MATH-Domain Family Shows Response toward Abiotic Stress in Arabidopsis and Rice

Abstract

Response to stress represents a highly complex mechanism in plants involving a plethora of genes and gene families. It has been established that plants use some common set of genes and gene families for both biotic and abiotic stress responses leading to cross-talk phenomena. One such family, Meprin And TRAF Homology (MATH) domain containing protein (MDCP), has been known to be involved in biotic stress response. In this study, we present genome-wide identification of various members of MDCP family from both Arabidopsis and rice. A large number of members identified in Arabidopsis and rice indicate toward an expansion and diversification of MDCP family in both the species. Chromosomal localization of MDCP genes in Arabidopsis and rice reveals their presence in a few specific clusters on various chromosomes such as, chromosome III in Arabidopsis and chromosome X in rice. For the functional analysis of MDCP genes, we used information from publicly available data for plant growth and development as well as biotic stresses and found differential expression of various members of the family. Further, we narrowed down 11 potential candidate genes in rice which showed high expression in various tissues and development stages as well as biotic stress conditions. The expression analysis of these 11 genes in rice using qRT-PCR under drought and salinity stress identified OsM4 and OsMB11 to be highly expressed in both the stress conditions. Taken together, our data indicates that OsM4 and OsMB11 can be used as potential candidates for generating stress resilient crops.

Contribution of CaBPM4, a BTB Domain–Containing Gene, to the Response of Pepper to Phytophthora capsici Infection and Abiotic Stresses

“…P. capsici was also revealed to downregulate CaBPM4 expression, which was contrary to the results of previous research that found rice genes including OsMB9, OsMB10, and OsMB11 show upregulated expression under infection [28]. Few plant species, including Arabidopsis, have shown trends similar to our result.…”

“…In Arabidopsis, BPM1, BPM4, and BPM5 are reported to be upregulated under salt and drought stresses [31]. Kushwaha et al (2016) [28] also noted that OsMB10 and OsMB11, which encode BPM proteins in rice, showed apparent increases in expression level under salinity and drought stress. Inhibitory effects of exogenous substances, especially ABA, on CaBPM4 expression were observed in the current study, which is unsurprising.…”

“…The BTB domain-containing protein gene family of tomato was also well-known to be related closely to abiotic stresses [26].Many BTB/POZ proteins contain a secondary protein domain, such as a meprin and TRAF (tumor necrosis factor receptor associated factor) homology (MATH) domain, a tumor necrosis factor receptor-associated element [27]. A previous study suggested that MATH domain-containing proteins positively regulate defense responses to abiotic stresses in Arabidopsis and rice [28]. Different functional roles have been identified for BTB proteins, including transcription repression, cytoskeleton regulation, and protein degradation, in which the amino-terminal MATH domain is responsible for substrate specificity [21].…”

Genetic dissection of heat-responsive physiological traits to improve adaptation and increase yield potential in soft winter wheat

“…This protein was found to be over expressed upon high temperature stress in bread wheat [61]. We found several other MTAs whose annotations suggest different roles including abiotic stress response (BTB/POZ and MATH domain-containing protein 2), senescence (Maintenance of telomere capping protein 2, Protein Phosphatase 2C) and salinity stress (Peptidase M50 family protein) [62][63][64].…”

Genome-wide transcript analysis of inflorescence development in wheat

“…Ta.22183.1.s1_at, which is similar to AtBRCA1, an ortholog of the human breast cancer susceptibility gene 1 that contains one N-terminal RING finger domain, and two C-terminal BRCT and a CBP interaction domain with DNA repair and cell cycle control functions (Lafarge and Montané 2003), is also expressed mainly in the early stages of inflorescence development. Ta.6830.1.s1_at is an ortholog to MATH-BTB domain proteins that directly interact with, and target, proteasomal degradation of the class I homeobox-leucine zipper transcription factor in Arabidopsis and are also highly expressed in early stage wheat inflorescence development (Kushwaha et al 2016;Weber et al 2005).…”