Mathematical modeling of the integrated process of mercury bioremediation in the industrial bioreactor

Głuszcz, P.; Petera, J.; Ledakowicz, S.

doi:10.1007/s00449-010-0469-8

Cited by 25 publications

(28 citation statements)

References 5 publications

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“…Most studies on the reduction kinetics of heavy metals such as mercury [ 54 ], arsenate [ 55 ], and chromate [ 56 ] reported a Haldane-type inhibition by the substrate metal ions. However, molybdenum reduction to Mo-blue showed a clear strong inhibition of Mo-blue production rate at high concentration of molybdenum with a calculated critical concentration of molybdenum that completely inhibited Mo-blue production at 108.22 mM.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Molybdenum Reduction to Molybdenum Blue byBacillussp. Strain A.rzi

Othman

Bakar

Halmi

et al. 2013

BioMed Research International

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Molybdenum is very toxic to agricultural animals. Mo-reducing bacterium can be used to immobilize soluble molybdenum to insoluble forms, reducing its toxicity in the process. In this work the isolation of a novel molybdate-reducing Gram positive bacterium tentatively identified as Bacillus sp. strain A.rzi from a metal-contaminated soil is reported. The cellular reduction of molybdate to molybdenum blue occurred optimally at 4 mM phosphate, using 1% (w/v) glucose, 50 mM molybdate, between 28 and 30°C and at pH 7.3. The spectrum of the Mo-blue product showed a maximum peak at 865 nm and a shoulder at 700 nm. Inhibitors of bacterial electron transport system (ETS) such as rotenone, sodium azide, antimycin A, and potassium cyanide could not inhibit the molybdenum-reducing activity. At 0.1 mM, mercury, copper, cadmium, arsenic, lead, chromium, cobalt, and zinc showed strong inhibition on molybdate reduction by crude enzyme. The best model that fitted the experimental data well was Luong followed by Haldane and Monod. The calculated value for Luong's constants p max, K s, S m, and n was 5.88 μmole Mo-blue hr−1, 70.36 mM, 108.22 mM, and 0.74, respectively. The characteristics of this bacterium make it an ideal tool for bioremediation of molybdenum pollution.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Molybdenum Reduction to Molybdenum Blue byBacillussp. Strain A.rzi

Othman

Bakar

Halmi

et al. 2013

BioMed Research International

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“…Kong et al 47 use the modified Gompertz model to model the growth of bacterium in the presence of chromium during the latter's reduction. The Haldane model has been cited for modelling the kinetics of metal-reducing bacterium such as mercury, 53 arsenate 54 and chromate. 55 It is a modification to the Monod model, which in turn was inspired by the Michaelis-Menten enzyme kinetics model.…”

Section: Resultsmentioning

confidence: 99%

Bioremoval of toxic Molybdenum using dialysis tubing

Shukor¹,

Shukor

2016

Chem. Eng. Res. Bull.

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Abstract:The toxicity of molybdenum to ruminants and its general toxicity to spermatogenesis in animals are increasingly being reported. Its contamination of aquatic bodies has been reported, and this necessitates its removal. In this work, we utilize the dialysis tubing method coupled with the molybdenum-reducing activity of S. marcescens strain Dr.Y6 to remove molybdenum from solution. The enzymatic reduction of molybdenum into the colloidal molybdenum blue traps the reduced product in the dialysis tubing. The initial rate of increase of Mo-blue product was determined using the modified Gompertz model while the resultant inhibition kinetics profile was carried out using the Haldane model. The calculated maximal rate of Mo-blue production was 153 mole (Moblue.hr) -1 and the concentration of molybdate resulting in the half-maximal rate of reduction (K s ), and the inhibition constant (K i ) were 0.22 and 506 mM, respectively. The results indicate that the system using dialysis tubing coupled with the Mo-reducing bacterium is a good candidate for a method for molybdenum bioremoval from solution.

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“…The reduction kinetics of metal-reducing microbes, nevertheless, is less studied. Currently, almost all the scientific studies on the reduction kinetics of heavy metals, for instance mercury (Głuszcz et al 2011), arsenate (Soda et al 2006) and chromate (Sukumar 2010), reported a Haldane-type inhibition. An investigation on uranium reduction, however, shows Monod kinetics (Truex et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

Halmi

Abdullah

Johari

et al. 2016

Rend. Fis. Acc. Lincei

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In the present work, the kinetics of hexavalent molybdenum reduction by the Serratia sp. strain MIE2 were investigated using several kinetic models, such as Monod, Haldane, Teissier, Aiba, Yano, Han and Levenspiel and Luong. The statistical analysis showed that the best model was Teissier, which had the lowest RMSE and AICc values, the highest adjusted R 2 values, and an F test and with a bias factor and an accuracy factor nearest to unity (1.0). The calculated value for the Teissier constants, such as p max , K s and K i , was 0.506 lmol Mo-blue h -1 , 6.53 mM and 29.41 mM, respectively. The effect of heavy metals showed that hexavalent molybdenum reduction by the strain MIE2 was inhibited by silver, mercury and copper with a total inhibition of 96, 97, and 45 %, respectively, at a concentration of 1 ppm. Otherwise, the Mo-reducing enzyme was inhibited by mercury and zinc with an inhibition of 88 and 65 %, respectively. Most of the respiratory inhibitors did not inhibit the Mo-reducing enzyme activity, indicating that the respiratory system in this bacterium is not the site of the hexavalent molybdenum reduction. The results obtained from this study could be useful for estimating the relationship between molybdenum-blue production and the molybdate concentration, which may be important during the up scaling of the molybdenum bioremediation process.

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Mathematical modeling of the integrated process of mercury bioremediation in the industrial bioreactor

Cited by 25 publications

References 5 publications

Kinetics of Molybdenum Reduction to Molybdenum Blue byBacillussp. Strain A.rzi

Kinetics of Molybdenum Reduction to Molybdenum Blue byBacillussp. Strain A.rzi

Bioremoval of toxic Molybdenum using dialysis tubing

Modelling the kinetics of hexavalent molybdenum (Mo6+) reduction by the Serratia sp. strain MIE2 in batch culture

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