Maternal hypomagnesemia causes placental abnormalities and fetal and postnatal mortality

Schlegel, RobertJ.; Cuffe, James; Moritz, Karen M.; Paravicini, Tamara M.

doi:10.1016/j.placenta.2015.03.011

Cited by 40 publications

(42 citation statements)

References 39 publications

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“…In Mg-deficient offspring, body weight was reduced at PN8 and PN15 as we have previously reported [5]. This coincides with the crucial period of NMDAR maturation, during which significant changes in GluN2 receptor expression normally occur [11] .…”

Section: Discussionsupporting

confidence: 81%

“…These findings are supported by the US National Health and Nutritional Examination Survey data from the period 2001 -2006, which reported that 64% of women aged 19 -30 consumed less than the estimated average requirement for Mg [2]. Recently, we found that maternal Mg deficiency adversely affected offspring by impairing placental growth and fetal development in an animal model of dietary Mg deficiency [5]. Although experimental studies in rodents have shown that maternal micronutrient deficiencies can program for altered neurophysiology and behavioural deficits in offspring [6,7], no studies have investigated long-term neurobehavioural outcomes following maternal Mg deficiency.…”

Section: Introductionsupporting

confidence: 67%

“…Thus, the behavioural changes are due to altered Mg availability during a critical period of development, and are not due to reduced Mg levels in the adult offspring. Interestingly, we have previously shown that while moderate Mg deficiency reduces plasma Mg in the dam by ~ 40%, the fetal plasma Mg levels are maintained in a normal range during late gestation [5]. Despite this, the reduced maternal Mg availability during gestation had a significant and long-lasting impact upon brain development and behaviour in the adult offspring.…”

Section: Discussionmentioning

confidence: 98%

“…This low Mg diet has been shown to significantly reduce plasma Mg concentrations in pregnant mice [5]. Offspring were weaned at PN21 and placed on a standard laboratory rodent chow diet.…”

Section: Animal Treatmentmentioning

confidence: 99%

“…In this study, we used a model of moderate maternal Mg deficiency [5] to evaluate the effects of gestational Mg deficiency on hippocampal NMDAR expression and behaviour in offspring. We hypothesised that maternal Mg deficiency would alter neonatal NMDAR receptor expression and lead to behavioural changes in the adult offspring.…”

Section: Introductionmentioning

confidence: 99%

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Maternal hypomagnesemia alters hippocampal NMDAR subunit expression and programs anxiety-like behaviour in adult offspring

Schlegel

Spiers

Cullen

et al. 2017

Behavioural Brain Research

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  Maternal Mg deficiency reduced hippocampal GluN1 and GluN2A in male offspring.  Female offspring of Mg-deficient dams had increased GluN1, GluN2A and GluN2B.  Maternal Mg deficiency increased hippocampal GluN2B:GluN2A in adult offspring. AbstractIt is well established that maternal undernutrition and micronutrient deficiencies can lead to altered development and behaviour in offspring. However, few studies have explored the implications of maternal Mg deficiency and programmed behavioural and neurological outcomes in offspring. We used a model of Mg deficiency (prior to and during pregnancy and lactation) in CD1 mice to investigate if maternal Mg deficiency programmed changes in behaviour and NMDAR subunit expression in offspring. Hippocampal tissue was collected at postnatal day 2 (PN2), PN8, PN21 and 6 months, and protein expression of NMDAR subunits GluN1, GluN2A and GluN2B was determined. At 6 months of age, offspring were subject to behavioural tasks testing aspects of anxiety-like behaviour, memory, and neophobia. Maternal hypomagnesemia was associated with increased GluN1, GluN2A and GluN2B subunit expression in female offspring at 6 months, but decreased GluN1 and GluN2A expression in males. The GluN2B:GluN2A expression ratio was increased in both sexes. Male (but not female) offspring from Mg-deficient dams showed anxiety-like behaviour, with reduced head dips (Suok test), and reduced exploration of open arms (elevated plus maze). Both male and female offspring from Mg-deficient dams also showed impaired recognition memory (novel object test). These findings suggest that maternal Mg deficiency can result in behavioural deficits in adult life, and that these changes may be related to alterations in hippocampal NMDA receptor expression.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 98%

“…This low Mg diet has been shown to significantly reduce plasma Mg concentrations in pregnant mice [5]. Offspring were weaned at PN21 and placed on a standard laboratory rodent chow diet.…”

Section: Animal Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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