Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Solves, Pilar; Perales, Alfredo; Moraga, Rosa; Saucedo, E.; Soler, María Rodríguez; Monleón, Javier

doi:10.1159/000084677

Cited by 50 publications

(76 citation statements)

References 45 publications

(24 reference statements)

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“…We read with interest the article by Aroviita and colleagues 1 reporting that the concentration of hematopoietic progenitor cells in cord blood (CB) was higher in male infants, even after adjustment for birth weight. Previously, by use of multivariate analysis, we 2 and others 3,4 did not observe an influence of sex on hematopoietic content of CB. In response to the observations of Aroviita and coworkers and recognizing the importance of infants’ weight on the volume and cell content of CB, 5 we reanalyzed pertinent data for our stored CB units.…”

Section: Characteristics Of Cb Collections By Newborns’ Weight and Smentioning

confidence: 65%

Newborns’ sex and hematopoietic progenitor cell content of cord blood

Solves¹,

Mirabet²,

Perales³

et al. 2005

Transfusion

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We read with interest the article by Aroviita and colleagues 1 reporting that the concentration of hematopoietic progenitor cells in cord blood (CB) was higher in male infants, even after adjustment for birth weight. Previously, by use of multivariate analysis, we 2 and others 3,4 did not observe an influence of sex on hematopoietic content of CB. In response to the observations of Aroviita and coworkers and recognizing the importance of infants' weight on the volume and cell content of CB, 5 we reanalyzed pertinent data for our stored CB units.We reviewed data obtained before freezing for CB collected from 1016 male (49%) and 1046 female (51%) newborns. The median gestational age of male infants did not differ from that of females (40 ± 1.2 weeks vs. 39.7 ± 1.3 weeks). The median birth weight of male infants (3443.7 ± 483.7 g) was significantly greater than that of female infants (3291.9 ± 435.7 g; p < 0.001). CB collections from male infants had fewer total nucleated cells (TNCs; 132.3 × 10 7 ± 46 × 10 7 vs. 125.5 × 10 7 ± 117 × 10 7 ; p < 0.001), but more lymphocytes (36 ± 8% vs. 34.6 ± 7%; p < 0.005) and a higher percentage of CD34+ cells (0.39 ± 0.27% vs. 0.33 ± 0.2%, p < 0.001). There were more colony-forming units (CFUs) in CB collections from male newborns compared to female newborns (137.8 × 10 4 ± 129 × 10 4 vs. 125.5 × 10 4 ± 117 × 10 4 ; p < 0.05). We also analyzed the content of CB collections by newborns' weight and sex. CB collected from female newborns had more TNCs, fewer lymphocytes, and lower percentages of CD34+ cells than from male newborns of comparable birth weight (Table 1). Although the percentage of CD34+ cells was greater for CB collected from male newborns, except for the highest weight category (>4000 g), there was no sex-related difference in the total CD34+ content for the CB (Table 1). In addition, we measured CFUs and found no sex-related difference, except for CB from the highest weight category (>4000 g).

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Section: Characteristics Of Cb Collections By Newborns’ Weight and Smentioning

confidence: 65%

Newborns’ sex and hematopoietic progenitor cell content of cord blood

Solves¹,

Mirabet²,

Perales³

et al. 2005

Transfusion

Self Cite

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“…The HUC is an excellent and unique source of various types of cells (differentiated and nondifferentiated) that can be used for A close cooperation between obstetric services (cell harvesting and acquisition) and the biotechnical laboratory (cell processing and tissue engineering) is needed to set up standards in quantity and quality control, sterility purposes, and sampling techniques. In addition various factors such as influence of type of birth (vaginal vs caesarean), vaginal contamination (meconium, bacteria, and so on), influence of maternal infections, and certainly fetal factors (age, weight, infection, gender, sex, and so on) have to be closely evaluated (15,16).…”

Section: Resultsmentioning

confidence: 99%

Umbilical cord cells as a source of cardiovascular tissue engineering

Breymann¹,

Schmidt

Hoerstrup

2006

Stem Cell Rev

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There is increasing scientific evidence that human umbilical cord cells are a valuable source of adult stem cells that can be used for various implications including regenerative medicine and tissue engineering. The review describes the role of progenitor cells (mesenchymal, endothelial, prenatal) for the use in cardiovascular tissue engineering, i.e., the formation of large vessels and heart valves from umbilical cord cells. Currently used replacements in cardiovascular surgery are made of foreign materials with well known drawbacks such as thrombo-embolic complications, infection, loss of functional and biological properties, and others. Especially in the field of replacements in congenital cardiac defects, there would be a need of materials which have the advantage of optimal biological and mechanical properties. In the case of human umbilical cord cells, autologous cells can be used by minimally invasive procedures. The cells have excellent growth capacities and form a neo-matrix with excellent mechanical properties. For optimal growth and modeling, scaffolds are required with high biocompatibility and biodegradability, which allow cell attachment, ingrowth, and organization. Nutrients and waste must be easily transported and cells should be in entire contact with host's body. Finally, regenerated materials can be fully incorporated and the scaffold is completely replaced. Besides these cell and scaffold requirements, feto-maternal conditions and risk factors concerning deriving stem cells are of major interest. There are still many open questions concerning whether and how maternal conditions such as infection (viral or bacterial) or gestational age of the newborn influence stem cell harvesting and quality. If these cells will be used for the construction of replacement materials, it is clear that very strict criteria and protocols be introduced enabling the promising step from isolated cells to a therapeutic device such as a new heart valve. It is hoped that it will be only a question of time until human umbilical cord cells will be used frequently as the source of cardiovascular tissues among others in the clinical setting of treating congenital heart defects.

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“…Por otro lado, se encontró, que de las variables neonatales, la longitud del cordón fue la única que se correlacionó con el recuento total de células nucleadas y no hubo ninguna otra correlación entre las demás variables maternas o neonatales analizadas como vía del parto, peso materno, edad gestacional, peso de placenta, peso del bebé datos que contrastan en cuanto a que el peso del neonato al momento del nacimiento es el principal factor neonatal que afecta el Numero de CD34+ y Recuento de células nucleadas (7)(8)(9)(10)22,(26)(27)(28)(29)(30)(31). Llama la atención el hecho que se hubiera encontrado correlación solamente entre la longitud del cordón umbilical y el recuento total de células nucleadas y no también con el recuento de células CD34+, teniendo en cuenta, y según los resultados del presente estudio, la existencia de correlación entre estos dos marcadores de calidad, inquietudes que valen la pena tener en cuenta para próximas investigaciones.…”

Section: Discussionunclassified

Correlación entre características físicas neonatales y maternas con el recuento total de células nucleadas y de células CD34+ por microlitro en sangre de cordón umbilical

et al. 2015

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ResumenIntroducción: La sangre de cordón umbilical (SCU) ha sido reconocida como una fuente de células madre hematopoyéticas. Múltiples estudios han sido realizados con el propósito de determinar variables maternas y neonatales que afecten el volumen, el recuento total de células nucleadas y de células CD34+. El presente estudio correlaciona variables maternas y neonatales con el recuento total de células nucleadas y de CD34+ medidas en µL (microlitro). Materiales y Métodos:Estudio correlacional en el que se analizaron 50 muestras de sangre de cordón umbilical de gestantes atendidas en dos IPS, una de Bogotá y otra de Ubaté, (Cundinamarca), durante un período de 7 meses del año 2013. La selección de estas muestras fue basada en un muestreo no probabilístico. Se calculó el coeficiente de correlación de Pearson con su respectiva significancia estadística entre las variables cuantitativas de la madre y del neonato, y el volumen, el recuento de células CD34+ y el recuento total de células nucleadas por µL. Resultados:Se encontró correlación positiva entre la longitud del cordón y el recuento total de células nucleadas. Así mismo entre el volumen inicial de la muestra y el recuento total de células nucleadas y el recuento de células CD34+ en µL y en mL (mililitro). Discusión:Múltiples estudios se han desarrollado entorno a la búsqueda de variables maternas y neonatales que afectan la calidad de la muestra. En Colombia no se habían descrito las correlaciones encontradas en el presente estudio. Es por ello, que el desarrollo de nuevos estudios con diseños analíticos será enriquecedor. Palabras claves: Células madre, antígeno CD34+, trasplante de células madre de sangre de cordón umbilical (SCU), neonatal, materno, factores de riesgo. CORRELATION BETWEEN NEONATAL AND MATERNAL PHYSICAL CHARACTERISTICS WITH TOTAL NUCLEATED CELL COUNT AND CELL COUNT PER MICROLITER CD34+ IN CORD BLOOD AbstractBackground: The cord blood has been recognized as a source of hematopoietic stem. Multiple studies have been conducted in order to determine maternal and neonatal variables that affect the volume, total nucleated cell count and CD34+ cells. This study correlated maternal and neonatal variables with the total nucleated cell count and CD34+ cells measured by µL. Materials and methods: Correlational study in which 50 samples of umbilical cord blood were analyzed of pregnant women at two health institutions, one at Bogotá and the other one at Ubaté, Cundinamarca, for a period of seven months of 2013. The selection of these samples was based on a non-probability sample. The Pearson correlation coefficient was calculated with their respective statistical significance between quantitative variables of mother and newborn, and the volume, the count of CD34+ cells and the total nucleated cell count measured by µL. Results:Positive correlation between the length of the umbilical cord and the total nucleated cell count was found. Similarly, correlation between total nucleated cell count with the initial volume and count of CD34+ cells in µL and m...

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Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units

Cited by 50 publications

References 45 publications

Newborns’ sex and hematopoietic progenitor cell content of cord blood

Newborns’ sex and hematopoietic progenitor cell content of cord blood

Umbilical cord cells as a source of cardiovascular tissue engineering

Correlación entre características físicas neonatales y maternas con el recuento total de células nucleadas y de células CD34+ por microlitro en sangre de cordón umbilical

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