Maternal micronutrients (folic acid and vitamin B12) and omega 3 fatty acids: Implications for neurodevelopmental risk in the rat offspring

Roy, Suchitra; Kale, Anvita; Dangat, Kamini; Sable, Pratiksha; Kulkarni, Asmita; Joshi, Sadhana

doi:10.1016/j.braindev.2011.01.002

Cited by 60 publications

(42 citation statements)

References 52 publications

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“…Peaks were identified by comparison with standard mixtures of fatty acid methyl esters (Sigma) and by comparison with reference group chromatograms and reported as g/100 gm fatty acids. This method has been extensively published by us earlier [10][11][12][13]. Fatty acids were expressed as g/100 g fatty acids.…”

Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

et al. 2015

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In vegetarian population, vitamin B12 deficiency coexists with suboptimal levels of omega-3 fatty acids. Studies indicate a need for supplementation/fortification of vitamin B12 and omega-3 fatty acids to reduce the risk of brain disorders. We have described the effects of vitamin B12 and omega-3 fatty acid supplementation on brain development in F1 generation animals. The current study investigates the effects of vitamin B12 and omega-3 fatty acids supplementation on brain function and cognition. Pregnant Wistar rats were assigned the following groups: control, vitamin B12 deficient (BD), vitamin B12 deficient + omega-3 fatty acid (BDO), vitamin B12 supplemented (BS), vitamin B12 supplemented + omega-3 fatty acid (BSO). The same diets were continued for two generations. BDO group showed higher (P < 0.05) levels of BDNF (brain derived neurotrophic factor) and DHA (docosahexaenoic acid) in the cortex and hippocampus as compared with the BD group. The cognitive performance was also normalized in this group. BS showed comparable levels of DHA, BDNF (protein and mRNA), and CREB mRNA (cAMP response element-binding protein) to that of control group while Tropomyosin receptor kinase mRNA levels were higher. The combined vitamin B12 and omega-3 fatty acid supplementation further enhanced the levels of DHA (P < 0.05) and BDNF (P < 0.05) in the hippocampus and CREB mRNA (P < 0.01) in the cortex as compared with BS group. The cognitive performance of these animals was higher (P < 0.05) as compared with BS group. Our data indicates the beneficial effects of vitamin B12 and omega-3 fatty acid supplementation across two generations on brain development and function.

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Section: Analysis Of Fatty Acidsmentioning

confidence: 99%

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

et al. 2015

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“…Although ASD is known to be heritable [Neale et al, 2012], environmental conditions including the status of nutritional vitamins (especially photolabile ones) may be involved through vitamin-sensitive genes via epigenetic regulation [Lucock and Leeming, 2013]. Several studies have proposed that excessive FA (PteGlu) intake may be detrimental to neural development [Rogers, 2008;Leeming and Lucock, 2009;Lucock and Leeming, 2013;Choi et al, 2014], and some have provided evidence that it is [Beard et al, 2011;Desoto and Hitlan, 2012;Roy et al, 2012], although other studies report only a protective effect of PteGlu against ASD [Hoyo et al, 2011;Suren et al, 2013]. DeSoto and Hitlan [2012], using a large dataset from the Centers for Disease Control and controlling for health-seeking behaviors and demographics, such as age, poverty and birth order, found that PteGlu supplementation increased the odds of having a child diagnosed with autism by more than 2.5 times compared to mothers who did not supplement.…”

Section: Introductionmentioning

confidence: 99%

Influence of Folic Acid on Neural Connectivity during Dorsal Root Ganglion Neurogenesis

Wiens

DeWitt

Kosar

et al. 2016

Cells Tissues Organs

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The vitamin folic acid (FA) is essential for DNA synthesis, repair and methylation, and for methionine synthesis. Although it is necessary for neural development, recent studies suggest a possible link between excess maternal supplemental FA intake and adverse interferences with single-carbon metabolism and neural development. Insufficient FA early in brain development can lead to failure of the neural tube closure, but the consequences of too much intake have not been fully investigated. Plasma FA concentrations can increase greatly with dietary supplementation. To model the development of neural connectivity, we cultured dorsal root ganglia (DRGs) taken from 8-day-old chick embryos in a range of pteroylmonoglutamate (PteGlu, synthetic supplemental FA) concentrations. DRGs were cultured for 36 h, fixed and immunostained to reveal the locations of neural networks with synaptic vesicles. We found a concentration-dependent relationship with significant reduction in neurite length in PteGlu concentrations from 0.25 to 20 μM. The average total of stained synaptic areas surrounding each cultured DRG was significantly reduced as well. To further characterize the effects, we carried out time-lapse imaging of growth cones at terminals of extending neurites. We found that PteGlu reduced the area-changing activity of the growth cone, hindering its exploratory capabilities, along with a tendency to inhibit overall advancement, thus altering the ability to extend and form synapses. Our results show that PteGlu at 250 nM and higher reduces neurite extension and synapse formation in a dose-dependent manner during neurogenesis, and that its effect is mediated through inhibition of growth cone motility.

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“…Investigating whether one-carbon pathways and epigenetic mechanisms are involved is of increasing interest. In rodent models, providing a maternal diet with excess folic acid and restricted vitamin B 12 (designed to reflect the nutritional situation in much of rural India) is associated with increased maternal oxidative stress, lower level of offspring placental and brain docosahexaenoic acid, and decreased placental global methylation [66]. However, maternal supplementation with PUFAs seems to partially ameliorate the disrupted one-carbon metabolism associated with this diet [67].…”

Section: Polyunsaturated Fatty Acidsmentioning

confidence: 99%

Fetal Origins of Obesity, Cardiovascular Disease, and Type 2 Diabetes

Kruger¹,

Levitt²

2017

The Biology of the First 1,000 Days

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Maternal micronutrients (folic acid and vitamin B12) and omega 3 fatty acids: Implications for neurodevelopmental risk in the rat offspring

Cited by 60 publications

References 52 publications

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

Influence of Folic Acid on Neural Connectivity during Dorsal Root Ganglion Neurogenesis

Fetal Origins of Obesity, Cardiovascular Disease, and Type 2 Diabetes

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Maternal micronutrients (folic acid and vitamin B12) and omega 3 fatty acids: Implications for neurodevelopmental risk in the rat offspring

Cited by 60 publications

References 52 publications

Beneficial effects of omega‐3 fatty acids and vitamin B12 supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

Beneficial effects of omega‐3 fatty acids and vitamin B12 supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

Influence of Folic Acid on Neural Connectivity during Dorsal Root Ganglion Neurogenesis

Fetal Origins of Obesity, Cardiovascular Disease, and Type 2 Diabetes

Contact Info

Product

Resources

About

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats

Beneficial effects of omega‐3 fatty acids and vitamin B₁₂ supplementation on brain docosahexaenoic acid, brain derived neurotrophic factor, and cognitive performance in the second‐generation Wistar rats