Mating behavior and contact pheromones of <i>Batocera horsfieldi</i> (Hope) (Coleoptera: Cerambycidae)

Luo, S.-L.; Zhuge, Piao‐Piao; Wang, Man‐Qun

doi:10.1111/j.1479-8298.2011.00442.x

Cited by 7 publications

(14 citation statements)

References 18 publications

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“…As regards mating behaviour of B. horsfieldi , males show some mating‐attempt behaviours, including licking the maxillary palps and labial palps repeatedly and abdominal bending. This licking has been suggested to be related to contact sex pheromones (Luo et al. 2011).…”

Section: Discussionmentioning

confidence: 87%

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Chemosensory protein genes of batocera horsfieldi (hope): identification and expression pattern

Zhang

Wang

2012

J Applied Entomology

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Chemoreception is an essential feature for selection of host plants by insects. Chemosensory proteins (CSPs) are a group of small proteins (approximately 13 kDa) that are expressed widely in various insect sensory appendages. Batocera horsfieldi (Hope) is a major pest of Popolus and utilizes a variety of semiochemicals for its mating and oviposition. However, no CSP gene has been identified in B. horsfieldi. Here, to obtain the DNA sequences of B. horsfieldi CSPs, we used both bioinformatics and experimental approaches to analyse the antennal expressed sequence tags (ESTs). Among 649 EST clones, three unique CSP sequences (BhorCSP1, 2 and 3) were identified and characterized from B. horsfieldi (Hope) antennal cDNA libraries based on their four conserved cysteine residues, which have a characteristic spacing pattern. A phylogenetic tree of BhorCSPs and other insect CSPs showed that BhorCSP1 and BhorCSP2 are more similar to CSPs of Tribolium castaneum than BhorCSP3. BhorCSP3 is closely related to CSPs of the Dipteran, Culex quinquefasciatus. Using reverse transcription polymerase chain reaction (RT‐PCR) and real‐time Q‐PCR strategies, we examined the expression patterns of these three putative CSP genes in different tissues, sexes and developmental stages. Our analysis shows that all three genes were expressed in all tissues examined, including the antennae, labial palps, maxillary palps, legs, abdomen and wings but not in the head. Real‐time Q‐PCR results revealed that these CSP genes were expressed throughout the life of the adults, and the transcription levels of these genes depended on the sex, age and mating status of adults.

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Section: Discussionmentioning

confidence: 87%

“…2010). Chemical cues (contact sex pheromones) also play an important role in the recognition of females by male B. horsfieldi (Luo et al. 2011).…”

Section: Introductionmentioning

confidence: 99%

Chemosensory protein genes of batocera horsfieldi (hope): identification and expression pattern

Zhang

Wang

2012

J Applied Entomology

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“…Male B. horsfieldi displays a dashing behaviour for short‐range mate location, and previous studies have suggested that pheromones might be involved in this behaviour for this beetle species (Luo et al. ). Similar sex‐biased expression patterns of OBPs were also found in other insect species, such as SexiOBP2‐4 and SexiOBP10 of Spodoptera exigua (Zhu et al.…”

Section: Discussionmentioning

confidence: 90%

“…High level of BhorOBP2 expression in antennae of males on day 5 post‐eclosion might reflect the role for BhorOBP2 in mate‐seeking behaviour. Mating behaviour has been observed among female and male B. horsfieldi 5 days after emergence (Luo ). High levels of OBP expression in the early developmental stages of unmated males might be involved in locating suitable mates.…”

Section: Discussionmentioning

confidence: 99%

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Expression profile and ligand‐binding characterization of odorant‐binding protein 2 from Batocera horsfieldi (Hope)

Pan

Chen

et al. 2014

J Applied Entomology

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Odorant‐binding proteins (OBPs) are important components in insect olfactory systems that transport semiochemicals through the aqueous sensillum lymph to surface of olfactory receptor neurons. In this study, we cloned the cDNA of odorant‐binding protein 2 (BhorOBP2) in Batocera horsfieldi (Hope) and measured the level of the BhorOBP2 mRNA in a variety of tissues using quantitative reverse transcription and real‐time polymerase chain reaction. We found that the BhorOBP2 mRNA was primarily expressed in the antennae, the level of BhorOBP2 mRNA was higher in males than in females, and correlated with age and mating status. The BhorOBP2 mRNA was also expressed in the labial palps at relatively high level, suggesting that it might have a role in gestation as well. To better understand the functions of the BhorOBP2 protein in odorant reception, recombinant BhorOBP2 was expressed in Escherichia coli, and the binding specificity of the protein for 18 volatile developmentally relevant chemicals and host‐plant‐related metabolites were assessed using N‐phenyl‐1‐naphthylamine as a fluorescent probe. Competitive fluorescence binding assays showed that BhorOBP2 bound a broad range of host‐plant‐related odorants, including (E)‐2‐hexenal, 2‐methyl‐butanal, salicylaldehyde, (1S)‐(‐)‐verbenone, (Z)‐3‐hexen‐1‐ol, (E)‐2‐hexen‐1‐ol, β‐pinene and ethyl acetate. Our results imply that BhorOBP2 might play an important role in the selectivity and specificity of odorant reception.

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Identification of odorant‐binding proteins and functional analysis of antenna‐specific BhorOBP28 in Batocera horsfieldi (Hope)

Yi,

Chen,

et al. 2024

Pest Management Science

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BACKGROUNDThe important wood‐boring pest Batocera horsfieldi has evolved a sensitive olfactory system to locate host plants. Odorant‐binding proteins (OBPs) are thought to play key roles in olfactory recognition. Therefore exploring the physiological function of OBPs could facilitate a better understanding of insect chemical communications.RESULTSIn this research, 36 BhorOBPs genes were identified via transcriptome sequencing of adults' antennae from B. horsfieldi, and most BhorOBPs were predominantly expressed in chemosensory body parts. Through fluorescence competitive binding and fluorescence quenching assays, the antenna‐specific BhorOBP28 was investigated and displayed strong binding affinities forming stable complexes with five volatiles, including (+)‐α‐Pinene, (+)‐Limonene, β‐Pinene, (−)‐Limonene, and (+)‐Longifolene, which could also elicit conformation changes when they were interacting with BhorOBP28. Batocera horsfieldi females exhibited a preference for (−)‐Limonene, and a repellent response to (+)‐Longifolene. Feeding dsOBP19 produced by a bacteria‐expressed system with a newly constructed vector could lead to the knockdown of BhorOBP28, and could further impair B. horsfieldi attraction to (−)‐Limonene and repellent activity of (+)‐Longifolene. The analysis of site‐directed mutagenesis revealed that Leu7, Leu72, and Phe121 play a vital role in selectively binding properties of BhorOBP28.CONCLUSIONBy modeling the molecular mechanism of olfactory recognition, these results demonstrate that BhorOBP28 is involved in the chemoreception of B. horsfieldi. The bacterial‐expressed dsRNA delivery system gains new insights into potential population management strategies. Through the olfactory process concluded that discovering novel behavioral regulation and environmentally friendly control options for B. horsfieldi in the future.This article is protected by copyright. All rights reserved.

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Mating behavior and contact pheromones of Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae)

Cited by 7 publications

References 18 publications

Chemosensory protein genes of batocera horsfieldi (hope): identification and expression pattern

Chemosensory protein genes of batocera horsfieldi (hope): identification and expression pattern

Expression profile and ligand‐binding characterization of odorant‐binding protein 2 from Batocera horsfieldi (Hope)

Identification of odorant‐binding proteins and functional analysis of antenna‐specific BhorOBP28 in Batocera horsfieldi (Hope)

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