Maternal high-fat diet alters expression of pathways of growth, blood supply and arachidonic acid in rat placenta

Nitert, Marloes Dekker; Vaswani, Kanchan; Hum, Melissa; Chan, Hsiu‐Wen; Wood-Bradley, Ryan J; Henry, Sarah; Armitage, James A.; Mitchell, Murray D.; Rice, Gregory E.

doi:10.1017/jns.2013.36

Cited by 7 publications

(5 citation statements)

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“…Four time points were chosen as they cover the time period from mid gestation to the day prior to labour onset (E14.25, E15.25, E17.25 and E20). These investigations are apart of a larger gene expression study [ 28 , 29 ]. The rat model allows the study of RAS compenents at time-points (mid to late gestation) difficult to be obtained from human and unlike in the mouse, placentation in the rat involves the deep invasion of trophoblast ( i.e.…”

Section: Introductionmentioning

confidence: 99%

The rat placental renin-angiotensin system - a gestational gene expression study

Vaswani

Chan

Verma

et al. 2015

Reprod Biol Endocrinol

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BackgroundThe placenta is an essential organ that provides nutrients and oxygen to the developing fetus and removes toxic waste products from the fetal circulation. Maintaining placental blood osmotic pressure and blood flow is crucial for viable offspring. The renin-angiotensin system (RAS) in the placenta is a key player in the regulation of maternal-fetal blood flow during pregnancy. Therefore, the aim of this study was to determine if RAS genes are differentially expressed in mid to late gestation in rat placenta.MethodsWhole placental tissue samples from pregnant Sprague Dawley rats at embryonic (E) days 14.25, 15.25, 17.25 and 20 (n = 6 for each gestational age) were used for genome-wide gene expression by microarray. RAS genes with expression differences of >2 fold were further analyzed. Quantitative Real-Time PCR (qPCR) was performed on independent samples to confirm and validate microarray data. Immunohistochemisty and Western blotting were performed on a differentially expressed novel RAS pathway gene (ANPEP).ResultsSix out of 17 genes of the RAS pathway were differentially expressed at different gestational ages. Gene expression of four genes (Angiotensin converting enzyme (Ace), angiotensin converting enzyme 2 (Ace2), membrane metalloendopeptidase (Mme) and angiotensin II receptor 1A (Agtr1a)) were significantly upregulated at E20 whereas two others (Thimet oligopeptidase 1 (Thop1) and Alanyl aminopeptidase (Anpep)) were downregulated at E20 prior to the onset of labour. These changes were confirmed by qPCR. Western blots revealed no overall differences in ANPEP protein expression in the placentae. Immunohistochemical studies, however, indicated that the localization of ANPEP differed at E17.25 and E20 as ANPEP localization in the giant trophoblast cell of the junctional zone was no longer detectable at E20.ConclusionsThe current study investigated the expression of members of the RAS pathway in rat placentae and observed significantly altered expression of 6 RAS genes at 4 gestational ages. These findings present the need for further comprehensive investigation of RAS genes in normal and complicated pregnancies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0088-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

The rat placental renin-angiotensin system - a gestational gene expression study

Vaswani

Chan

Verma

et al. 2015

Reprod Biol Endocrinol

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“…Maternal high-fat diet upregulates the expression of placental mRNA in the arachidonic acid metabolism pathway. ( 20 ) The increase in maternal arachidonic acid that accompanies a high-fat diet would be expected to increase the fetal arachidonic acid level, which would thus upregulate the fetal expression of VEGF. Overexpression of VEGF in the myocardium inhibits the process of epithelial-mesenchymal transformation responsible for endocardial cushion formation.…”

Section: Discussionmentioning

confidence: 99%

Maternal dietary fat intake and the risk of congenital heart defects in offspring

et al. 2020

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Background Fatty acids are crucial in embryologic development, including cardiogenesis. The impact of maternal periconceptional dietary fat intake on the risk of congenital heart defects (CHDs) has not been clearly elucidated. We hypothesized that maternal dietary fat intake during pregnancy is associated with risk of CHDs in offspring. Methods We analyzed CHD cases and non-malformed controls from the National Birth Defects Prevention Study, a case-control, multicenter population-based study of birth defects. We used multivariable logistic regression to analyze the association between maternal periconceptional dietary fat intake and occurrence of CHDs. Results We examined 11,393 infants with CHDs (cases) and 11,029 infants without birth defects (controls). Multivariable analysis of maternal dietary fat intake adjusted for maternal energy intake demonstrated modest change in risk for 2 of the 25 CHDs analyzed; otherwise there was no association. Maternal dietary fat intake unadjusted for total energy was associated with increased risk for several CHDs. Conclusion After adjusting for total energy intake, maternal periconceptional dietary fat intake has a modest association with risk of a few specific CHDs. If maternal dietary fat intake does impact CHD risk, the effect is minimal.

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“…However, studies have demonstrated that a diet high in saturated fats, often found in processed foods containing MG, may indeed increase the risk of developing CHD. High-fat diets in expectant mothers have been shown to enhance the expression of placental mRNA associated with the arachidonic acid (AA) metabolism pathway 43 . This can lead to elevated fetal arachidonic acid levels due to maternal arachidonic acid transfer, resulting in an upregulation of fetal VEGF expression 44 .…”

Section: Monoradylglycerols (Mg) Are a Type Of Lipid Molecule Charact...mentioning

confidence: 99%

A Metabolic Biomarker Panel for Congenital Heart Disease Assessment with Newborn Dried Blood Spots

Ceresnac,

Zhang,

et al. 2023

Preprint

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Background: Congenital heart disease (CHD) represents a significant contributor to both morbidity and mortality in neonates and children. The prompt recognition of CHD can facilitate timely and appropriate intervention, reducing the probability of complications and enhancing the prognosis for impacted newborns. However, unlike other rare conditions routinely identified through federal and state newborn screening (NBS) programs, there's currently no analogous dried blood spot (DBS) screening for CHD immediately after birth. Objective: This study was set to identify reliable metabolite biomarkers with clinical relevance, with the aim to assess feasibility of screening and subtype classification of CHD utilizing the DBS newborn screening method. Methods: We assembled a cohort of DBS datasets from the California Department of Public Health (CDPH) Biobank, encompassing both normal controls and three pre-defined CHD categories (tetralogy of Fallot, inherited arrhythmia syndrome, neonatal cardiomyopathy). A robust, DBS-oriented metabolomic method, employing both global and targeted strategies based on liquid chromatography with tandem mass spectrometry (LC-MS/MS), was developed. To verify the reliability of this metabolic profiling, we conducted a correlation analysis comparing the absolute quantitated metabolite concentration in DBS against the CDPH NBS records. Additionally, for hydrophilic and hydrophobic metabolites, we executed significant pathway and metabolite analyses respectively. Finally, logistic and LightGBM models were established to aid in CHD discrimination and classification. Results: Our metabolomic workflow demonstrated consistent and reliable quantification of metabolites in DBS samples stored at the California Department of Public Health (CDPH) for up to 15 years. Through this process, we discerned dysregulated metabolic pathways in CHD patients, including deviations in lipid and energy metabolism, as well as oxidative stress pathways. Furthermore, we identified three metabolites as potential biomarkers for CHD assessment, and an additional twelve metabolites as potential markers for classifying different CHD subtypes within DBS samples. Conclusions: This study represents the first attempt to validate metabolite profiling results using long-term storage DBS samples procured from the high-quality conditions of the CDPH biobank. The results unveil distinct metabolic discrepancies between various CHD subtypes and healthy controls. Furthermore, our findings highlight the potential clinical applications of our DBS-based methods for CHD screening and subtype classification.

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Maternal high-fat diet alters expression of pathways of growth, blood supply and arachidonic acid in rat placenta

Cited by 7 publications

References 29 publications

The rat placental renin-angiotensin system - a gestational gene expression study

The rat placental renin-angiotensin system - a gestational gene expression study

Maternal dietary fat intake and the risk of congenital heart defects in offspring

A Metabolic Biomarker Panel for Congenital Heart Disease Assessment with Newborn Dried Blood Spots

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