Maternal low protein diet induces persistent expression changes in metabolic genes in male rats

Lira, Allan de Oliveira; Alves, José Luiz; Fernandes, Mariana P.; Vasconcelos, Diogo Antônio Alves de; Santana, David Filipe de; Costa‐Silva, João Henrique; Morio, Béatrice; Leandro, Carol Góis; Pirola, Luciano

doi:10.4239/wjd.v11.i5.182

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…The combined effect of the paternal and maternal HC/LP diet led to dysregulation of energy metabolism at the transcriptomic level, as seen by the overall downregulation of the expression of genes coding for proteins involved in energy production (glycolysis and TCA) in HH fry in comparison to NN fry. Previous studies reported a similar outcome in the muscle of offspring born from mothers fed LP diets in murine species [8,55,56].…”

Section: Energy Metabolism Was Altered By the Parental Hc/lp Dietsupporting

confidence: 64%

Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

Callet

Coste

et al. 2021

Biology

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It is now recognized that parental diets could highly affect offspring metabolism and growth. Studies in fish are, however, lacking. In particular, the effect of a parental diet high in carbohydrate (HC) and low in protein (LP) on progeny has never been examined in higher trophic level teleost fish. Thus, two-year old male and female rainbow trout (Oncorhynchus mykiss) were fed either a control diet (0% carbohydrate and 63.89% protein) or a diet containing 35% carbohydrate and 42.96% protein (HC/LP) for a complete reproductive cycle for females and over a 5-month period for males. Cross-fertilizations were then carried out. To evaluate the effect of the parental diet on their offspring, different phenotypic and metabolic traits were recorded for offspring before their first feeding and again three weeks later. When considering the paternal and maternal HC/LP nutrition independently, fry phenotypes and transcriptomes were only slightly affected. The combination of the maternal and paternal HC/LP diets altered the energy metabolism and mitochondrial dynamics of their progeny, demonstrating the existence of a synergistic effect. The global DNA methylation of whole fry was also highly affected by the HC/LP parental diet, indicating that it could be one of the fundamental mechanisms responsible for the effects of nutritional programming.

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Section: Energy Metabolism Was Altered By the Parental Hc/lp Dietsupporting

confidence: 64%

Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

Callet

Coste

et al. 2021

Biology

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show abstract

“…Besides, the chemically induced carcinogenesis model is an important tool to study the multistep process of mammary carcinogenesis ( Russo and Russo, 1996 ; Russo, 2015 ). Using a maternal LPD model, our research group and others reported important changes in several organs, such as liver, mammary gland, pancreas, prostate, and adipose tissue ( Plank et al, 2006 ; Fernandez-Twinn et al, 2007 ; Beinder et al, 2014 ; Santos et al, 2019 ; Varuzza et al, 2019 ; Alejandro et al, 2020 ; de Oliveira Lira et al, 2020 ). Fernandez-Twinn et al (2007) were the first to demonstrate the adverse effects of gestational and lactational LPD on N -methyl- N -nitrosourea (MNU)–induced mammary carcinogenesis model in female offspring from Wistar rats, using a total of three 50 mg/kg body weight (b.w.)…”

Section: Introductionmentioning

confidence: 91%

Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood

Zapaterini

Fonseca

Bidinotto

et al. 2022

Front. Cell Dev. Biol.

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Studies have shown that maternal malnutrition, especially a low-protein diet (LPD), plays a key role in the developmental mechanisms underlying mammary cancer programming in female offspring. However, the molecular pathways associated with this higher susceptibility are still poorly understood. Thus, this study investigated the adverse effects of gestational and lactational low protein intake on gene expression of key pathways involved in mammary tumor initiation after a single dose of N-methyl-N-nitrosourea (MNU) in female offspring rats. Pregnant Sprague–Dawley rats were fed a normal-protein diet (NPD) (17% protein) or LPD (6% protein) from gestational day 1 to postnatal day (PND) 21. After weaning (PND 21), female offspring (n = 5, each diet) were euthanized for histological analysis or received NPD (n = 56 each diet). At PND 28 or 35, female offspring received a single dose of MNU (25 mg/kg body weight) (n = 28 each diet/timepoint). After 24 h, some females (n = 10 each diet/timepoint) were euthanized for histological, immunohistochemical, and molecular analyses at PDN 29 or 36. The remaining animals (n = 18 each diet/timepoint) were euthanized when tumors reached ≥2 cm or at PND 250. Besides the mammary gland development delay observed in LPD 21 and 28 groups, the gene expression profile demonstrated that maternal LPD deregulated 21 genes related to DNA repair and DNA replication pathways in the mammary gland of LPD 35 group after MNU. We further confirmed an increased γ-H2AX (DNA damage biomarker) and in ER-α immunoreactivity in mammary epithelial cells in the LPD group at PND 36. Furthermore, these early postnatal events were followed by significantly higher mammary carcinogenesis susceptibility in offspring at adulthood. Thus, the results indicate that maternal LPD influenced the programming of chemically induced mammary carcinogenesis in female offspring through increase in DNA damage and deregulation of DNA repair and DNA replication pathways. Also, Cidea upregulation gene in the LPD 35 group may suggest that maternal LPD could deregulate genes possibly leading to increased risk of mammary cancer development and/or poor prognosis. These findings increase the body of evidence of early-transcriptional mammary gland changes influenced by maternal LPD, resulting in differential response to breast tumor initiation and susceptibility and may raise discussions about lifelong prevention of breast cancer risk.

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Association between protein undernutrition and diabetes: Molecular implications in the reduction of insulin secretion

dos Reis Araujo,

Alves,

dos Santos

et al. 2023

Rev Endocr Metab Disord

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Maternal low protein diet induces persistent expression changes in metabolic genes in male rats

Cited by 4 publications

References 25 publications

Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

Maternal Low-Protein Diet Deregulates DNA Repair and DNA Replication Pathways in Female Offspring Mammary Gland Leading to Increased Chemically Induced Rat Carcinogenesis in Adulthood

Association between protein undernutrition and diabetes: Molecular implications in the reduction of insulin secretion

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