Mating type and mating strategies in <i>Neurospora</i>

Metzenberg, Robert L.; Glass, N. Louise

doi:10.1002/bies.950120202

Cited by 312 publications

(176 citation statements)

References 15 publications

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“…Neurospora crassa (Herskowitz, 1988;Davis, 2000;Metzenberg and Glass, 1990), Podospora anserina (Zickler et al, 1995;Coppin et al, 1997;Raju and Perkins, 1994), Schizosaccharomyces pombe (Herskowitz, 1988;Kelly et al, 1988) and Ustilago maydis (Kahmann et al, 1995;Martinez-Espinosa et al, 1993), so that mating between individuals can be controlled. Development of the genetic system of S. cerevisiae has largely depended on the inactivation of HO so that strains are functionally heterothallic, i.e.…”

Section: Discussionmentioning

confidence: 99%

A new yeast genetic resource for analysis and breeding

Timberlake¹,

Frizzell²,

Richards

et al. 2010

Yeast

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We made a library of Saccharomyces cerevisiae F 1 hybrids from all possible crosses of 16 wild-type strains, including two common laboratory strains and two commercial winemaking varieties. Fourteen of the starting strains have been sequenced. Thus, the sequences of both genomes are known in 182 novel hybrids, and the sequence of one genome is known in 56. All tested strains sporulated. Fertilities were in the range 0-100%. Hybrids showed no more variation than parental strains for ethanol production, ethanol tolerance or growth at temperature extremes, but some F 1 s appeared to display hybrid vigour (heterosis). We tested four tetrads from one hybrid for their ability to grow at low temperature or in the presence of an inhibitory concentration of ethanol. Only one F 2 was as tolerant as the most tolerant F 0 parent. A few showed intermediate tolerance, but most were less tolerant than either parent or the F 1 hybrid, consistent with uncoupling of genes contributing to an optimized quantitative trait. The diversity and structure of the library should make it useful for analysis of genetic interactions among diverse strains, quantitative inheritance and heterosis, and for breeding.

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Section: Discussionmentioning

confidence: 99%

A new yeast genetic resource for analysis and breeding

Timberlake¹,

Frizzell²,

Richards

et al. 2010

Yeast

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“…The MAT locus has different alleles (idiomorphs) (Turgeon and Yoder, 2000), containing mating type genes that encode transcription factors controlling mate recognition and sexual processes (Metzenberg and Glass, 1990). In heterothallic ascomycetes, the mating types of isolates are determined by the presence of corresponding MAT idiomorphs in the haploid genome.…”

Section: Introductionmentioning

confidence: 99%

Mating type markers reveal high levels of heterothallism in Leptographium sensu lato

et al. 2016

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Species of Leptographium sensu lato (Ophiostomatales, Ascomycetes) are sap-stain fungi vectored by bark beetles (Coleoptera, Scolytinae) and some species cause or are associated with tree diseases. Sexual states have been reported for more than 30 species in this group and these have been treated in the sexual genus Grosmannia. No sexual state is known for at least 59 additional species and these reside in the genus Leptographium. The discovery of sexual states for species of Leptographium relies mainly on the presence of fruiting bodies on host tissue at the time of isolation and/or intensive laboratory mating studies, which commonly have a low levels of success. In this study, markers were developed to diagnose mating-type and to study sexual compatibility of species in Leptographium sensu lato using these markers. To achieve this objective, available mating type sequences for species of Leptographium sensu lato and Ophiostoma were obtained, aligned and used to design primers to amplify MAT genes in Grosmannia and Leptographium species. Using these primers, it was possible to amplify portions of the mating type genes for 42 species and to determine thallism, in many species for the first time. Surprisingly, the results showed that heterothallic and putatively heterothallic species are abundant (39 out of 42 species) in Leptographium sensu lato, and only three species were confirmed to be homothallic. The mating-type markers developed in this study will be useful for future studies concerning mating type and sexual compatibility of species in this genus.

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“…Although they occupy the same position in the genome, different mating type alleles have highly dissimilar sequences and gene contents and they are consequently referred to as idiomorphs named MAT1-1 and MAT1-2 (Metzenberg and Glass 1990;Turgeon and Yoder 2000). In heterothallic filamentous 3 ascomycetes, individual isolates contain either the MAT1-1 or the MAT1-2 idiomorph and sexual reproduction occurs only when isolates containing different MAT idiomorphs interact.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

et al. 2013

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Highlights· The mating-type loci in Leptographium procerum and L. profanum were characterized.· The PCR-based mating-type markers were developed for these two species.· Leptographium procerum and L. profanum had a heterothallic mating system and thus these species are heterothallic. · Cryptic sexual reproduction was suggested for a population of L. procerum. AbstractLeptographium procerum and the closely related species Leptographium profanum, are ascomycetes associated with root-infesting beetles on pines and hardwood trees, respectively.Both species occur in North America where they are apparently native. Leptographium procerum has also been found in Europe, China New Zealand, and South Africa where it has most probably been introduced. As is true for many other Leptographium species, sexual states 2 have never been observed in L. procerum or L. profanum. The objectives of this study were to clone and characterize the mating type loci of these fungi, and to develop markers to determine the mating types of individual isolates. To achieve this, a partial sequence of MAT1-2-1 was amplified using degenerate primers targeting the high mobility group (HMG) sequence. A complete MAT1-2 idiomorph of L. profanum was subsequently obtained by screening a genomic library using the HMG sequence as a probe. Long range PCR was used to amplify the complete MAT1-1 idiomorph of L. profanum and both the MAT1-1 and MAT1-2 idiomorphs of L.procerum. Characterization of the MAT idiomorphs suggests that the MAT genes are fully functional and that individuals of both these species are self-sterile in nature with a heterothallic mating system. Mating type markers were developed and tested on a population of L. procerum isolates from the USA, the assumed center of origin for this species. The results suggest that cryptic sexual reproduction is occurring or has recently taken place within this population.

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Mating type and mating strategies in Neurospora

Cited by 312 publications

References 15 publications

A new yeast genetic resource for analysis and breeding

A new yeast genetic resource for analysis and breeding

Mating type markers reveal high levels of heterothallism in Leptographium sensu lato

Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

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