Mathematical Model of L-Lactic Acid Fermentation in a RDC Coupled With Product Separation by Ion Exchange

Lin, Johnson; Ruan, Shengzhe; Chen, Peilin

doi:10.1080/00986449808912707

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…Because immobilization of whole cells provides the way for the entrapment of multi-step and cooperative enzyme system present in the intact cells, repetitive use, and improved stability, it is feasible for long-term production [14][15][16][17][18][19][20][21]. Due to several advantages of immobilization, it has been applied in many bioreactors used in submerged filamentous fungal cultures including air-lift bioreactor, drum contactor, reciprocating jet bioreactor, tower fermentor, and hollow fiber bioreactor [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor

Chotisubha-anandha

Thitiprasert

Tolieng

et al. 2010

Bioprocess Biosyst Eng

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Rhizopus oryzae was immobilized on a cotton matrix in a static bed bioreactor. Compared with free cells in a stirred tank bioreactor, immobilized R. oryzae in this bioreactor gave higher lactic acid production but lower ethanol production. The highest lactic acid production rate (2.09 g/L h) with the final concentration of 37.83 g/L from 70 g/L glucose was achieved when operating the bioreactor at 700 rpm and 0.5 vvm air. To better understand the relationship between shear effects (agitation and aeration) and R. oryzae morphology and metabolism, oxygen transfer rate, fermentation kinetics, and lactate dehydrogenase activity were determined. In immobilized cell culture, higher oxygen transfer rate and lactic acid production were achieved but lower lactate dehydrogenase activity was found as compared with those in free cell culture operated at the same conditions. These results clearly imply that mass transport was the rate controlling step in lactic acid fermentation by R. oryzae.

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Section: Introductionmentioning

confidence: 99%

Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor

Chotisubha-anandha

Thitiprasert

Tolieng

et al. 2010

Bioprocess Biosyst Eng

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“…Recently, there have been increasing interest in fungal fermentation with Rhizopus oryzae to produce optically pure L()-lactic acid (Dong et al, 1996;Hang and Suntornsuk, 1994;Kosakai et al, 1997;Lin et al, 1998;Park et al, 1998;Soccol et al, 1994a,b;Sun et al, 1999;Suntornsuk and Hang, 1994;Woiciechowski et al, 1999;Yin et al, 1998;Zhou et al, 1999). The fungal fermentation can also overcome the aforementioned disadvantages associated with bacterial fermentation.…”

Section: Introductionmentioning

confidence: 99%

Production of L(+)‐lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor

Tay

Yang

2002

Biotech & Bioengineering

160

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A rotating fibrous-bed bioreactor (RFB) was developed for fermentation to produce L(+)-lactic acid from glucose and cornstarch by Rhizopus oryzae. Fungal mycelia were immobilized on cotton cloth in the RFB for a prolonged period to study the fermentation kinetics and process stability. The pH and dissolved oxygen concentration (DO) were found to have significant effects on lactic acid productivity and yield, with pH 6 and 90% DO being the optimal conditions. A high lactic acid yield of 90% (w/w) and productivity of 2.5 g/L.h (467 g/h.m(2)) was obtained from glucose in fed-batch fermentation. When cornstarch was used as the substrate, the lactic acid yield was close to 100% (w/w) and the productivity was 1.65 g/L.h (300 g/h.m(2)). The highest concentration of lactic acid achieved in these fed-batch fermentations was 127 g/L. The immobilized-cells fermentation in the RFB gave a virtually cell-free fermentation broth and provided many advantages over conventional fermentation processes, especially those with freely suspended fungal cells. Without immobilization with the cotton cloth, mycelia grew everywhere in the fermentor and caused serious problems in reactor control and operation and consequently the fermentation was poor in lactic acid production. Oxygen transfer in the RFB was also studied and the volumetric oxygen transfer coefficients under various aeration and agitation conditions were determined and then used to estimate the oxygen transfer rate and uptake rate during the fermentation. The results showed that the oxygen uptake rate increased with increasing DO, indicating that oxygen transfer was limited by the diffusion inside the mycelial layer.

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“…The filamentous fungal morphology often causes difficulties in agitation and aeration because of its complicated effects on the rheological property of the fermentation broth and reactor hydrodynamics. This morphological problem has been alleviated by controlling fungal growth into small pellets (13,16) or immobilization in porous materials by entrapment (18,19,27) or on solid surfaces by attachment (20). However, long-term performance of these fungal fermentation systems have not been well studied (27,28), and further improvements in lactic acid yield and production rate are needed in order to compete with the commonly used bacterial fermentation process.…”

Section: American Chemical Societymentioning

confidence: 99%

“…The highly branched fungal mycelia may cause complex (viscous) broth rheology and difficulty in mixing and aeration in the conventional agitated tank fermentor (14)(15)(16). Various cell immobilization methods to control the cell morphology and to achieve high cell density and high reaction rate have been studied (18)(19)(20)(21)(22)(23). In general, higher lactic acid yield and productivity were achieved with immobilized cells than those from free mycelial cells, partially due to reduced cell growth and increased specific cell productivity.…”

Section: Introductionmentioning

confidence: 99%

Controlling Filamentous Fungal Morphology by Immobilization on a Rotating Fibrous Matrix to Enhance Oxygen Transfer and L(+)-Lactic Acid Production by Rhizopus oryzae

Thongchul

Yang

2003

ACS Symposium Series

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Filamentous fungi are widely used in industrial fermentations. However, the filamentous morphology is usually difficult to control and often cause problems in conventional submerged fermentations. The fungal morphology has profound effects on mass transfer, cell growth, and metabolite production. Controlling the filamentous morphology by immobilization on a rotating fibrous matrix was studied for its effects on oxygen transfer and lactic acid production in aerobic fermentation by Rhizopus oryzae. Compared to the conventional stirred tank fermentor, the fermentation carried out in the rotating fibrous bed bioreactor (RFBB) resulted in a good control of the filamentous morphology, and improved oxygen transfer and lactic acid production from glucose. A high lactic acid concentration of 137 g/L with a high yield of 0.83 g/g and reactor productivity of 2.1 g/L·h was obtained with the RFBB in repeated batch fermentations. The process was stable and can be used for an extended operation period.

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Mathematical Model of L-Lactic Acid Fermentation in a RDC Coupled With Product Separation by Ion Exchange

Cited by 10 publications

References 15 publications

Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor

Improved oxygen transfer and increased l-lactic acid production by morphology control of Rhizopus oryzae in a static bed bioreactor

Production of L(+)‐lactic acid from glucose and starch by immobilized cells of Rhizopus oryzae in a rotating fibrous bed bioreactor

Controlling Filamentous Fungal Morphology by Immobilization on a Rotating Fibrous Matrix to Enhance Oxygen Transfer and L(+)-Lactic Acid Production by Rhizopus oryzae

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