Maternal Dietary Betaine Prevents High-Fat Diet-Induced Metabolic Disorders and Gut Microbiota Alterations in Mouse Dams and Offspring From Young to Adult

Liu, Jieying; Ding, Liang; Zhai, Xiao; Wang, Dongmei; Xiao, Cheng; Hui, Xiangyi; Sun, Tianshu; Yu, Miao; Zhang, Qian; Li, Ming; Xiao, Xinhua

doi:10.3389/fmicb.2022.809642

Cited by 8 publications

(13 citation statements)

References 62 publications

(84 reference statements)

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“…Blood glucose levels were monitored from tail bleeding before intervention and 15, 30, 60, 90, or further 120 min after intervention using a glucometer (FreeStyle Optium™, Abbott, United States). The area under the curve (AUC) was calculated as previously described ( Liu et al, 2022a ).…”

Section: Methodsmentioning

confidence: 99%

“…An ELISA kit (ELR-Insulin-1, RayBiotech Life, Inc., Atlanta, United States) was used to detect plasma insulin levels. Plasma glucose, total triglyceride (TG), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) were measured using an autoanalyzer in Peking Union Medical College Hospital, as previously described ( Liu et al, 2022a ). Insulin sensitivity was analyzed using the homeostasis model assessment of insulin resistance (HOMA-IR).…”

Section: Methodsmentioning

confidence: 99%

“…The DNA extraction and PCR amplification of the cecal content samples and the Illumina NovaSeq platform sequencing are described in our previous studies ( Liu et al, 2022a ). The 16S rDNA genes in distinct regions (16S V3-V4) were amplified using specific primers (341F, CCTAYGGGRBGCASCAG; 806R, GGACTACNNGGGTATCTAAT) and barcodes.…”

Section: Methodsmentioning

confidence: 99%

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Potential benefits of metformin and pioglitazone combination therapy via gut microbiota and metabolites in high-fat diet-fed mice

et al. 2022

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Metformin and pioglitazone monotherapy have been proven to alter gut microbiota in diabetes and obesity. The present study aimed to investigated whether the combined administration of pioglitazone and metformin achieved superior protective effects on high-fat diet (HFD)-fed obese mice and elucidated its molecular mechanism via the gut microbiota and its metabolites. C57BL/6 males were randomly divided into five groups: the control group, fed a normal control diet; the HFD group, fed an HFD; the metformin monotherapy group, fed an HFD and treated with metformin; the pioglitazone monotherapy group, fed an HFD and treated with pioglitazone; and the combination therapy group, fed an HFD and treated with metformin and pioglitazone combination therapy. The cecal contents were collected for 16S rDNA amplicon sequencing and untargeted metabolomics analysis. The results showed that the combination therapy of metformin and pioglitazone significantly improved insulin sensitivity and glucolipid metabolism in HFD-fed mice. Combination therapy markedly altered gut microbiota by increasing beneficial bacteria, such as Bifidobacterium, Christensenellaceae_R-7_group, Faecalibacterium and Roseburia, and decreasing harmful bacteria, such as Oscillibacter and Eubacterium_xylanophilum_group. Fecal metabolites were significantly changed in the combination therapy group, including a reduction in amino acid metabolism and augmentation of lipid metabolism, such as citrulline, sarcosine, D-glutamine, lipoxin A4, prostaglandin E2, stearidonic acid and lucidenic acid A. These results revealed that combined metformin and pioglitazone therapy had synergistic effects or at least have an additive effect on modifying gut microbiota and metabolites, closely associated with improved glucolipid metabolic parameters in HFD-fed mice, which provides novel evidence and promising targets for metformin and pioglitazone combination therapy in type 2 diabetes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Potential benefits of metformin and pioglitazone combination therapy via gut microbiota and metabolites in high-fat diet-fed mice

et al. 2022

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“…On the 5th and 15th days after weaning, FMT treatment increased the relative abundance of Ruminococcus in rectal feces, which was conducive to the maintenance of calf intestinal health. Romboutsia can utilize mixtures of sugars and peptides to produce short chain fatty acids such as isobutanoic acid and acetic acid (Wang et al, 2015;Liu et al, 2022). Short chain fatty acids play an important role in the maintenance of intestinal mucosal barrier, the regulation of intestinal motility, and immune regulation (Feng et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effects of fecal microbiota transplantation from yaks on weaning diarrhea, fecal microbiota composition, microbial network structure and functional pathways in Chinese Holstein calves

et al. 2022

Front. Microbiol.

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This study was conducted to investigate the effect of fecal microbiota transplantation (FMT) from yaks on weaning diarrhea, fecal microbiota composition, microbial network structure and functional pathways in Chinese Holstein Calves. In this study, 50 calves were randomly divided into five groups of 10 each: NC group (no supplementation), Control group (normal saline), low concentration FMT group (LFMT, 1 × 108 CFU/ml), high concentration FMT group (HMFT, 1 × 109 CFU/ml), and sterilized FMT group (SMFT, sterilized bacterial solution). The test lasted for 30 days. We found that FMT reduced the incidence of diarrhea in weaned calves, and the anti-diarrhea effect of LFMT was stronger than those of HFMT and SFMT. Calf feces were collected by rectal palpation on days 5, 10, 15, and 20 post-weaning, and high-throughput sequencing of bacterial 16S rRNA and fungal internal transcribed spacer region of fecal microbiota was performed. We observed that the richness and diversity of bacterial microbiota in the LFMT, HFMT, and SFMT groups were higher than those in the NC and Control groups at day 20 after weaning. The treatment had a significant effect on bacterial richness (p < 0.05), but not on fungal diversity or richness. The analysis of gut microbiome showed that Firmicutes and Bacteroides were the main bacterial phyla in the feces of weaned calves, and norank_ f Muribaculaceae, UCG-005, Rikenellaceae_RC9_gut_group, Bacteroides, and Blautia were the main genera. Ascomycota and Basidiomycota were the main fungal phyla. Compared to abundance parameters in the Control and NC groups, relative abundances of Firmicutes in the FMT groups increased at different time points after weaning. The relative abundance of Blautia and Lactobacillus in the LFMT group increased significantly after weaning. In addition, abundances of Ruminococcus and Romboutsia, which produce short-chain fatty acids, were also increased in different FMT groups. FMT significantly increased the relative abundance of beneficial bacteria, enhanced the complexity of the fecal microbial network, and promoted important metabolic and cellular processes in weaned calves. In conclusion, our study provides a reference and theoretical basis for FMT to prevent calf weaning diarrhea and other intestinal diseases in ruminants.

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“…More betaine intake during lactation led to higher betaine content in the breastmilk, thus an increased the abundance of AKK in offspring’ feces, leading to lower adiposity [ 22 ]. Maternal betaine supplementation alleviated glucose metabolism disorders in 4- and 20-week-old offspring mice induced by MHFD, accompanied by persistent alteration of the gut microbiota [ 23 ]. However, whether the protective effects of maternal betaine intake on NAFLD in offspring are related to gut microbiota regulation and its related metabolites is still unknown.…”

Section: Introductionmentioning

confidence: 99%

Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota

Sun

Tan

et al. 2023

Nutrients

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Maternal betaine supplementation has been proven to alleviate non-alcoholic fatty liver disease (NAFLD) in offspring caused by maternal high-fat diet (MHFD). The gut–liver axis plays an important role in NAFLD pathogenesis. However, whether maternal betaine supplementation can alleviate NAFLD in offspring by the gut–liver axis is unknown. C57BL/6J mice were fed with high-fat diet for 4 weeks before mating, and supplemented with 1% betaine during pregnancy and lactation. After weaning, offspring mice were fed with standard diet to 10 weeks. Maternal betaine supplementation reduced hepatic triglyceride content and alleviated hepatic steatosis in offspring mice exposed to MHFD. Furthermore, the mRNA expression of PPARα, CPT1α and FATP2 was increased and TNFα was reduced by maternal betaine supplementation. Maternal betaine intake decreased the relative abundances of Proteobateria, Desulfovibrio and Ruminococcus, but increased the relative abundances of Bacteroides and Parabacteroides. Moreover, maternal betaine intake increased the concentrations of short-chain fatty acids (SCFAs), including acetic acid, butyric acid and valeric acid, in the feces. Gut microbiota and SCFAs were significantly correlated with hepatic triglyceride content and expression of the above genes. Maternal betaine intake had no effect on other gut microbiota-related metabolites (bile acid and trimethylamine-n-oxide). Altogether, maternal betaine supplementation ameliorated MHFD-induced NAFLD possibly through regulating gut microbiota and SCFAs in offspring mice.

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Maternal Dietary Betaine Prevents High-Fat Diet-Induced Metabolic Disorders and Gut Microbiota Alterations in Mouse Dams and Offspring From Young to Adult

Cited by 8 publications

References 62 publications

Potential benefits of metformin and pioglitazone combination therapy via gut microbiota and metabolites in high-fat diet-fed mice

Potential benefits of metformin and pioglitazone combination therapy via gut microbiota and metabolites in high-fat diet-fed mice

Effects of fecal microbiota transplantation from yaks on weaning diarrhea, fecal microbiota composition, microbial network structure and functional pathways in Chinese Holstein calves

Maternal Betaine Supplementation Mitigates Maternal High Fat Diet-Induced NAFLD in Offspring Mice through Gut Microbiota

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