Mating Test and &lt;i&gt;In Vitro&lt;/i&gt; Production of Perithecia by the Coffee Wilt Pathogen , &lt;i&gt;Gibberella xylarioides&lt;/i&gt; (&lt;i&gt;Fusarium xylarioides&lt;/i&gt;)

Adugna, Girma; Hindorf, H.; Steiner, U.; Dehne, H.-W.

doi:10.4314/ejbs.v6i1.39041

Cited by 4 publications

(8 citation statements)

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“…Detailed morphological studies and pathogenicity tests were carried out in a Ph.D. thesis by Adugna [28], who compared isolates from Arabica and Robusta coffee. In seedling tests it was proved that isolates from Arabica sources could only infect C. arabica and isolates from Robusta sources only infected C. canephora [28][29][30][31]. Therefore it was suggested, that the coffee wilt population should be classified into two formae speciales [32]: G. xylarioides f. sp.…”

Section: The Pathogensmentioning

confidence: 99%

“…In the field, black to violet perithecia of the perfect stage are formed on or beneath the bark at the base of the stem. For the first time, Adugna et al [31] produced perithecia of the perfect stage in vitro, when mating different isolates. The role of ascospores for distribution of the disease and in the infection process is not yet verified and needs to be investigated more precisely.…”

Section: The Pathogensmentioning

confidence: 99%

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A review of three major fungal diseases of Coffea arabica L. in the rainforests of Ethiopia and progress in breeding for resistance in Kenya

Hindorf

Omondi

2011

Journal of Advanced Research

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In a review of their own research the authors summarize incidences and distributions of the most important fungal diseases in Ethiopia and progress in breeding for resistance. Ethiopia, as the centre of origin for Coffea arabica, hosts a large diversity of germplasm. The incidences of diseases are based on observations in the montane rainforests of the southeast (Harenna) and southwest (Bonga, Berhane-Kontir, Yayu) of Ethiopia.

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Section: The Pathogensmentioning

confidence: 99%

Section: The Pathogensmentioning

confidence: 99%

A review of three major fungal diseases of Coffea arabica L. in the rainforests of Ethiopia and progress in breeding for resistance in Kenya

Hindorf

Omondi

2011

Journal of Advanced Research

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“…It is important to emphasize that the F. xylarioides arabica strain is only found in Ethiopia. Its discovery on wild forest coffee in Ethiopia highlights a wider risk to cultivated coffee and a threat to natural populations of coffee (Adugna et al, 2005;Girma et al, 2009). The arabica strain is clearly a threat to other countries growing this coffee variety and needs careful monitoring (Girma et al, 2007).…”

Section: Arabica-specific Fungal Populationsmentioning

confidence: 99%

“…Molecular differences between the arabica and robusta populations were further described (Adugna et al, 2005;Buddie et al, 2015;Olal et al, 2018).…”

Section: Genetic Diversity Of F Xylarioidesmentioning

confidence: 99%

“…(2005) subsequently showed that the pre‐1970s strains (first wave) were sexually incompatible with isolates associated with the second wave in the Democratic Republic of Congo, Uganda and Tanzania (Figure 4c,d). Molecular differences between the arabica and robusta populations were further described (Adugna et al., 2005; Buddie et al., 2015; Olal et al., 2018).…”

Section: The Pathogen and Host Specificitymentioning

confidence: 99%

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Coffee wilt disease: The forgotten threat to coffee

Peck,

Boa

2023

Plant Pathology

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Coffee is attacked by several highly damaging pests and diseases, which include coffee wilt disease (CWD). Despite a devastating impact in recent years, CWD receives little attention and its importance is downplayed or simply ignored. Memories are short and knowledge of past outbreaks fragmentary. Nearly two decades after the last major outbreaks, CWD has quietly faded into the background. This review describes a series of outbreaks of CWD across Africa, from Uganda to Guinea, from the first discovery in the 1920s to a hugely damaging recurrence that began in the 1970s and lasted through to the 2000s. This second wave had devastating impacts on growers and communities in the Democratic Republic of Congo and in Uganda. This review examines the origins of the disease, how and why it spread, and attempts to manage the outbreaks. Recent work on new pathogen variants is also considered. This review aims to recount these events and to evaluate the strategic successes and failures at national, regional and international levels in tackling the second wave of CWD.

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Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa.

Olal¹,

Olango²,

Kiggundu³

et al. 2018

J Plant Pathol Microbiol

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The present study presents the first report on the application of DNA-based polymerase chain reaction (PCR) for the specific detection and diagnosis of F usarium xylarioides (anamorph: G ibberrela xylarioides). Fusarium xylarioides is the causative agent of Coffee wilt disease (Tracheomycosis), and the disease is the most important economic constraint in Robusta coffee production in Uganda. The pathogen has two races, one pathogenic to Robusta coffee and the other to Arabica coffee, and not vice versa. Its laboratory diagnosis has been mainly based on microscopy, which is slow, has poor discriminative power, requires high expertise, only applicable on host plants with symptoms, and has since failed to detect the pathogen from the soil. Translation Elongation factor-1α (TEF-1α) gene from a F. xylarioides isolated from infected Robusta coffee plant was amplified by Fusarium genus specific primer then the PCR product sequenced. The sequence data was then used to design the specific primer. The primer-BLAST product was found to match only F. xylarioides sequences comprising 75% of the race pathogenic to Robusta and 25% to Arabica coffee. In vitro test by PCR showed the primer to be specific to only F. xylarioides amplifying a 284bp product and was able to differentiate F. xylarioides from all closely related species of Fusarium and other plant pathogens tested. More so it was able to amplify DNA from all the F. xylarioides isolates from different regions of Uganda, and amplified DNA concentrations as minute as 0.78 ng/µL.

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Mating Test and <i>In Vitro</i> Production of Perithecia by the Coffee Wilt Pathogen , <i>Gibberella xylarioides</i> (<i>Fusarium xylarioides</i>)

Cited by 4 publications

References 0 publications

A review of three major fungal diseases of Coffea arabica L. in the rainforests of Ethiopia and progress in breeding for resistance in Kenya

A review of three major fungal diseases of Coffea arabica L. in the rainforests of Ethiopia and progress in breeding for resistance in Kenya

Coffee wilt disease: The forgotten threat to coffee

Using Translation Elongation Factor Gene to Specifically Detect and Diagnose Fusarium xylaroides, a Causative Agent of Coffee Wilt Disease in Ethiopia, East and Central Africa.

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