Mathematical modeling of poly[(R)-3-hydroxyalkanoate] synthesis by Cupriavidus necator DSM 545 on substrates stemming from biodiesel production

Vrana, Ivna; Lopar, Markan; Koller, Martin; Muhr, Alexander; Salerno, Anna; Reiterer, Angelika; Malli, Karin; Angerer, Hannes; Strohmeier, Katharina; Schober, Sigurd; Mittelbach, Martin; Horvat, Predrag

doi:10.1016/j.biortech.2013.01.126

Cited by 56 publications

(17 citation statements)

References 30 publications

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“…Controlled inflow of valeric acid and maintenance of its appropriate concentration was found to be the most crucial factor for regulating HV content in the P(3HB-co-3HV) copolymer. 93 Interesting results have been achieved by application of model-based PHA process optimization. However, it is important to remember that the use of this approach depends on careful and deliberate selection of only those feeding profiles and/or strategies, which could be easily and successfully implemented on industrial scale.…”

Section: Process Optimization Using Design-of-experiments Methodologymentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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Section: Process Optimization Using Design-of-experiments Methodologymentioning

confidence: 99%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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“…-alcohol-rich waste: methanol [94,95], crude glycerol phase (CGP) [96][97][98][99][100][101], fusel alcohols [102];…”

Section: Raw Materials For Pha Productionmentioning

confidence: 99%

Biomediated production of structurally diverse poly(hydroxyalkanoates) from surplus streams of the animal processing industry

Koller

Braunegg

2015

Polimery

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For commercial success, enhanced poly(hydroxyalkanoate) (PHA) production must address both material performance and economic aspects. Conventional PHA production consumes expensive feedstocks dedicated to nutrition. Switching to carbon-rich (agro)industrial side-streams alleviates industrial disposal problems, preserves food resources, and can be economically superior. Processes developed in the recently performed EU-FP7 project ANIMPOL resort to lipid-rich surplus streams from slaughterhouses and the rendering industry; these materials undergo chemical transformation to crude glycerol phase (CGP) and biodiesel. The saturated biodiesel share (SFAE) counteracts its applicability as a biofuel but, in addition to CGP, can be converted biotechnologically to PHAs. Depending on the applied microbial production strain and the selected carbon source (SFAE or CGP), thermoplastic short chain length PHA (scl-PHA), as well as elastomeric to latex-like medium chain length PHA (mcl-PHA), can be produced from these inexpensive feed stocks. The article illustrates the biotechnological conversion of animal-based CGP and SFAE towards poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), respectively, by Cupriavidus necator strain DSM 545. SFAE conversion towards mcl-PHAs consisting of various saturated and unsaturated building blocks by two pseudomonades, Ps. citronellolis DSM 50332 and Ps. chlororaphis DSM 50083, are also shown. Together with the kinetics of the bioprocesses, the results from the characterization of isolated samples of these structurally diverse biopolyesters are compared; data demonstrate the high versatility of biopolymer properties making them applicable in various fields of the plastic market. In addition to the need for inexpensive carbon feed stocks, the article points to further hot spots of the PHA-production chain that must be considered in order to lower the overall PHA production costs, and to enhance product quality. The benefits arising from multistage continuous cultivation production setups , namely high-throughput production of PHA of predefined composition and constant quality, are especially discussed. Finally, contemporary approaches towards environmentally and ecologically sustainable PHA recovery from biomass are summarized.

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“…putida KT2442 [11], the recently described Zobellella denitrificans [16], Cupriavidus necator [17, 18], Novosphingobium capsulatum [19], and Bacillus sp. [20].…”

Section: Introductionmentioning

confidence: 99%

Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

Hermann-Krauss

Koller

Muhr

et al. 2013

Archaea

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163

107

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The archaeon Haloferax mediterranei was selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol. Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester composition and volumetric productivity, indicating the feasibility of applying CGP as feedstock. Analysis of molar mass distribution revealed a weight average molar mass M w of 150 kDa and polydispersity P i of 2.1 for pure glycerol and 253 kDa and 2.7 for CGP, respectively; melting temperatures ranged between 130 and 140°C in both setups. Supplying γ-butyrolactone as 4-hydroxybutyrate (4HB) precursor resulted in a poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyvalerate-co-4-hydroxybutyrate] (PHBHV4HB) terpolyester containing 3HV (0.12 mol/mol) and 4HB (0.05 mol/mol) in the poly[(R)-3-hydroxybutyrate] (PHB) matrix; in addition, this process runs without sterilization of the bioreactor. The terpolyester displayed reduced melting (melting endotherms at 122 and 137°C) and glass transition temperature (2.5°C), increased molar mass (391 kDa), and a polydispersity similar to the copolyesters.

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Mathematical modeling of poly[(R)-3-hydroxyalkanoate] synthesis by Cupriavidus necator DSM 545 on substrates stemming from biodiesel production

Cited by 56 publications

References 30 publications

Strategies for Large-scale Production of Polyhydroxyalkanoates

Strategies for Large-scale Production of Polyhydroxyalkanoates

Biomediated production of structurally diverse poly(hydroxyalkanoates) from surplus streams of the animal processing industry

Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products

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