Maternal serum thrombospondin-1 is significantly altered in cases with established preeclampsia

Ulu, İpek; Çekmez, Yasemin; Köpük, Şule Yıldırım; Özer, Nida; Yoğurtçuoğlu, Eser Evrim; Anğın, Pınar; Grover, Kiran

doi:10.1080/14767058.2018.1441279

Cited by 8 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a minor level, however, we detected a weak positive value with TSP-1 (about 0.5 value with the 10 ng of TSP-1; i.e., about 5% level of sFLT1). TSP-1 was reported to be about 207 ng/ml in the serum of normal pregnancy, and 11%-decrease in the severe PE (non PE: 207 ng/ml serum; severe PE: about 183 ng/ml) 40 . Since each serum sample for the sFLT1 assay contains basal level of TSP-1, and the difference in the TSP-1 between non PE and severe PE is not so high, we conclude that the sFLT1 values detected with this HB-ELISA are not significantly modulated with the TSP-1.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A simple detection method for the serum sFLT1 protein in preeclampsia

Shibuya

Matsui

Sasagawa

et al. 2021

Sci Rep

View full text Add to dashboard Cite

In normal pregnancy, the soluble form of FMS-like tyrosine kinase-1 (sFLT1)/ vascular endothelial growth factor receptor-1 (sVEGFR-1), a VEGF-trapping protein, is expressed in trophoblasts of the placenta, suggesting that it plays an important role in the physiological barrier between fetal and maternal angiogenesis, when stimulated with VEGF-A. In pathological conditions such as preeclampsia (PE), sFLT1 protein is abnormally overexpressed in trophoblasts and secreted into the serum, which could cause hypertension and proteinuria on the maternal side and growth retardation on the fetal side. Detection of an abnormal increase in serum sFLT1 during the early to middle stages of PE is essential for proper initiation of medical care. To carry out this screening for sFLT1, we developed an easier and relatively low-cost sandwich-type ELISA method using a single mixture of human serum sample with an anti-FLT1 antibody and heparin-beads, namely heparin-beads-coupled ELISA (HB-ELISA). This method takes only about 2 h, and the sFLT1 values were similar levels with commercially available recent ELISA kits: the serum sFLT1 protein was approximately 4.3-fold increased in severe PE compared with those in normal pregnancy.

show abstract

Section: Resultsmentioning

confidence: 99%

“…To further validate the sFLT1 HB-ELISA, we tested several human proteins with the HB-ELISA. Non-heparin-binding type: Insulin and Epidermal growth factor (EGF) 39 ; and heparin-binding type: Thrompospondin-1 (TSP-1) 40 , Heparin-binding EGF-like growth factor (HB-EGF) 39 and Antithrombin III 41 were examined. As indicated in Fig.…”

Section: Resultsmentioning

confidence: 99%

A simple detection method for the serum sFLT1 protein in preeclampsia

Shibuya

Matsui

Sasagawa

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…S100A8/A9 have been analyzed in breast milk; concentrations were significantly higher after term delivery, compared with PTD, and after vaginal delivery, compared with cesarean section [55]. Thrombospondin-1 is expressed in the placenta and has previously been reported in cases of small for gestational age (SGA) pregnancies and preeclampsia [56,57].…”

Section: Discussionmentioning

confidence: 99%

Protein Concentrations of Thrombospondin-1, MIP-1β, and S100A8 Suggest the Reflection of a Pregnancy Clock in Mid-Trimester Amniotic Fluid

et al. 2020

View full text Add to dashboard Cite

The development of immunoassays enables more sophisticated studies of the associations between protein concentrations and pregnancy outcomes, allowing early biomarker identification that can improve neonatal outcomes. The aim of this study was to explore associations between selected mid-trimester amniotic fluid proteins and (1) overall gestational duration and (2) spontaneous preterm delivery. A prospective cohort study, including women undergoing mid-trimester transabdominal genetic amniocentesis, was performed in Gothenburg, Sweden, 2008–2016 (n = 1072). A panel of 27 proteins related to inflammation was analyzed using Meso-Scale multiplex technology. Concentrations were adjusted for gestational age at sampling, experimental factors, year of sampling, and covariates (maternal age at sampling, parity (nulliparous/multiparous), smoking at first prenatal visit, and in vitro fertilization). Cox regression analysis of the entire cohort was performed to explore possible associations between protein concentrations and gestational duration. This was followed by Cox regression analysis censored at 259 days or longer, to investigate whether associations were detectable in women with spontaneous preterm delivery (n = 47). Finally, linear regression models were performed to analyze associations between protein concentrations and gestational duration in women with spontaneous onset of labor at term (n = 784). HMG-1, IGFBP-1, IL-18, MIP-1α, MIP-1β, S100A8, and thrombospondin-1 were significantly associated with gestational duration at term, but not preterm. Increased concentrations of thrombospondin-1, MIP-1β, and S100A8, respectively, were significantly associated with decreased gestational duration after the Holm-Bonferroni correction in women with spontaneous onset of labor at term. This adds to the concept of a pregnancy clock, where our findings suggest that such a clock is also reflected in the amniotic fluid at early mid-trimester, but further research is needed to confirm this.

show abstract

“…При морфологічному дослідженні виявляються крововиливи у плаценті. Гостра ПД може виникати в будь-який термін вагітності та під час пологів, та є причиною гострої гіпоксії плода й загибелі плоду [12].…”

unclassified

“…Важливим показником антикоагулянтного потенціалу синцитіотрофобласту є тромбомодулін, який експресується із зруйнованих клітин ендотелію та трофобласта, може зворотньо зв'язуватися з тромбіном, утворивши комплекс тромбомодулінтромбін. Цей комплекс активує природний антикоагулянт циркуляції протеїн С. Результати досліджень свідчать про активацію комплексу тромбомодулін-тромбін у пацієнток з хронічною фетоплацентарною недостатністю [12,28,31,33].…”

unclassified

Prediction and Early Diagnosis of Placental Dysfunction (Literature Review)

Borzenko¹

2020

Ukr. ž. med. bìol. sportu

View full text Add to dashboard Cite

Maternal serum thrombospondin-1 is significantly altered in cases with established preeclampsia

Cited by 8 publications

References 19 publications

A simple detection method for the serum sFLT1 protein in preeclampsia

A simple detection method for the serum sFLT1 protein in preeclampsia

Protein Concentrations of Thrombospondin-1, MIP-1β, and S100A8 Suggest the Reflection of a Pregnancy Clock in Mid-Trimester Amniotic Fluid

Prediction and Early Diagnosis of Placental Dysfunction (Literature Review)

Contact Info

Product

Resources

About