Maternal-fetal hepatic and placental metabolome profiles are associated with reduced fetal growth in a rat model of maternal obesity

Mumme, Karen; Gray, Charles M.; Reynolds, Clare M.; Vickers, Mark H.; Harrison, Claudia J.; Stanley, John C.; Ruggiero, Katya; Villas‐Bôas, Silas G.; Baker, Philip N.; Sulek, Karolina

doi:10.1007/s11306-016-1014-9

Cited by 4 publications

(2 citation statements)

References 42 publications

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“…To date, there have been several metabolomics studies on both human and murine placental tissue samples [16][17][18][19][20] . In most cases however, multiple analytical platforms, including GC-MS, UPLC-MS and/or NMR were needed to achieve adequate coverage due to the chemical diversity of the placental metabolome, which ranges from polar/hydrophilic amino acids to lipophilic long-chain fatty acids.…”

Section: Discussionmentioning

confidence: 99%

“…Due to its sensitivity to changes in environmental and physiological stimuli, metabolomics provides a link between biochemical mechanisms and molecular phenotype that is closely associated with clinical outcomes 14 . To date, adaptive metabolic changes within placentae during pregnancy have been analyzed in the context of adverse in utero environments, such as pregnancy complications (e.g., preeclampsia, hypoxia, gestational diabetes) and maternal obesity 13,[15][16][17][18][19][20] . Herein, we investigated the impact of sex on the placental metabolome in normal uncomplicated murine pregnancies using multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS).…”

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confidence: 99%

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Placental Metabolomics for Assessment of Sex-specific Differences in Fetal Development During Normal Gestation

Saoi

Kennedy

Gohir

et al. 2020

Sci Rep

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The placenta is a metabolically active interfacial organ that plays crucial roles in fetal nutrient delivery, gas exchange and waste removal reflecting dynamic maternal and fetal interactions during gestation. There is growing evidence that the sex of the placenta influences fetal responses to external stimuli in utero, such as changes in maternal nutrition and exposure to environmental stressors. However, the exact biochemical mechanisms associated with sex-specific metabolic adaptations during pregnancy and its link to placental function and fetal development remain poorly understood. Herein, multisegment injection-capillary electrophoresis-mass spectrometry is used as a high throughput metabolomics platform to characterize lyophilized placental tissue (~2 mg dried weight) from C57BL/6J mice fed a standardized diet. Over 130 authentic metabolites were consistently measured from placental extracts when using a nontargeted metabolomics workflow with stringent quality control and robust batch correction. Our work revealed distinct metabolic phenotype differences that exist between male (n = 14) and female (n = 14) placentae collected at embryonic day E18.5. Intracellular metabolites associated with fatty acid oxidation and purine degradation were found to be elevated in females as compared to male placentae (p < 0.05, effect size >0.40), including uric acid, valerylcarnitine, hexanoylcarnitine, and 3-hydroxyhexanolycarnitine. This murine model sheds new insights into sexspecific differences in placental mitochondrial function and protective mechanisms against deleterious oxidative stress that may impact fetal growth and birth outcomes later in life.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Placental Metabolomics for Assessment of Sex-specific Differences in Fetal Development During Normal Gestation

Saoi

Kennedy

Gohir

et al. 2020

Sci Rep

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Preliminary metabolomics analysis of placenta in maternal obesity

et al. 2018

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Placental metabolome analysis of obese pregnancies showed differences in metabolites involved in antioxidant defenses, nucleotide production, as well as lipid synthesis and energy production, supporting a shift towards higher placental metabolism. OB placentas also showed a specific fatty acids profile suggesting a disruption of LC-PUFA biomagnification. This study can lay the foundation to further metabolomic placental characterization in maternal obesity. Metabolic signatures in obese placentas may reflect changes occurring in the intrauterine metabolic environment, which may affect the development of adult diseases.

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