Maternal treatment with fluoxetine promotes testicular alteration in male rat pups

Ramos, Aline Camargo; Santos, Alice Hartmann dos; Silveira, Kennia Moura; Kiss, Ana Carolina Inhasz; Mesquita, Suzana de Fátima Paccola; Gerardin, Daniela Cristina Ceccatto

doi:10.1071/rd14199

Cited by 10 publications

(7 citation statements)

References 59 publications

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“…Another study also confirmed the genotoxic Histomorphometric analyses in the testicular tissue showed significant decrease in epithelial height and diameter of SNTs in mice receiving CeO 2 NPs comparing the control group. These alterations could be related to the decrease in the number of Sertoli cells because there is a direct correlation between the number of Sertoli cells and epithelial height and diameter of SNTs (36,37). In the present study, the number of Sertoli cells was not evaluated.…”

Section: Discussionmentioning

confidence: 79%

Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

Hosseinalipour¹,

Karimipour²,

Ahmadi³

2021

IJRM

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Background: Cerium oxide nanoparticles (CeO2 NPs) as an important nanomaterial have a wide range of applications in many fields and human beings’ exposure to this nanomaterial is unavoidable. The effects of CeO2 NPs on the male reproductive system are controversial. Objective: To determine the effects of the administration of CeO2 NPs on the testis tissue, sperm parameters, and in vitro fertilization (IVF) in mice. Materials and Methods: Twenty-four male mice were divided into three groups (n = 8/each): one control and two experimental groups receiving CeO2 NPs at doses of 50 and 100 mg/kg body weight, respectively, for 35 days. At the end of the experiment, the diameter of seminiferous tubules (SNTs), epithelial height of SNTs, spermiogenesis index in testes, sperm parameters (count, motility, viability, and morphology), sperm chromatin condensation, DNA integrity, and IVF assays were analyzed. Results: Histological results showed that the tubular diameter, the epithelial height of the SNTs, and the spermiogenesis index were significantly decreased in the experimental groups receiving CeO2 NPs. All sperm parameters in the experimental groups were significantly reduced and, additionally, the percentages of immature sperms and sperms with DNA damage were significantly increased in groups treated with CeO2 NPs compared to the control. Furthermore, the rates of IVF and in vitro embryo development were decreased. Conclusion: Collectively, the current study showed that oral administration of CeO2 NPs in mice had detrimental effects on the male reproductive system through inducing testicular tissue alterations, decreasing sperm parameters quality, and also diminishing the IVF rate and in vitro embryonic development. Key words: Cerium oxide, Testis, Sperm, Fertilization, Mice.

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Section: Discussionmentioning

confidence: 79%

Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

Hosseinalipour¹,

Karimipour²,

Ahmadi³

2021

IJRM

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“…Although an increase in kidney weight was observed, which could be associated with damage to this organ, Perinatal exposure to SSRI can increase 5-HT availability in the testes and epididymis of offspring during the critical period of development, resulting in downregulation of 5-HT receptors in testicular and epididymal cells. These modifications during the development of the testis and epididymis can cause persistent changes in sperm quality and/or quantity (Ramos et al, 2016). Studies have revealed that a local serotonergic system is found in rat caput epididymis including the expression of 5-HT biosynthetic enzymes, 5-HT receptors, and serotonin transporters (Jiménez-Trejo et al, 2007), and disturbances in this serotonergic system have been associated with changes in sperm count and motility as well as ejaculatory alterations (Gonzales et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Lactational exposure to venlafaxine provokes late repercussions on reproductive parameters in male rat offspring

Moreira

Manoel

Inácio

et al. 2022

J of Applied Toxicology

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Exposure to selective serotonin reuptake inhibitors can affect hormone-dependent processes, such as the brain sexual differentiation. Because the use of these antidepressants cause concern during lactation, we evaluated the possible effects of venlafaxine on lactational exposure and its late repercussions on reproductive parameters in male rats. Lactating rats were exposed to venlafaxine (3.85, 7.7, or 15.4 mg/kg/ body weight; gavage), from lactational day 1 to 20. Venlafaxine and Odesmethylvenlafaxine residues were found in all milk samples of dams treated, demonstrating the lactational transfer of this antidepressant to the offspring. Although the maternal behavior was normal, the dams presented an increase in urea and uric acid levels in the groups treated with 7.7 and 15.4, respectively, as well as a spleen weight increased in the 3.85 and 15.4 groups. The male offspring showed a decrease in play behavior parameters in the intermediate dose group. Sperm analysis indicated a reduction in sperm motility in all treated groups. The androgen receptor expression in the hypothalamus was decreased in the highest dose group, although the sexual behavior had not been affected. In conclusion, venlafaxine was transferred through breast milk and promoted changes in play behavior, sperm quality, and hypothalamic androgen receptor (AR) content, which may indicate an incomplete masculinization of the brain of male offspring.

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“…Thus the drugs that enhance or inhibit serotonin activity may have implications on reproduction. The use of antidepressants such as selective serotonin reuptake inhibitors (SSRI) induce negative reproductive effects in adult men [ 59 ], in woman during pregnancy [ 60 ], and male exposed in utero [ 61 ]. SSRI and are widely prescribed for children and adolescents, although data regarding their safety are limited [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat

Ayala¹,

Velázquez²,

Mendoza³

et al. 2015

Reprod Biol Endocrinol

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BackgroundInnervation of the hypothalamus and median eminence arise from the dorsal and medial raphe nuclei (DRN and MRN, respectively). The hypothalamus regulates the secretion of gonadotropins, which in turn regulate the reproductive function of males and females. However, it is not known the role of raphe nuclei in male reproductive function. Our goal was to investigate the role of the DRN and MRN in the regulation of the testicular function and secretion of gonadotropins in prepubertal rats.MethodsDihydroxytryptamine (5,6-DHT) in ascorbic acid was used to chemically lesion the DRN or MRN. Rats were treated at 30 days-of-age and sacrificed at 45 or 65 days-of-age. Sham-treated controls were injected with ascorbic acid only. Negative controls were untreated rats. The damage induced by the 5,6-DHT was monitored in coronal serial sections of DRN and MRN; only the animals in which lesion of the DRN or MRN was detected were included in this study. As output parameters, we measured the concentrations of noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in the anterior (AH) and medial (MH) hypothalamus by high performance liquid chromatography (HPLC); whereas, circulating concentrations of gonadotropins and sexual steroids were measured by radioimmunoassay. Seminiferous epithelium and sperm quality were also evaluated.ResultsLesion of DRN or MRN does not induced changes in concentrations of LH, progesterone, and testosterone. Compared with the control group, the sham or lesion of the DRN or MRN did not modify noradrenaline or dopamine concentrations in the AH and MH at 45 or 65 days of age. Meanwhile, serotonin concentrations decreased significantly in lesioned rats. Lesion of DRN induced significantly lower concentrations of FSH regardless of age; similar lesion in the MRN had no impact on FSH levels. Sperm concentration and motility were significantly decreased in the same animals. The lesion of the MRN does not induced changes in the seminiferous epithelium or gonadotropin levels. Our results suggest that raphe nuclei regulate differentially the male reproductive functions.ConclusionsThe DRN but not the MRN regulates the secretion of gonadotropins and testicular function.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-015-0130-0) contains supplementary material, which is available to authorized users.

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Maternal treatment with fluoxetine promotes testicular alteration in male rat pups

Cited by 10 publications

References 59 publications

Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

Lactational exposure to venlafaxine provokes late repercussions on reproductive parameters in male rat offspring

Dorsal and medial raphe nuclei participate differentially in reproductive functions of the male rat

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