Mating induces an immune response and developmental switch in the<i>Drosophila</i>oviduct

Kapelnikov, Anat; Zelinger, Einat; Gottlieb, Yuval; Rhrissorrakrai, Kahn; Gunsalus, Kristin C.; Heifetz, Yael

doi:10.1073/pnas.0710997105

Cited by 85 publications

(118 citation statements)

References 38 publications

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“…Additionally, recent reports by Kapelnikov et al (33,34) show that mating up-regulates genes and proteins with known roles in muscle and epithelial differentiation in the oviduct (33), and that structural changes occur in the oviduct postmating (34). Analogous changes may occur in the uterus postmating.…”

Section: Uterine Conformational Changes Are Important For Maximal Spermmentioning

confidence: 99%

Acp36DE is required for uterine conformational changes in mated Drosophila females

Avila

Wolfner

2009

Proc. Natl. Acad. Sci. U.S.A.

114

134

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In a multitude of animals with internal fertilization, including insects and mammals, sperm are stored within a female's reproductive tract after mating. Defects in the process of sperm storage drastically reduce reproductive success. In Drosophila males, ''Acp'' seminal proteins alter female postmating physiology and behavior, and are necessary for several aspects of sperm storage. For example, Acps cause a series of conformational changes in the mated female's reproductive tract that occur during and immediately after mating. These conformational changes have been hypothesized to aid both in the movement of sperm within the female and in the subsequent storage of those sperm. We used RNAi to systematically knock down several Acps involved in sperm storage to determine whether they played a role in the mating-induced uterine conformational changes. Mates of males lacking the glycoprotein Acp36DE, which is needed for the accumulation of sperm in the storage organs, fail to complete the full sequence of the conformational changes. Our results show that uterine conformational changes are important for proper accumulation of sperm in storage and identify a seminal protein that mediates these changes. Four Acps included in this study, previously shown to affect sperm release from storage (CG9997, CG1656, CG1652, and CG17575), are not necessary for uterine conformational changes to occur. Rather, consistent with their role in later steps of sperm storage, we show here that their presence can affect the outcome of sperm competition situations.reproduction ͉ seminal protein ͉ sperm competition ͉ sperm storage

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Section: Uterine Conformational Changes Are Important For Maximal Spermmentioning

confidence: 99%

Acp36DE is required for uterine conformational changes in mated Drosophila females

Avila

Wolfner

2009

Proc. Natl. Acad. Sci. U.S.A.

114

134

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“…to the foreign proteins contained in the male's seminal fluid [43][44][45]. Finally, part of the changes in the female reproductive tract is in response to manipulative proteins from the male transcriptome in the form of accessory gland proteins (ACPs) [41,46,47], which stimulate female oogenesis and ovulation, as well as impede female remating [47].…”

Section: Short-term Gene Expression Dynamics In Response To Courtshipmentioning

confidence: 99%

“…Part of this is due to the female's innate transcriptomic preparations for embryo provisioning [43]. In addition to this, the female reproductive system stages an immune response [15]; also prevalent in mammalian transcriptomes [16] can occur when splicing is regulated by sex determination pathway or by sexhormone receptors imprinting either the paternal or maternal allele is expressed in the offspring involved in sex determination pathway in some insects [17], some medical conditions [18]; may be common in mammals [19,20] but this remains contentious [21] presumably, methylation patterns in the parental gametes are maintained in the zygote; alternatively, imprinting requires some sort of sensing mechanism to identify parent of origin rstb.royalsocietypublishing.org Phil Trans R Soc B 368: 20120047…”

Section: Short-term Gene Expression Dynamics In Response To Courtshipmentioning

confidence: 99%

Polyandry and sex-specific gene expression

Mank

Wedell

Hosken

2013

Phil. Trans. R. Soc. B

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Polyandry is widespread in nature, and has important evolutionary consequences for the evolution of sexual dimorphism and sexual conflict. Although many of the phenotypic consequences of polyandry have been elucidated, our understanding of the impacts of polyandry and mating systems on the genome is in its infancy. Polyandry can intensify selection on sexual characters and generate more intense sexual conflict. This has consequences for sequence evolution, but also for sex-biased gene expression, which acts as a link between mating systems, sex-specific selection and the evolution of sexual dimorphism. We discuss this and the remarkable confluence of sexual-conflict theory and patterns of gene expression, while also making predictions about transcription patterns, mating systems and sexual conflict. Gene expression is a key link in the genotype–phenotype chain, and although in its early stages, understanding the sexual selection–transcription relationship will provide significant insights into this critical association.

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“…However, while different female genotypes associate with differences in sperm competition outcomes, little is known about the specific genes or gene variants that underlie the differences in the female side of sperm competition. Gene expression analyses in singly mated (Lawniczak and Begun 2004;McGraw et al 2004McGraw et al , 2008McGraw et al , 2009Kapelnikov et al 2008) and doubly mated (Innocenti and Morrow 2010) females found numerous genes regulated by mating. These mating-regulated genes may include some that are important for sperm competition.…”

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confidence: 99%

Large Neurological Component to Genetic Differences Underlying Biased Sperm Use in Drosophila

2013

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Sperm competition arises as a result of complex interactions among male and female factors. While the roles of some male factors are known, little is known of the molecules or mechanisms that underlie the female contribution to sperm competition. The genetic tools available for Drosophila allow us to identify, in an unbiased manner, candidate female genes that are critical for mediating sperm competition outcomes. We first screened for differences in female sperm storage and use patterns by characterizing the natural variation in sperm competition in a set of 39 lines from the sequenced Drosophila Genetic Reference Panel (DGRP) of wild-derived inbred lines. We found extensive female variation in sperm competition outcomes. To generate a list of candidate female genes for functional studies, we performed a genome-wide association mapping, utilizing the common single-nucleotide polymorphisms (SNPs) segregating in the DGRP lines. Surprisingly, SNPs within ion channel genes and other genes with roles in the nervous system were among the top associated SNPs. Knockdown studies of three candidate genes (para, Rab2, and Rim) in sensory neurons innervating the female reproductive tract indicate that some of these candidate female genes may affect sperm competition by modulating the neural input of these sensory neurons to the female reproductive tract. More extensive functional studies are needed to elucidate the exact role of all these candidate female genes in sperm competition. Nevertheless, the female nervous system appears to have a previously unappreciated role in sperm competition. Our results indicate that the study of female control of sperm competition should not be limited to female reproductive tract-specific genes, but should focus also on diverse biological pathways. I N many organisms, sperm competition is an important source of reproductive variation and is critical to the reproductive success of both males and females. Sperm competition occurs when a female mates with and stores sperm from multiple males. Multiple mating creates a situation in which the males' sperm "compete" for successful fertilizations. While the ultimate outcome of a sperm competition depends on a complex interaction between male and female factors, the sexes often have different interests. Males are essentially in competition with other males, and while natural selection drives males to become better competitors with each other, it may result in males damaging the female through toxicity of seminal proteins (Wigby and Chapman 2004;Mueller et al. 2007). On the other hand, females benefit from simply being able to produce the most and highest-fitness offspring, and this may entail a specific response to male-produced molecules that influence sperm competition. The sexual conflict that arises from these different evolutionary goals of males and females implies that the genetic response to selection on sperm competition may be totally distinct in males and females. It remains largely unknown what genes drive this sexual antagon...

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Mating induces an immune response and developmental switch in theDrosophilaoviduct

Cited by 85 publications

References 38 publications

Acp36DE is required for uterine conformational changes in mated Drosophila females

Acp36DE is required for uterine conformational changes in mated Drosophila females

Polyandry and sex-specific gene expression

Large Neurological Component to Genetic Differences Underlying Biased Sperm Use in Drosophila

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