Epicuticular lipids provide the primary barrier to water loss in insects and other terrestrial arthropods. Using Fourier transform infrared spectroscopy, we found that the melting temperatures for these lipids in the grasshopper Melanoplus sanguinipes varied by over 100C between individuals. The most significant determinant of lipid melting temperature was geographic population, followed by family effects and rearing regime. The width of the phase transition also showed population and family effects. Differences in lipid phase properties were correlated with habitat temperature. Our results provide evidence for genetically based intraspecific variation in epicuticular lipids and have important implications for physiological studies of water balance in arthropods.A rapid increase in water loss above a certain "critical" temperature has led several investigators to conclude that phase transitions of epicuticular lipids are important in determining rates of evaporative water loss in terrestrial arthropods (1-5). According to this hypothesis, as the lipids melt they lose their waterproofing abilities. The biophysical properties of surface lipids necessarily reflect their chemical composition, which is mainly long-chain saturated hydrocarbons, as well as other hydrophobic compounds (6, 7). There are tremendous differences in lipid composition between species, and this variation presumably is due in part to genetic differences, an idea exploited in efforts to use surface lipid composition as a taxonomic character (8). Environmental conditions, particularly temperature, also affect lipid composition, and these changes are correlated with interspecific differences in organismal water balance (9, 10).Surface lipid composition also varies within species, as a result of both genetic (11) and environmental (12, 13) factors. These changes have been used to infer differences in lipid properties, but the actual biophysical consequences of intraspecific variation in lipid composition have not been described. In this study, we used Fourier transform infrared (IR) spectroscopy to study genetic and acclimatory variation in cuticular lipid phase transitions, in cast skins of the lesser migratory grasshopper, Melanoplus sanguinipes.MATERIALS AND METHODS Specimens. The grasshoppers used in these studies were first-generation descendants of individuals collected from the field in the summer of 1989. Grasshoppers from 18 populations all over California were studied (Table 1, Fig. 1). Field-caught animals were maintained and bred in the laboratory, and the eggs were collected and hatched. Nymphs were reared on a diet of lettuce and wheat bran under three environmental regimes: summer = 34WC, 15:9 LD (15 hr of light and 9 hr of dark); fall = 29"C, 11:13 LD; average = 32"C, 13:11 LD. Summer and fall conditions were chosen to simulate actual field conditions during these seasons, as indicated by weather station data (14). Cast skins (exuvae) were collected daily and were stored at -20'C under N2 until analyzed. Storage had no appar...