Maternal periodontitis decreases plasma membrane GLUT4 content in skeletal muscle of adult offspring

Mattera, Maria Sara de Lima Coutinho; Chiba, Fernando Yamamoto; Mota, Mércia Melo de Almeida; Pereira, Renato Felipe; Ervolino, Edílson; Chaves‐Neto, Antônio Hernandes; Salzedas, Leda Maria Pescinini; Scaramele, Natália Francisco; Antoniali, Cristina; Okamoto, Maristela Mitiko; Machado, Ubiratan Fabres; Sumida, Dóris Hissako

doi:10.1016/j.lfs.2016.02.010

Cited by 14 publications

(23 citation statements)

References 52 publications

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“…The association demonstrated between maternal periodontal disease and LBW (Table ) corroborates some previous studies showing that maternal periodontal disease promotes adverse pregnancy outcomes, such as LBW and premature birth . Notwithstanding, other studies have failed to find effects of maternal periodontal disease on birth weights of offspring .…”

Section: Discussionsupporting

confidence: 88%

“…Further, the presence of bacterial products in the fetoplacental unit can trigger the local release of biochemical mediators or pro‐inflammatory cytokines such as TNF‐α, IL‐1, IL‐6, and prostaglandin E2 (PGE2) . We have previously observed that maternal periodontal disease promotes increased plasma concentrations of TNF‐α in adult rats . In this study, we observed an increase in TNF‐α content in the GM of the PED‐o group (Fig.…”

Section: Discussionsupporting

confidence: 51%

“…Our studies have demonstrated that the adult offspring of rats with periodontal disease show an increase in plasma levels of TNF‐α . This cytokine can activate c‐Jun N‐terminal kinases (JNK), IκB kinase β/nuclear factor‐kappa B (IKKβ/NF‐κB) and extracellular signal‐regulated protein (ERK: ERK1 and ERK2) intracellular pathways that are involved in insulin resistance .…”

mentioning

confidence: 96%

“…Our group has previously shown that maternal periodontal disease in rats results in LBW, insulin resistance, increased TNF‐α levels, changes in Akt serine phosphorylation status, and changes in the translocation index and GLUT4 content of the gastrocnemius muscle (GM) in adult offspring …”

mentioning

confidence: 99%

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Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring

Mattera

Chiba²,

Lopes

et al. 2019

Journal of Periodontology

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Background Maternal periodontal disease leads to low birth weight (LBW), insulin resistance (IR), increased TNF‐α levels, and alterations in insulin signaling in adult offspring. TNF‐α has been associated with the stimulation of IKKβ/NF‐κB, resulting in the decreased expression of GLUT4. Another mechanism that may be involved in decreasing GLUT4 expression is DNA methylation. This study aimed to evaluate in the adult offspring of rats with periodontal disease: IR, inflammatory pathways, DNA methylation, and expression of GLUT4. Methods Female Wistar rats were distributed into control and experimental periodontal disease groups. Seven days after induction of periodontal disease, both groups were mated with healthy male rats. After weaning, male offspring were distributed into control offspring (CN‐o) and periodontal disease offspring (PED‐o) groups. Body weights were measured from 0–75 days of age. At day 75, the following were measured in the offspring: IR (HOMA‐IR index); TNF‐α and NF‐κBp65 content in the gastrocnemius muscle (GM) by western blotting; IKKα/β, JNK, ERK 1/2, NF‐κBp65, and NF‐κBp50 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real‐time PCR(qAMP); and expression of GLUT4 mRNA in the GM by real‐time PCR. Results LBW, IR, increases in TNF‐α, IKKα/β, ERK 1/2, NF‐κBp65, and NF‐κBp50 decreased expression of GLUT4 mRNA were observed in the PED‐o rats. No differences were identified in JNK phosphorylation status and DNA methylation in the evaluated regions of the GLUT4‐encoding gene Slc2a4. Conclusion Maternal periodontal disease causes LBW, IR, activation of inflammatory pathways, and decreased GLUT4 expression in the GM of adult offspring.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 96%

mentioning

confidence: 99%

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Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring

Mattera

Chiba²,

Lopes

et al. 2019

Journal of Periodontology

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“…Studies have shown the association amongst maternal periodontal disease (PD) and low birthweight (Mattera et al . ), preterm birth and intrauterine growth restriction (Siqueira et al . ).…”

Section: Introductionmentioning

confidence: 99%

Maternal apical periodontitis is associated with insulin resistance in adult offspring

et al. 2019

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Aim To investigate the plasma concentrations of glucose, insulin and tumour necrosis factor‐α (TNF‐α) of rats with maternal apical periodontitis (AP) and to explore the effect of maternal inflammation on the initial steps of insulin signalling and the inflammatory pathway in the gastrocnemius muscle (GM) and periepididymal white adipose tissue (pWAT) of adult offspring. Methodology Fifteen female Wistar rats were distributed into a control group (CN), a group with 1 tooth with AP (1AP) and a group with 4 teeth with AP (4AP). Thirty days following induction of AP, female rats from all groups were mated with healthy male rats. When male offspring reached 75 days of age, plasma concentrations of glucose, insulin and TNF‐α were quantified. Insulin resistance was evaluated by the homoeostasis model assessment of insulin resistance (HOMA‐IR) index. Phosphorylation status of pp185 tyrosine, insulin receptor substrate 1 (IRS‐1) serine, IκB kinase α/β (IKKα/β) and c‐Jun N‐terminal kinase (JNK) in the GM and pWAT were measured by Western blot. Analysis of variance was performed, followed by the Tukey's post hoc test. P values <0.05 were considered to be statistically significant. Results Maternal AP promoted insulin resistance, impaired the initial steps of insulin signalling, significantly increased plasma concentrations of insulin (P < 0.001) and TNF‐α (P < 0.05), and enhanced IKKα/β phosphorylation in the GM and pWAT (P < 0.05) of adult offspring. However, maternal AP did not affect fasting glycaemia and JNK phosphorylation in the GM and pWAT of adult offspring. Conclusions Maternal AP was associated with insulin resistance in adult offspring through alterations in insulin signalling and inflammation pathways. The study provides information on the impact of maternal AP on the development of metabolic alterations such as insulin resistance in adult offspring and reinforces the importance of preventing maternal AP in order to maintain the general health of offspring.

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Effect of maternal true combined endodontic‐periodontal lesion on insulin and inflammatory pathway in adult offspring

Belardi

Mattera

Tsosura

et al. 2022

Journal of Periodontology

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Background: Maternal periodontal disease (PED) and apical periodontitis (AP) are associated insulin resistance (IR), increased tumor necrosis factor-α (TNFα) levels, and alterations in insulin signaling (IS) in the gastrocnemius muscle (GM) of adult offspring. TNF-α stimulates I kappa B kinase (IKK) and c-Jun Nterminal protein kinase (JNK), resulting in IS attenuation. However, studies that investigated the maternal true endodontic-periodontal lesion (EPL) in offspring are scarce, and in this case, the impact could be even higher. This study aimed to evaluate the effects of EPL on the IR, IS, and inflammatory pathways on the offspring GM.Methods: Female Wistar rats were distributed into control, AP, PED, and EPL groups. After 30 days of oral inflammation induction, rats from all groups were allowed to mate with healthy rats. The body weight of the offspring was assessed from birth to 75 days of age. After 75 days, the following measurements were performed: glycemia, insulinemia, IR, TNF-α content, and IKKα/β, JNK, pp185 (Tyr), and IRS-1 (Ser) phosphorylation status in the GM. Results: Maternal PED and EPL were associated with low birth weights. All

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Maternal periodontitis decreases plasma membrane GLUT4 content in skeletal muscle of adult offspring

Abstract: Maternal periodontal disease causes LBW, insulin resistance, and alterations in the final stage of insulin signaling in the GSM of adult offspring.

Cited by 14 publications

References 52 publications

Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring

Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring

Maternal apical periodontitis is associated with insulin resistance in adult offspring

Effect of maternal true combined endodontic‐periodontal lesion on insulin and inflammatory pathway in adult offspring

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