Mathematical modeling of plus-strand RNA virus replication to identify broad-spectrum antiviral treatment strategies

Abstract

Plus-strand RNA viruses are the largest group of viruses. Many are human pathogens that inflict a socio-economic burden. Interestingly, plus-strand RNA viruses share remarkable similarities in their replication. A hallmark of plus-strand RNA viruses is the remodeling of intracellular membranes to establish replication organelles (so-called “replication factories”), which provide a protected environment for the replicase complex, consisting of the viral genome and proteins necessary for viral RNA synthesis. In the current study, we investigate pan-viral similarities and virus-specific differences in the life cycle of this highly relevant group of viruses. We first measured the kinetics of viral RNA, viral protein, and infectious virus particle production of hepatitis C virus (HCV), dengue virus (DENV), and coxsackievirus B3 (CVB3) in the immuno-compromised Huh7 cell line and thus without perturbations by an intrinsic immune response. Based on these measurements, we developed a detailed mathematical model of the replication of HCV, DENV, and CVB3 and show that only small virus-specific changes in the model were necessary to describe the in vitro dynamics of the different viruses. Our model correctly predicted virus-specific mechanisms such as host cell translation shut off and different kinetics of replication organelles. Further, our model suggests that the ability to suppress or shut down host cell mRNA translation may be a key factor for in vitro replication efficiency which may determine acute self-limited or chronic infection. We further analyzed potential broad-spectrum antiviral treatment options in silico and found that targeting viral RNA translation, especially polyprotein cleavage, and viral RNA synthesis may be the most promising drug targets for all plus-strand RNA viruses. Moreover, we found that targeting only the formation of replicase complexes did not stop the viral replication in vitro early in infection, while inhibiting intracellular trafficking processes may even lead to amplified viral growth.

Mathematical model of replication-mutation dynamics in coronaviruses

“…Zitzmann et al have used a version of the same model to analyse the effects of exosomal and viral RNA secretion in HCV [117], as well as to study the within-cell dynamics of dengue virus replication, with account for host immune responses [116]. Very recently, Zitzmann et al [118] considered a generic model of intracellular viral replication and showed that with very minor virus-specific changes, the model could be fit quite well to the measurements of viral replication of HCV, DENV and Coxsackie virus B3 (CVB3). This model has provided important insights into shutting down mRNA translation in host cells being a vital factor in controlling the efficiency of viral replication.…”