Maternal Oct-4 is a potential key regulator of the developmental competence of mouse oocytes

Zuccotti, Maurizio; Merico, Valeria; Sacchi, Lucia; Bellone, Michele; Brink, Thore C.; Bellazzi, Riccardo; Stefanelli, Mario; Redi, Carlo Alberto; Garagna, Silvia; Adjaye, James

doi:10.1186/1471-213x-8-97

Cited by 72 publications

(54 citation statements)

References 63 publications

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“…A total of 123 antral oocytes isolated from the ovaries of four females were analysed by immunocytochemistry, 49 (39.8%) and 74 (60.2%) of them owned an NSN-or SN-type of chromatin organisation, respectively, confirming frequencies previously reported [24,27]. This analysis, demonstrated the presence of the acetylated form of the three histones in all the oocytes observed (AcH3: 43 oocytes analysed; AcH2B: 37; AcH4: 43).…”

Section: Resultssupporting

confidence: 80%

“…When the expression of one of the 27 maternal-effect factors identified so far is altered, mouse embryos arrest development, mostly at the 2-cell stage (for a review see [13]). We have recently shown that one of the possible causes of the developmental incompetence of MII NSN oocytes is the down-regulation of two maternaleffect factors, OCT4 and STELLA [27,28], required for development beyond the 2-cell stage [8,20].…”

Section: Introductionmentioning

confidence: 99%

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Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Zuccotti

Bellone

Longo

et al. 2011

J Assist Reprod Genet

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Purpose The final stages of antral mouse oocytes maturation are characterised by the transition from transcriptionally active NSN to inactive SN oocytes. Here, we studied the profile of histone acetylation changes occurring during the NSN-to-SN transition. Methods During the NSN-to-SN transition, oocytes were classified based on their chromatin organisation and the immunocytochemical profile of histones H2B, H3 and H4 acetylation was analysed. Results We described four patterns of immunostaining common to the three acetylated histones, corresponding to stages of progressive localisation: From a diffused distribution in NSN oocytes to the association with constitutive heterochromatin and then to the nucleolar surface region in SN oocytes. Conclusions The maintenance of a favourable transcriptional epigenetic context, in the heterochromatin of transcriptionally silent SN oocytes, might be related to the early stages of development when transcripts from these heterochromatic regions are functional to preimplantation progression.Keywords Mouse . Antral oocytes . Chromatin organisation . Histone acetylation Capsule In late antral mouse oocytes, chromatin condenses around the nucleolus, becomes transcriptionally silent, but maintains transcriptionally permissive histone acetylation profiles, likely functional to development.

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Section: Resultssupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Zuccotti

Bellone

Longo

et al. 2011

J Assist Reprod Genet

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“…From recent investigations, it has also emerged that Sox2 is one of the transcription factors underlying the derivation of induced pluripotent stem cells [2]. Maternal Sox2 RNA and protein, as for Oct4 [3], are present in fertilized oocytes until the two-cell stage. Zygotic Sox2 RNA, during embryogenesis, is expressed in the morula at 2.5 days postcoitum (dpc), inner cell mass of the blastocyst from 3.5 dpc and, at a later stage, in epiblast cells [4].…”

Section: Introductionmentioning

confidence: 99%

Essential Role of Sox2 for the Establishment and Maintenance of the Germ Cell Line

et al. 2013

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Sox2 is a pluripotency-conferring gene expressed in primordial germ cells (PGCs) and postnatal oocytes, but the role it plays during germ cell development and early embryogenesis is unknown. Since Sox2 ablation causes early embryonic lethality shortly after blastocyst implantation, we generated mice bearing Sox2-conditional deletion in germ cells at different stages of their development through the Cre/loxP recombination system. Embryos lacking Sox2 in PGCs show a dramatic decrease of germ cell numbers at the time of their specification. At later stages, we found that Sox2 is strictly required for PGC proliferation. On the contrary, Sox2 deletion in meiotic oocytes does not impair postnatal oogenesis and early embryogenesis, indicating that it is not essential for oocyte maturation or for zygotic development. We also show that Sox2 regulates Kit expression in PGCs and binds to discrete transcriptional regulatory sequences of this gene, which is known to be important for PGCs survival and proliferation. Sox2 also stimulates the expression of Zfp148, which is required for normal development of fetal germ cells, and Rif1, a potential regulator of PGC pluripotency.

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“…With a similar aim, MII developmentally competent oocytes (MII SN ) were compared with oocytes that cease development at the 2-cell stage (MII NSN ) , Zuccotti et al, 2008. The GO enrichment analysis performed with DAVID and the networks obtained using IPA allowed the identification of gene expression networks involved into the regulation of biochemical pathways representative of the adverse biological status of MII NSN oocytes.…”

Section: Applications To -Omics Analysis In Oogenesis and Early Embrymentioning

confidence: 99%

Knowledge-based bioinformatics for the study of mammalian oocytes

et al. 2012

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Bioinformatics tools have been recently applied to study the differentiation of the mammalian oocyte during folliculogenesis. In this review, we will summarize our knowledge of 1) the use of biological databases for the extraction of relevant information, 2) bioinformatics methods for knowledge extraction and representation, 3) the application of these methods to the study of mammalian oocyte differentiation and 4) state-of the-art prediction approaches for the assessment and estimation of the cell differentiation status.

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Maternal Oct-4 is a potential key regulator of the developmental competence of mouse oocytes

Cited by 72 publications

References 63 publications

Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Fully-mature antral mouse oocytes are transcriptionally silent but their heterochromatin maintains a transcriptional permissive histone acetylation profile

Essential Role of Sox2 for the Establishment and Maintenance of the Germ Cell Line

Knowledge-based bioinformatics for the study of mammalian oocytes

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