Mating Stimulation Required for Mating-Induced Estrous Abbreviation in Female Rats: Effects of Repeated Testing

Bennett, Amy L.; Blasberg, Meg E.; Blaustein, Jeffrey D.

doi:10.1006/hbeh.2002.1809

Cited by 19 publications

(6 citation statements)

References 23 publications

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“…The LI for rats treated with estradiol +oil was considerably lower (1.22 0.28) than that of rats that received EB+P (2.11 ± 0.12, P < 0.01, two-tailed t test). Furthermore, rats treated with EB+oil vehicle displayed an LQ of only 24%, whereas those rats treated with EB+P had an LQ of 75% (P b 0.001, two-tailed t test), which is above the accepted threshold for sexual receptivity of N60-70% (Edwards and Pfeifle, 1983;Bennett et al, 2002). These results demonstrate that under our experimental conditions, these animals were not in estrogen heats, and confirm that proceptive behaviors under these conditions are dependent on progesterone.…”

Section: Exp 2: Pr-dependent Proceptive Behaviorsupporting

confidence: 62%

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats

Molenda-Figueira

Williams

Griffin

et al. 2006

Hormones and Behavior

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The ovarian hormones, estradiol (E) and progesterone (P) facilitate the expression of sexual behavior in female rats. E and P mediate many of these behavioral effects by binding to their respective intracellular receptors in specific brain regions. Nuclear receptor coactivators, including Steroid Receptor Coactivator-1 (SRC-1) and CREB Binding Protein (CBP), dramatically enhance ligand-dependent steroid receptor transcriptional activity in vitro. Previously, our lab has shown that SRC-1 and CBP modulate estrogen receptor (ER)-mediated induction of progestin receptor (PR) gene expression in the ventromedial nucleus of the hypothalamus (VMN) and hormone-dependent sexual receptivity in female rats. Female sexual behaviors can be activated by high doses of E alone in ovariectomized rats, and thus are believed to be ER-dependent. However, the full repertoire of female sexual behavior, in particular, proceptive behaviors such as hopping, darting and ear wiggling, are considered to be PR-dependent. In the present experiments, the function of SRC-1 and CBP in distinct ER- (Exp. 1) and PR- (Exp. 2) dependent aspects of female sexual behavior was investigated. In Exp. 1, infusion of antisense oligodeoxynucleotides to SRC-1 and CBP mRNA into the VMN decreased lordosis intensity in rats treated with E alone, suggesting that these coactivators modulate ER-mediated female sexual behavior. In Exp. 2, antisense to SRC-1 and CBP mRNA around the time of P administration reduced PR-dependent ear wiggling and hopping and darting. Taken together, these data suggest that SRC-1 and CBP modulate ER and PR action in brain and influence distinct aspects of hormone-dependent sexual behaviors. These findings support our previous studies and provide further evidence that SRC-1 and CBP function together to regulate ovarian hormone action in behaviorally-relevant brain regions.

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Section: Exp 2: Pr-dependent Proceptive Behaviorsupporting

confidence: 62%

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats

Molenda-Figueira

Williams

Griffin

et al. 2006

Hormones and Behavior

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“…Another report from this laboratory (Bennett et al, 2002) has bearing on the interpretation of these results. We assessed the effects of extensive mating stimulation, which included intromissive stimulation by the male, on heat termination.…”

Section: Discussionmentioning

confidence: 97%

Non-intromissive mating stimuli are sufficient to enhance sexual behaviors in ovariectomized female rats

Blaustein

Farrell

Ghavami

et al. 2009

Hormones and Behavior

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When ovariectomized/adrenalectomized female rats, injected with subthreshold doses of estradiol are given copulatory stimulation by a male rat at half hour intervals, the level of lordosis gradually increases over the course of a few hours. We tested the hypothesis that paracopulatory behaviors (behaviors that occur repetitively prior to and between mounts), also generally considered to be heavily dependent on progesterone, are enhanced by this stimulation as well. We have reported previously that the enhancement of copulatory behavior is dependent to a large extent on intromissive stimulation by the male. In the present study, mating stimulation induced high levels of paracopulatory behaviors, as well as lordosis. Surprisingly, though, and in contrast to previous findings, this increase was seen not only in rats receiving intromissive stimulation, but in those receiving non-intromissive stimulation as well. Furthermore, intromissive stimulation induced high levels of rejection behavior. In a subsequent experiment, experimenter-induced, mechanical stimulation increased only rejection behaviors, not copulatory behavior. The results collectively demonstrate that, under the conditions used in these experiments, non-intromissive stimulation is sufficient for inducing both copulatory and paracopulatory behaviors in estradiol-primed rats. However, under the conditions used in these studies, intromissive stimulation increases rejection behaviors.

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“…Mating related stimuli induced by copulatory attempts by a male rodent, or manually by the experimenter, activate PRs in the absence of P [98–101]. Since mating stimulation induces DA release[102–105]and immediate early gene response (Fos) in PR-containing neurons [106], it is possible that DA activates PRs via ligand-independent activation mechanism.…”

Section: Ligand-independent Action Of Progestin Receptorsmentioning

confidence: 99%

Neural Progestin Receptors and Female Sexual Behavior

Mani

Blaustein

2012

Neuroendocrinology

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The steroid hormone, progesterone (P), modulates neuroendocrine functions in the central nervous system resulting in integration of reproduction and reproductive behaviors in female mammals. Although it is widely recognized that P’s effects on female sex behavior are mediated by the classical neural progestin receptors (PRs) functioning as ‘ligand-dependent’ transcription factors to regulate genes and genomic networks, additional mechanisms of PR activation also contribute to the behavioral response. Cellular and molecular evidence indicates that PRs can be activated in a ligand-independent manner by neurotransmitters, growth factors, cyclic nucleotides, progestin metabolites and mating stimuli. The rapid responses of P may be mediated by a variety of PR types, including membrane-associated PRs or extranuclear PRs. Furthermore, these rapid nonclassical P actions involving cytoplasmic kinase signaling and/or extranuclear PRs also converge with classical PR-mediated transcription-dependent pathways to regulate reproductive behaviors. In this review, we summarize some of the history of the study of the role of PRs in reproductive behaviors and update the status of PR-mediated mechanisms involved in the facilitation of female sex behavior. We present an integrative model of PR activation via crosstalk and convergence of multiple signaling pathways.

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Mating Stimulation Required for Mating-Induced Estrous Abbreviation in Female Rats: Effects of Repeated Testing

Cited by 19 publications

References 23 publications

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats

Nuclear receptor coactivators function in estrogen receptor- and progestin receptor-dependent aspects of sexual behavior in female rats

Non-intromissive mating stimuli are sufficient to enhance sexual behaviors in ovariectomized female rats

Neural Progestin Receptors and Female Sexual Behavior

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