matK and rbcL Gene Sequence Data Indicate that Saxifraga (Saxifragaceae) is Polyphyletic

Soltis, Douglas E.; Kuzoff, Robert K.; Conti, Elena; Gornall, R. J.; Ferguson, Keith

doi:10.2307/2446171

Cited by 65 publications

(76 citation statements)

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“…The RFLP analysis revealed molecular differences for 16 of 105 restriction enzyme assays between S. foliolosa and the other two species. These results are similar to a study of Saxifraga based on the cpDNA matK sequences (Soltis et al 1996; plus sequences in Genbank), in which S. nivalis and S. tenuis would appear to be more closely related to each other than to S. foliolosa. Based on comparison of the Genbank sequences, the single plant of S. tenuis differed from S. nivalis in four nucleotide substitutions, and from S. foliolosa in 79 substitutions.…”

Section: Chloroplast Dna Analysissupporting

confidence: 86%

“…The latter species was selected to provide perspective on the chloroplast DNA (cpDNA) data, based on its close phylogenetic relationship to the S. nivalis complex (Soltis et al 1996). DNA was extracted following Doyle and Doyle's (1990) CTAB total DNA extraction procedure as modified by Gillespie et al (1997).…”

Section: Chloroplast Dna Analysis Plant Materials and Dna Extractionmentioning

confidence: 99%

“…Some of the morphological variation in the complex, particularly within S. nivalis, may reflect this variation in habitat (Blondeau and Cayouette 2002;Aiken et al 2000*). Such high intraspecific variation is apparently typical of Saxifrages, particularly in arctic and alpine environments, and several studies have demonstrated that such variation may not be of taxonomic significance (Soltis et al 1996;Brysting et al 1996).…”

Section: Morphological Variabilitymentioning

confidence: 99%

“…In 1905, Small divided the genus Saxifraga into 13 genera in North America (in Small and Rydberg 1905; reviewed in Soltis et al 1996). Small treated S. nivalis as Micranthes nivalis (L.) Small, S. nivalis var.…”

mentioning

confidence: 99%

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A Systematic Analysis of the Alpine Saxifrage Complex (Saxifragaceae) in the Canadian Arctic Islands Using Morphology and Chloroplast DNA Data

Healy

Gillespie

2004

Can Field Nat

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The Saxifraga nivalis complex displays significant ecological, morphological and cytological variation. Most European studies suggest that the S. nivalis complex comprises two distinct species: Saxifraga nivalis sensu stricto and Saxifraga tenuis. However, the presence of intermediate morphotypes, inconsistencies in chromosomal counts and variability in morphological keys and descriptions have led to different taxonomic interpretations of the complex in North America. This study investigated the systematics of Canadian Arctic Island members of this complex from 157 specimens using 23 morphological characters. Principal component analysis of the morphological data revealed two adjacent clusters, corresponding to the two taxa and consistent with a close morphological similarity and the presence of hybrids. A preliminary restriction site analysis of five non-coding regions of the chloroplast genome, trnH-trnK, trnT-trnF, trnF-trnV, trnV-rbcL and rbcL-ORF106, was conducted using 21 restriction endonucleases. This analysis indicated a length difference between the trnT-trnF region of S. nivalis and that of S. tenuis, but no difference in restriction sites for any of the assayed regions. These results confirm that in the Canadian Arctic, the S. nivalis complex consists of two closely related, largely sympatric species, with notable morphological variability, and possible hybrids.

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Section: Chloroplast Dna Analysissupporting

confidence: 86%

Section: Chloroplast Dna Analysis Plant Materials and Dna Extractionmentioning

confidence: 99%

Section: Morphological Variabilitymentioning

confidence: 99%

mentioning

confidence: 99%

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A Systematic Analysis of the Alpine Saxifrage Complex (Saxifragaceae) in the Canadian Arctic Islands Using Morphology and Chloroplast DNA Data

Healy

Gillespie

2004

Can Field Nat

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“…Some ecologists have started using the barcoding approach to identify specific unknown plant samples for practical purposes (Li et al, 2009;Van de Wiel et al, 2009). Ongoing developments of new primers and improvements in sequencing techniques have facilitated the data-emergence process of plant barcoding (Soltis et al, 1996;Plunkett et al, 1997;Van de Wiel et al, 2009;Burgess et al, 2011). Recently, plant diversity belowground was determined using rbcL gene sequences as a core plant DNA barcoding marker .…”

Section: Dna Barcoding Of Arid Wild Plantsmentioning

confidence: 99%

DNA barcoding of arid wild plants using rbcL gene sequences

Bafeel¹,

Arif²,

Bakir³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. DNA barcoding is currently gaining popularity due to its simplicity and high accuracy as compared to the complexity and subjective biases associated with morphology-based identification of taxa. The standard chloroplast DNA barcode for land plants recommended by the Consortium for the Barcode of Life (CBOL) plant working group needs to be evaluated for a wide range of plant species. We therefore tested the potential of the rbcL marker for the identification of wild plants belonging to diverse families of arid regions. Maximum likelihood tree analysis was performed to evaluate the discriminatory power of the rbcL gene. Our findings showed that using rbcL gene sequences enabled identification of the majority of the samples (92%) to genus level and only 17% to species level.

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Chloroplast phylogenomics and the taxonomy of Saxifraga section Ciliatae (Saxifragaceae)

Yuan

Zhang³

et al. 2023

Ecology and Evolution

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matK and rbcL Gene Sequence Data Indicate that Saxifraga (Saxifragaceae) is Polyphyletic

Cited by 65 publications

References 0 publications

A Systematic Analysis of the Alpine Saxifrage Complex (Saxifragaceae) in the Canadian Arctic Islands Using Morphology and Chloroplast DNA Data

A Systematic Analysis of the Alpine Saxifrage Complex (Saxifragaceae) in the Canadian Arctic Islands Using Morphology and Chloroplast DNA Data

DNA barcoding of arid wild plants using rbcL gene sequences

Chloroplast phylogenomics and the taxonomy of Saxifraga section Ciliatae (Saxifragaceae)

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