Maternal n-3 PUFA deficiency alters uterine artery remodeling and placental epigenome in the mice

Vilasagaram, Srinivas; Molangiri, Archana; Mallepogu, Aswani; Kona, Suryam Reddy; Ibrahim, Ahamed; Duttaroy, Asim K.; Basak, Sanjay

doi:10.1016/j.jnutbio.2021.108784

Cited by 21 publications

(30 citation statements)

References 58 publications

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“…As reported earlier [22], the intake of an n-3 PUFA deficient diet did not influence a change in pre-pregnancy bodyweight in the dam. The cumulative food intake (Kcal) and feed efficiency (%) did not differ over a period of nine-weeks in dams (data not presented).…”

Section: The Maternal N-3 Pufa Deficiency Changes Brain Weight In The...supporting

confidence: 82%

“…NCLAS/IAEC/02/2017). The 21-d offspring brain and breast milk examined in the present investigation were obtained from our recently conducted pre-clinical trial [22]. Weanling female Swiss albino (n=30 per group) mice were kept in pairs, fed on a chow diet for a week, and maintained at 23±3°C, 55% ± 10% relative humidity with ∼12 h dark/light cycle.…”

Section: Methodsmentioning

confidence: 99%

“…Maternal n-3 PUFA deficiency decreased brain DHA content in the offspring [20, 21] and increased the percentage of total n-6 PUFAs in tissue [22]. High dietary n-6 fatty acid compromises brain DHA accretion and contributes to poor neurodevelopment [23].…”

Section: Introductionmentioning

confidence: 99%

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Dietary omega-3 fatty acid deficiency from pre-pregnancy to lactation affects expression of genes involved in neurogenesis of the offspring

Srinivas¹,

Varma²,

Kona³

et al. 2022

Preprint

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Maternal n-3 PUFA (omega-3) deficiency can affect brain development in utero and postnatally. Despite the evidence, the impacts of n-3 PUFA deficiency on the expression of neurogenesis genes in the postnatal brain remained elusive. Since postnatal brain development requires PUFAs via breast milk, we examined the fatty acid composition of breast milk and hippocampal expression of neurogenesis genes in n-3 PUFA deficient 21d mice. In addition, expression of fatty acid desaturases, elongases, signalling receptors of free fatty acids, insulin and leptin, and glucose transporters were measured. Among the genes involved in neurogenesis, the expression of brain-specific tenascin-R (TNR) was downregulated to a greater extent (~31 folds), followed by adenosine A2A receptor (A2AAR), dopamine receptor D2 (DRD2), glial cell line-derived neurotrophic factor (GDNF) expression in the n-3 PUFA deficient hippocampus (p<0.05). Increasing dietary LA to ALA (50:1) elevated ARA to DHA ratio by ~8 folds in the n-3 PUFA deficient breast milk, with an overall increase of total n-6/n-3 PUFAs by ~15:1 (p<0.05) compared to n-3 PUFA sufficient (LA to ALA: 2:1) diet. The n-3 PUFA deficient brain exhibited upregulation of FADS1, FADS2, ELOVL2, ELOVL5, ELOVL6, GPR40, GPR120, LEPR, IGF1 and downregulation of GLUT1, GLUT3, and GLUT4 mRNA expression (p<0.05). Maternal n-3 PUFA deficiency affects the expression of key neurogenesis genes in the offspring with concomitant expression of desaturases and elongases genes suggesting the importance of dietary n-3 PUFA for neurodevelopment.

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Section: The Maternal N-3 Pufa Deficiency Changes Brain Weight In The...supporting

confidence: 82%

Section: Methodsmentioning

confidence: 99%

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Dietary omega-3 fatty acid deficiency from pre-pregnancy to lactation affects expression of genes involved in neurogenesis of the offspring

Srinivas¹,

Varma²,

Kona³

et al. 2022

Preprint

Self Cite

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“…Inadequate n-3 PUFA leads to docosahexaenoic acid (DHA) deficiency that could affect fetal and postnatal neurodevelopment [33], feto-placental changes in epigenetics [34],…”

Section: Introductionmentioning

confidence: 99%

“…Inadequate n-3 PUFA leads to docosahexaenoic acid (DHA) deficiency that could affect fetal and postnatal neurodevelopment [33], feto-placental changes in epigenetics [34], offspring growth and lipogenic capacity [35], and others. PUFA and its metabolites also regulate bone formation and resorption [17].…”

Section: Introductionmentioning

confidence: 99%

Maternal omega-3 fatty acid deficiency affects fetal thermogenic development and postnatal musculoskeletal growth in mice

Srinivas¹,

Molangiri²,

Varma³

et al. 2022

Preprint

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Maternal omega-3 (n-3) polyunsaturated fatty acids (PUFAs) deficiency can affect offspring adiposity and metabolism by modulating lipid and glucose metabolism. However, the impact of n-3 PUFA deficiency on the development of fetal thermogenesis and its consequences is not reported. Using an n-3 PUFA deficient mice, we assessed fetal interscapular brown adipose tissue (iBAT), body fat composition, insulin growth factor-1 (IGF-1), glucose transporters (GLUTs), and expression of lipid storage & metabolic proteins in the offspring. The n-3 PUFA deficiency did not change the pups calorie intake, organ weight, and body weight. However, the offsprings skeletal growth was altered due to excess fat to lean mass, reduced tibia & femur elongation, dysregulated IGF-1 in the mother and pups (p<0.05). Localization of uncoupling protein 1 (UCP1) in iBAT exhibited a reduced expression in the deficient fetus. Further, UCP1, GLUT1, GPR120 were downregulated while FABP3, ADRP, GLUT4 expressions were upregulated in the BAT of the deficient offspring (p<0.05). The deficiency decreased endogenous conversion of the n-3 LCPUFAs from their precursors and upregulated SCD1, FASN, and MFSD2A mRNAs in the liver (p<0.05). An altered musculoskeletal growth in the offspring is associated with impaired browning of the fetal adipose, dysregulated thermogenesis, growth hormone, and expression of glucose and fatty acid metabolic mediators due to maternal n-3 PUFA deficiency. BAT had higher metabolic sensitivity compared to WAT in n-3 PUFA deficiency. Maternal n-3 PUFA intake may prevent excess adiposity by modulating fetal development of thermogenesis and skeletal growth dynamics in the mice offspring.

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Omega-3 Fatty Acids and Ageing Brain

Boga,

Basak

2023

Evidence-Based Functional Foods for Prevention of Age-Related Diseases

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Maternal n-3 PUFA deficiency alters uterine artery remodeling and placental epigenome in the mice

Cited by 21 publications

References 58 publications

Dietary omega-3 fatty acid deficiency from pre-pregnancy to lactation affects expression of genes involved in neurogenesis of the offspring

Dietary omega-3 fatty acid deficiency from pre-pregnancy to lactation affects expression of genes involved in neurogenesis of the offspring

Maternal omega-3 fatty acid deficiency affects fetal thermogenic development and postnatal musculoskeletal growth in mice

Omega-3 Fatty Acids and Ageing Brain

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