Maternal immunity of tilapia broodstock vaccinated with polyvalent vaccine and resistance of their offspring againstStreptococcus agalactiae

Abstract

This study aimed to examine the use of Streptococcus agalactiae polyvalent vaccine in tilapia broodstock and the effect of maternal immunity and resistance on their offspring against S. agalactiae strain. The broodstock was injected with polyvalent vaccine of S. agalactiae at a dose of 108 CFU per fish at 2nd gonadal maturity until spawning. Challenge test was carried out on the offspring at the 5, 10, 15 and 20 days after hatching using NK1, N17O, N14G, N3M, N4M strain respectively and combination of them. We observed immunological parameters in broodstock, eggs and larvae and relative per cent survival (RPS) of larvae after challenged with pathogenic S. agalactiae. The results showed that the leukocytes, phagocytic activity, respiratory burst, lysozyme activity and antibody levels of vaccinated broodstock had higher level compared with unvaccinated broodstock. The high level of the lysozyme activity, antibody levels and recombination activating gene 1 (RAG1) were also observed in eggs and larvae from vaccinated broodstock. Larvae produced from vaccinated broodstock when challenged with variety strain of pathogenic S. agalactiae had RPS value more than 50% until 20 days after hatching. In conclusion, polyvalent vaccine of S. agalactiae administrated in the broodstock could enhance immunity in the broodstock and protect their offspring from pathogenic S. agalactiae.

Meningitis Caused by Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus): Infection and Inflammatory Response

“…In previous studies, our laboratory has substantiated the use of Nile tilapia as a study model for meningitis caused by Sta [ 14 ] Aeromonas hydrophila [ 28 ] and for acute inflammatory study [ 29 ]. Nurani et al, [ 30 ] proved the effectiveness of using a polyvalent vaccine for Sta and the ability to passively transfer to offspring. In addition to the importance of this study for aquaculture health, these findings may collaborate as a model for the prophylaxis of human neonatal meningitis.…”

Transfer of maternal immunity using a polyvalent vaccine and offspring protection in Nile tilapia, Oreochromis niloticus

“…Following vaccinations, the fish's immune response was observed on the 7 th , 14 th , 21 st , and 28 th day by collecting intramuscular blood samples. The immune response parameters were the antibody titer using the direct agglutination method 26 , total leukocyte 20,22,27 , phagocytic [28][29][30] and lysozyme activities 13,20,29,30 .…”

“…The transfer of maternal immunity using PAPS for S. agalactiae, Lactococcus garvieae, and Enterococcus faecalis has been studied by Abu-elala et al, 19 and three vaccine strains for S. agalactiae by Nurani et al 20 . The types of bacterial diseases studied in the aforementioned studies are very limited even though Nile tilapia often suffer from them in fish farms and hatcheries 21 .…”

“…The types of bacterial diseases studied in the aforementioned studies are very limited even though Nile tilapia often suffer from them in fish farms and hatcheries 21 . Besides being infected by S. agalactiae 15,[20][21][22][23] , Nile tilapia are often infected by A. hydrophila 21,22,24 and P. fluorescens 24,25 leading to high mortality, including in Indonesia. Therefore, this study aims to examine maternal immunity transfer using the vaccines for S. agalactiae, A. hydrophila, and P. fluorescens.…”

Omadacycline efficacy against Streptococcus agalactiae isolated in China: in vitro activity, resistance analysis, clonality of MIC distribution, and the correlation between resistance and virulence gene and biofilm formation

“…S. agalactiae showed resistance to antibiotics mediated by corresponding resistance genes. The e ux pump mechanism, the ribosomal 23SrRNA methylation mechanism [6] , and the ribosomal protective protein effect mechanism [7] regulated by the coding of the tet gene are the three main resistance mechanisms of S. agalactiae. The e ux pump mechanism is the main form of S. agalactiae anti-macrolide drugs, which is expressed in the form of a proton e ux pump mediated by mefA gene, which will expel the macrolide antibiotics out of the cell body, so that the target site drugs.…”