Maternal Nanos represses
 hid
 /
 skl
 -dependent apoptosis to maintain the germ line in
 Drosophila
 embryos

Sato, Keitaro; Hayashi, Yoshiki; Ninomiya, Yuichi; Shigenobu, Shuji; Arita, Kayo; Mukai, Masanori; Kobayashi, Satoru

doi:10.1073/pnas.0610052104

Cited by 86 publications

(137 citation statements)

References 66 publications

(87 reference statements)

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“…For example, the mitochondrial large ribosomal RNA and the BTB/POZ protein Germ cell-less (Gcl) are both involved in pole cell formation (Iida and Kobayashi, 1998;Robertson et al, 1999). Likewise, the maternal RNA binding protein Nos is essential to repress mitosis, apoptosis, and somatic differentiation of pole cells (Asaoka-Taguchi et al, 1999;Hayashi et al, 2004;Sato et al, 2007). polar granule component (pgc) RNA is required for pole cell survival (Nakamura et al, 1996;Martinho et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Maternal RNAs encoding transcription factors for germline-specific gene expression in Drosophila embryos

Yatsu

Hayashi²,

Mukai³

et al. 2008

Int. J. Dev. Biol.

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In early Drosophila embryos, germ plasm is localized to the posterior pole region and is partitioned into the germline progenitors, known as pole cells. Germ plasm contains the maternal factors required for germline development. It has been proposed that germline-specific gene expression is initiated by the function of maternal factors that are enriched in the germ plasm. However, such factors have remained elusive. Here, we describe a genome-wide survey of maternal transcripts that encode for transcription factors and are enriched in the germ plasm. We isolated pole cells from blastodermal embryos by fluorescence-activated cell sorting (FACS) and then used these isolated cells in a microarray analysis. Among the 835 genes in the Gene Ontology (GO) category "transcription regulator activity" listed in FlyBase, 68 were found to be predominantly expressed in pole cells as compared to whole embryos. As the early pole cells are known to be transcriptionally quiescent, the listed transcripts are predicted to be maternal in origin. Our in situ hybridization analysis revealed that 27 of the 68 transcripts were enriched in the germ plasm. Among the 27 transcripts, six were found to be required for germline-specific gene expression of vasa and/or nanos by knockdown experiments using RNA interference (RNAi). The identified transcripts encode a transcriptional activator (ovo), components of the transcriptional initiation complexes (Trf2, bip2 and Tif-IA), and the Ccr4-Not complex (CG31716 and l(2)NC136). Our study demonstrates that germ plasm contains maternal transcripts encoding transcriptional regulators for germline-specific gene expression in pole cells.

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Section: Introductionmentioning

confidence: 99%

Maternal RNAs encoding transcription factors for germline-specific gene expression in Drosophila embryos

Yatsu

Hayashi²,

Mukai³

et al. 2008

Int. J. Dev. Biol.

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“…In Drosophila, Nanos forms a complex with another RNA-binding protein, Pumilio, and represses the translation of the hunchback, cyclin B, and hid mRNAs thereby establishing embryonic polarity, mitotic quiescence, and suppression of apoptosis, respectively (8)(9)(10). Three Nanos homologs, Nanos1-3, exist in the mouse, among which Nanos3 and Nanos2 are expressed in the germ cells and are required to protect these cells from undergoing apoptosis during migration and after colonization of the male gonads, respectively (11,12).…”

mentioning

confidence: 99%

NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs

Suzuki

Igarashi

Aisaki

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Nanos is one of the evolutionarily conserved proteins implicated in germ cell development. We have previously shown that NANOS2 plays an important role in both the maintenance and sexual development of germ cells. However, the molecular mechanisms underlying these events have remained elusive. In our present study, we found that NANOS2 localizes to the P-bodies, known centers of RNA degradation that are abundantly accumulated in male gonocytes. We further identified by immunoprecipitation that the components of the CCR4-NOT deadenylation complex are NANOS2-interacting proteins and found that NANOS2 promotes the localization of CNOT proteins to P-bodies in vivo. We also elucidated that the NANOS2/CCR4-NOT complex has deadenylase activity in vitro, and that some of the RNAs implicated in meiosis interact with NANOS2 and are accumulated in its absence. Our current data thus indicate that the expression of these RNA molecules is normally suppressed via a NANOS2-mediated mechanism. We propose from our current findings that NANOS2-interacting RNAs may be recruited to P-bodies and degraded by the enzymes contained therein through NANOS2-mediated deadenylation.germ cells | P-body | meiosis

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“…Manchette appeared in spermatids at step 7 and continued to be observed until step 14. However, the signals for NANOS1 were detected in manchette in spermatids at steps [8][9][10][11]. Flagella started to form in spermatids at step 7 and the NANOS1 signals appeared first in the dense fiber of spermatids at step 12.…”

Section: Immunoelectron Microscopic Localization Of Nanos1 In Non-nuamentioning

confidence: 99%

“…The nanos gene has been extensively studied in Drosophila, and has been shown to play multiple roles in the development and maintenance of the germ line [1][2][3], embryonic patterning [4][5][6][7], migration of the primordial germ cells to the embryonic gonad [1,[8][9][10], and inhibition of apoptosis of germ line cells in embryo [11]. There are three homologous genes, NANOS1, NANOS2 and NANOS3, in mouse [10,12].…”

Section: Introductionmentioning

confidence: 99%

Expression and Localization of NANOS1 in Spermatogenic Cells during Spermatogenesis in Rat

Yokota¹,

Onohara²

2013

CellBio

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Expression and localization of NANOS1 in the spermatogenic cells of rat testis were studied by immunofluorescence and immunoelectron microscopy. Using immunofluorescence techniques, NANOS1 was localized in the cytoplasm and discrete granules of spermatocytes and spermatids. The staining intensity of NANOS1 signal varied depending upon the stage of the cycle of seminiferous epithelium. Double immunofluorescence staining with antibodies against NANOS1 and DDX4 showed that several DDX4-positive compartments of nuage were also stained for NONOS1. Immunoelectron microscopy revealed that the major subcellular localization sites for NANOS1 were the chromatoid body (CB) and satellite body (SB), and the minor sites were the rest of the nuage compartments, including the irregularly-shaped perinuclear granules (ISPG), and intermitochondrial cement (IMC). Non-nuage structures such as mitochondria-associated granules (MAG), granulated body (GB), and reticulated body (RB) were also labeled by theNANOS1 antibody. In addition, NANOS1 was found in the outer dense fibers of flagella of spermatids at steps 12-19, and in the head cap of late spermatids after step 15. These results suggest that NANOS1 is one of the nuage proteins and functions mainly in the CBs as well as in the cytoplasm. NANOS1 may also have additional functions in non-nuage structures such as MAGs, GBs and RBs.

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Maternal Nanos represses hid / skl -dependent apoptosis to maintain the germ line in Drosophila embryos

Cited by 86 publications

References 66 publications

Maternal RNAs encoding transcription factors for germline-specific gene expression in Drosophila embryos

Maternal RNAs encoding transcription factors for germline-specific gene expression in Drosophila embryos

NANOS2 interacts with the CCR4-NOT deadenylation complex and leads to suppression of specific RNAs

Expression and Localization of NANOS1 in Spermatogenic Cells during Spermatogenesis in Rat

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