Maternal identification of hybrid eggs in Hexagrammos spp. by means of multiplex amplified product length polymorphism of mitochondrial DNA

Kimura, Masafumi; Yanagimoto, Takashi; Munehara, Hiroyuki

doi:10.3354/ab00019

Cited by 14 publications

(15 citation statements)

References 13 publications

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“…, ; Kimura and Munehara ), egg masses showing the Hoc haplotype have occasionally been found in Hag territories (Kimura et al. ). The present study revealed that all of the egg masses showing the Hoc haplotype were spawned by hemiclonal hybrids ( Hoc */ Hag ) and not Hoc individuals.…”

Section: Discussionmentioning

confidence: 99%

“…The mtDNA haplotype analysis was performed using the method of Kimura et al. (), with multiplex amplified product length polymorphism (APLP) analysis of the 12S and 16S rRNA (12‐16S rRNA) gene regions. Ampli Taq Gold DNA polymerase (Applied Biosystems, CA) was used to assure high annealing stringency of species‐specific primers to complementary diagnostic sites.…”

Section: Methodsmentioning

confidence: 99%

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Unisexual hybrids break through an evolutionary dead end by two‐way backcrossing

et al. 2020

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Unisexual vertebrates (i.e., those produced through clonal or hemiclonal reproduction) are typically incapable of purging deleterious mutations, and, as a result, are considered short‐lived in evolutionary terms. In hemiclonal reproduction (hybridogenesis), one parental genome is eliminated during oogenesis, producing haploid eggs containing the genome of a single parent. Hemiclonal hybrids are usually produced by backcrossing hemiclonal hybrids with males of the paternal species. When hemiclonal hybrids from a genus of greenlings (Hexagrammos) are crossed with males of the maternal species, the progeny are phenotypically similar to the maternal species and produce recombinant gametes by regular meiosis. The present study was conducted to determine if the hemiclonal genome is returned to the gene pool of the maternal species in the wild. Using a specific cytogenetic marker to discriminate between such progeny and the maternal species, we observed that Hexagrammos hybrids mated with maternal and paternal ancestors at the same frequency. This two‐way backcrossing in which clonal genomes are returned to the gene pool where they can undergo recombination plays an important role in increasing the genetic variability of the hemiclonal genome and reducing the extinction risk. In this way, hybrid lineages may have survived longer than predicted through occasional recombinant generation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Unisexual hybrids break through an evolutionary dead end by two‐way backcrossing

et al. 2020

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“…Fishes were collected by local fishermen and preserved in 95% ethanol until DNA was extracted. For the Japan samples, cytochrome b sequences of 18 samples from two locations, Usujiri (UJ) and Mie (ME), in the Pacific coast of Japan were used (Kimura et al 2007; GenBank accession numbers: AB290743-AB290754).…”

Section: Collection Of Samples and Sequence Datamentioning

confidence: 99%

“…The male fertilizes and takes care of the eggs until hatching (Munehara et al 2000). Several studies have been carried out regarding the morphology, systematics and ecology of the species (Kanamoto 1979;Huh and Kwak, 1997;Crow et al 2004;Kwak et al 2005;Kimura et al 2007). However, studies on the genetic diversity and population structure of H. agrammus have not been conducted yet.…”

Section: Introductionmentioning

confidence: 99%

Population panmixia and the Pleistocene demographic expansion of spotty belly greenling Hexagrammos agrammus in the East Sea and Northwest Pacific

et al. 2015

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 The population genetic structure and historical demography of spotty belly greenling, Hexagrammos agrammus, which has limited distribution in the Northwest Pacific, was assessed with partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and the control region (D-loop). A total of 103 individuals were collected from four sites located at the Korea Strait (Southern coast of Korea) and the East coast of Korea and two places in the Pacific coast of Japan. For all the populations, nucleotide diversities were low (0.006 -0.009) while the haplotype diversities were as high as 0.92 to 0.97, indicating that the fish has undergone a recent population expansion after experiencing bottleneck. Star-shaped patterns of haplotype networks as well as the significant negative values of Tajima's D and Fu's F S corroborate the recent population expansion. Mismatch distribution analysis reveals that the demographic expansion of the species started during the 2 nd half of the Middle Pleistocene Series approximately 141,000 -406,000 years ago. Hierarchical analysis of molecular variance (AMOVA), the pairwise population statistics (F ST ), and the exact test of haplotype differentiation demonstrate no significant genetic differentiation among populations investigated, suggesting that spotty belly greenling is panmictic in the East Sea and the Pacific coast of Japan.

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“…Blood sample was taken from each individual and preserved at -80°C until DNA was extracted. For the Japan fat greening, cytochrome b sequence data of 16 samples were obtained from the dataset of Kimura et al (2007) (GenBank accession numbers: AB290730-AB290742).…”

Section: Sample and Data Collectionmentioning

confidence: 99%

Population genetic structure and demographic history of the fat greenling Hexagrammos otakii

et al. 2011

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Fat greenling (Hexagrammos otakii) is an important commercial fish in the Northwestern Pacific, being distributed along the coastal waters of the East/Japan Sea and the Yellow Sea. To investigate population genetic structure and demographic history of this species, one hundred and fifty five individuals were collected from five localities in the distribution range of the species and sequence variations in the mitochondrial genes COI, COIII-ND3-ND4L, and cytochrome b were examined. For all the genes in every sampling location, the nucleotide diversities were very low (0.001 ~ 0.005) although the haplotype diversities were relatively high, 0.55 ~ 0.81 for COI, 0.79 ~ 0.84 for COIII-ND3-ND4L, and 0.95 ~ 0.97 for cytochrome b. Hierarchical analysis of molecular variance (AMOVA), the conventional population statistic FST, and exact test of population differentiation revealed no significant genetic structuring among the samples, indicating that fat greening is panmictic throughout the range of its distribution. Neutrality tests such as Tajima's D and Fu's FS statistics and mismatch distribution analyses suggested that fat greening has undergone the demographic history of population expansion during the late Pleistocene period approximately 91,000 ~ 327,000 years ago. The star-burst patterns of haplotype networks and low nucleotide diversities also indicated recent population expansion. These results help establish the fisheries management strategy for fat greenling in the Northwestern Pacific.

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Maternal identification of hybrid eggs in Hexagrammos spp. by means of multiplex amplified product length polymorphism of mitochondrial DNA

Cited by 14 publications

References 13 publications

Unisexual hybrids break through an evolutionary dead end by two‐way backcrossing

Unisexual hybrids break through an evolutionary dead end by two‐way backcrossing

Population panmixia and the Pleistocene demographic expansion of spotty belly greenling Hexagrammos agrammus in the East Sea and Northwest Pacific

Population genetic structure and demographic history of the fat greenling Hexagrammos otakii

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