Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets

Tomaszewska, Ewa; Dobrowolski, Piotr

doi:10.2478/v10213-012-0044-y

Cited by 10 publications

(10 citation statements)

References 38 publications

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“…Earlier studies have shown that, in studies in experimentally DEX-induced IUGR, term body weight can be various. Newborns can weigh more or less (1716-1197 g) compared with not retarded controls depending on the DEX dose, period of the pregnancy, and duration of the administration [9,12,[15][16][17][18]20,51]. Thus, only reduced rate of weight gain, changes in the skeletal system, or changes in the intestinal tract observed later in life in experimental pigs indicated the existence of IUGR.…”

Section: Discussionmentioning

confidence: 99%

“…High concentrations of endogenous GCs are observed during the fetal period, following either short-term or chronic stress of a pregnant female (transport stress, social stress, and abnormal living conditions). Studies on prenatal programming have shown that GCs primarily inhibit fetal growth and reduce placental size, depending on the concentration of GCs and the duration of exposure [14,15]. There are several animal models of experimentally-induced IUGR, one of which involves the use of GCs.…”

Section: Introductionmentioning

confidence: 99%

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Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?

Tomaszewska

Dobrowolski

Świątkiewicz

et al. 2020

Animals

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In this study, the effect of long-term 2-oxoglutaric acid (2-Ox) supplementation to experimentally-induced intrauterine growth retarded gilts was examined. Sows were treated with synthetic glucocorticoid (dexamethasone) every second day, during the last 45 days of pregnancy, at a dose of 0.03 mg/kg b.w. At birth, the gilts were randomly divided into two groups: unsupplemented and supplemented with 2-Ox for nine months (0.4 g/kg body weight/day). Oral supplementation of 2-Ox to experimentally-induced intrauterine growth retarded gilts increased body weight at weaning as well as final body weight at the age of nine months, and showed a regenerative effect on bone mineralization and morphology of trabeculae and articular cartilage. The positive effects on bone structure were attributed to the 2-Ox-induced alterations in bone metabolism, as evidenced by the changes in the expression of proteins involved in bone formation and remodeling: osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor kappa-Β ligand (RANKL), tissue inhibitor of metalloproteinases 2 (TIMP-2), bone morphogenetic protein 2 (BMP-2), cartilage oligomeric matrix protein (COMP), and vascular endothelial growth factor (VEGF).

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?

Tomaszewska

Dobrowolski

Świątkiewicz

et al. 2020

Animals

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“…Previous studies have indicated that nutritional or hormonal factors acting during prenatal time can programme skeletal or dental development (Śliwa, Dobrowolski, & Piersiak, ; Śliwa, Tatara, Nowakowski, Pierzynowski, & Studziński, ; Śliwa et al., ; Tomaszewska & Dobrowolski, ; Tomaszewska, Dobrowolski, & Siwicki, ). HMB is one of the factors that play a significant role in prenatal modulation of subsequent postnatal growth (Blicharski et al., ; Świetlicka et al., ; Tatara, Śliwa, & Krupski, ).…”

Section: Discussionmentioning

confidence: 99%

Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin system

Tomaszewska

Muszyński

Dobrowolski

et al. 2019

Animal Physiology Nutrition

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It has been demonstrated in animal studies that prenatal administration of βhydroxy-β-methylbutyrate (HMB, metabolite of leucine) influences general growth and mechanical endurance of long bones in newborn offspring in sex-dependent manner. The present experiment was conducted to evaluate the effect of HMB treatment of pregnant sows on bone development in offspring at weaning. From 70th day until the 90th day of gestation, sows received either a basal diet (n = 12) or the same diet supplemented with HMB (n = 12) at the dose of 0.2 g/kg of body weight/day. Femora obtained from six males and females in each group weaned at the age of 35 days were examined. Maternal HMB treatment significantly enhanced body weight and changed bone morphology increasing femur mechanical strength in both sexes. Maternal HMB supplementation also elevated bone micro-and macroelement concentrations and enhanced content of proteoglycans in articular cartilage. Based on the obtained results, it can be concluded that maternal HMB supplementation in the mid-gestation period significantly accelerated bone development in both sexes by upregulation of a multifactorial system including leptin and osteoprotegerin.However, the sex (irrespective of the HMB treatment) was the factor which influenced the collagen structure in cartilages and trabecular bone, as demonstrated both by the Picrosirius red staining and performed analysis of thermal stability of collagenous tissues. The structural differences in collagen between males and females were presumably related to a different collagen maturity. No studies conducted so far provided a detailed morphological analysis of bone, articular cartilage, growth plate and the activities of the somatotropic and pituitary-gonadal axes, as well as leptin/osteoprotegerin system in weaned offspring prenatally treated with HMB. This study showed also the relationship between the maternal HMB treatment and bone osteometric and mechanical traits, hormones, and growth and bone turnover markers such as leptin, osteoprotegerin and insulin-like growth factor-1. K E Y W O R D Sbone, cartilage, offspring, pigs, pregnancy, weaning, β-hydroxy-β-methylbutyrate | 627 TOMASZEWSKA ET Al.

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“…This HMB dose was selected based on previous studies that used the same dose of hydroxy-β-methylbutyrate and had a significant effect on offspring development [16,17,27]. In this study we decided to supplement sows between 70 to 90 days of pregnancy, because this is critical moment to prenatal development in piglets [28].…”

Section: Experimental Modelmentioning

confidence: 99%

The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets

Tomczyk-Warunek

Blicharski

Jarecki

et al. 2020

Preprint

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The presented experiment focuses on assessing the impact of HMB ( hydroxy-β-methobutyrate ) supplementation of mothers during pregnancy on the development of the skeletal system of their offspring. For this purpose, an experiment was carried out on 12 clinically healthy sows of the Great White Poland breed, which were divided randomly into two groups the control and the HMB group. All animals were kept under standard conditions and received the same feed for pregnant females. In contrast, females from the HMB group between 70 and 90 days were supplemented with 3-hydroxy-3-methylbutyle in the amount of 0.2g/kg b.w/day. Immediately after birth, the piglets were also divided into groups based on: sex, and presence or lack HMB supplementation, and subsequently were euthanized and humerus bones from all piglets were collected. Mother’s HMB supplementation during pregnancy affected the multiple index of their offspring. The higher humerus mass and length was observed with the greater effect in males. Maternal supplementation also influenced on the geometrical and mechanical properties of the humerus as in the case of mass, this effect was higher in males. Also, the collagen structure of the compacted and trabecular bone changed under the HMB addition. Maternal supplementation also affected the expression of selected proteins in growth cartilage and trabecular bone. The obtained results show that the administration to the mother during pregnancy by the HMB significantly affects the development of the humerus in many ways. The obtained results also confirm the utility of such experiments in understanding of the importance of the pregnancy diet as an develop and adaptable factor of offspring organisms and are the base for further research in that area as well as in the protein markers expression area.

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Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets

Cited by 10 publications

References 38 publications

Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?

Is Dietary 2-Oxoglutaric Acid Effective in Accelerating Bone Growth and Development in Experimentally-Induced Intrauterine Growth Retarded Gilts?

Maternal HMB treatment affects bone and hyaline cartilage development in their weaned piglets via the leptin/osteoprotegerin system

The effect of maternal HMB supplementation on bone mechanical and geometrical properties, as well as histomorphometry and immunolocalization of VEGF, TIMP2, MMP13, BMP2 in the bone and cartilage tissue of the humerus of their newborn piglets

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