period. However there was a significant decrease in the percentage of PCNA positive cells in late gestation.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages

Özaydin

Sur

Öznurlu

et al. 2016

Microscopy Res & Technique

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show abstract

“…However, the expression IK cytokine protein in pseudopregnant mouse endometrium was markedly lower than that in normal pregnant status and no peak level was observed during the whole pseudopregnant period. It was known that oligodeoxynucleotide can be effectively absorbed by the uterine luminal and glandular epithelium and stroma cells (23). Antisense oligodeoxynucleotide is known to have a half life of 24-48 h in certain tissues (24).…”

Section: Discussionmentioning

confidence: 99%

Characterization of IK cytokine expression in mouse endometrium during early pregnancy and its significance on implantation

Shao

Liu

Ding

et al. 2012

International Journal of Molecular Medicine

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The expression of IK cytokine was investigated in the mouse endometrium during early pregnancy (D1-D7 of pregnancy) and pseudopregnancy using real-time PCR, western blotting and immunohistochemical analysis, and the effects of IK cytokine on embryo implantation were observed by injection with antisense IK cytokine oligodeoxynucleotides in the uterine horn. Our data showed that the expression of IK cytokine mRNA increased gradually from D1 to D4 of pregnancy and reached a peak level at D4 of pregnancy (P<0.05). Western blotting and immunohistochemical analysis revealed that the expression of IK cytokine protein increased gradually from D1 to D5 of pregnancy and reached a peak level at D5 of pregnancy (P<0.05). The expression of IK cytokine in the pseudopregnant uterus was significantly lower compared to that in the normal pregnant uterus and the level of the protein never showed a high peak during the whole pseudopregnancy. The expression of IK cytokine at the implantation site was much stronger than that in the peri-implantation site on Day 5 of pregnancy. After 24 and 48 h of injection with antisense IK cytokine oligodexynucleotides in the uterine horn on D3 of pregnancy (i.e. implantation window), the expression of IK cytokine in the uterus was remarkably inhibited, while the expression of major histocompatibility complex II (MHC II) increased and the number of implanted embryos significantly decreased in the site of uterine horns receiving antisense IK cytokine (P<0.05). These results suggested that IK cytokine may play a crucial role in implantation.

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“…Once embryos attach on the uterus, endometrial stromal cells initiate specialized transformation named decidualization, accompanying the angiogenesis and inflammatory responses [3,4]. Many heat shock proteins (HSP) are evoked during decidualization, including small heat shock protein family [5–8].…”

Section: Introductionmentioning

confidence: 99%

Crystallin αB acts as a molecular guard in mouse decidualization: Regulation and function during early pregnancy

et al. 2014

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Although decidualization is crucial for the establishment of successful pregnancy, the molecular mechanism underlying decidualization remains poorly understood. Crystallin αB (CryAB), a small heat shock protein (sHSP), is up-regulated and phosphorylated in mouse decidua. In mouse primary endometrial stromal cells, CryAB is induced upon progesterone treatment via HIF1α. In addition, CryAB is strongly phosphorylated through the p38-MAPK pathway under stress or during in vitro decidualization. Knockdown of CryAB results in the increase of apoptosis of stromal cells and inhibits decidualization under oxidative or inflammatory stress. Our data indicate that CryAB protects decidualization against stress conditions.

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Maternal Diabetes Alters Transcriptional Programs in the Developing Embryo

Abstract: Background: Heat shock proteins (Hsps) are a set of highly conserved proteins, Hsp105, has been suggested to play a role in reproduction.

Cited by 3 publications

References 13 publications

Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages

Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages

Characterization of IK cytokine expression in mouse endometrium during early pregnancy and its significance on implantation

Crystallin αB acts as a molecular guard in mouse decidualization: Regulation and function during early pregnancy

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