Matriderm® versus Integra®: A comparative experimental study

Schneider, J; Biedermann, Thomas; Widmer, Daniel; Montaño, Irene; Meuli, Martin; Ernst, Rolf; Schiestl, Clemens

doi:10.1016/j.burns.2008.07.018

Cited by 129 publications

(67 citation statements)

References 16 publications

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“…Acellular dermal templates such as IntegraDRT ® , used in combination with split thickness skin transplants, have brought about certain improvements regarding long-term functional and cosmetic results, but this treatment requires a two-step, staged procedure (6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes

Braziulis

Diezi

Biedermann

et al. 2012

Tissue Engineering Part C: Methods

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Tissue engineering of clinically applicable dermo-epidermal skin substitutes is crucially dependent on the three-dimensional extracellular matrix, supporting the biological function of epidermal and dermal cells. This matrix essentially determines the mechanical stability of these substitutes to allow for safe and convenient surgical handling. Collagen type I hydrogels yield excellent biological functionality but their mechanical weakness and their tendency to contract and degrade does not allow producing clinically applicable transplants of larger sizes. We show here that plastically compressed collagen type I hydrogels can be produced in clinically relevant sizes (7 x 7 cm), and can be safely and conveniently handled by the surgeon. Most importantly, these dermo-epidermal skin substitutes mature into a near normal skin that can successfully reconstitute full thickness skin defects in an animal model. This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction. The final published version may differ from this proof. We show here that plastically compressed collagen type I hydrogels can be produced in clinically relevant sizes (7 x 7 cm), and can be safely and conveniently handled by the surgeon. Most importantly, these dermo-epidermal skin substitutes mature into a near normal skin that can successfully reconstitute full thickness skin defects in an animal model.

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Section: Introductionmentioning

confidence: 99%

Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes

Braziulis

Diezi

Biedermann

et al. 2012

Tissue Engineering Part C: Methods

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108

126

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“…Schneider demostró que a nivel experimental no existían diferencias en cuanto a la cantidad de dermis obtenida con estas matrices al comparar una lámina bicapa con otra lámina monocapa, en concreto, Integra® contra Matriderm®. Además, tampoco se objetivaron diferencias en la tasa de prendimiento de los injertos sobre los sustitutos dérmicos (12).…”

Section: Discussionunclassified

“…Sin embargo, se ha observado que la presencia de láminas de colágeno de 1mm de espesor no retrasa el prendimiento del injerto y no aumenta la tasa de reintervención para conseguir la cobertura (11). La aplicación de Matriderm® de 1 mm ha demostrado tener la misma tasa de prendimiento de los injertos, sin encontrar diferencias estadísticas en cuanto al espesor de dermis lograda cuando se compara con Integra® bicapa de 3 mm (12).…”

Section: Introductionunclassified

Terapia de vacío como adyuvante para el uso de sustitutos dérmicos monocapa

Martínez-Méndez

Bitrián

Rodriguez

et al. 2010

Cir. plást. iberolatinoam.

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ResumenEl método de elección para la cobertura de algunos defectos cutáneos es el autoinjerto de piel parcial; sin embargo, sufre retracciones que pueden adquirir gran relevancia sobre áreas especiales. Diferentes autores han intentado evitar estos déficits funcionales mediante la interposición de matrices de regeneración dérmica monocapa e injerto en un solo tiempo quirúrgico. Diseñamos un estudio para comparar la tasa de integración de los injertos sobre una matriz monocapa con y sin la aplicación de terapia de vacío tipo VAC® sobre ellos.Se aleatorizaron 20 pacientes con defectos cutáneos sobre áreas especiales en 2 grupos. Recogimos los datos epidemiológicos y la etiología del defecto (quemadura aguda, secuelas de quemadura o defectos secundarios a extracción de colgajo fasciocutáneo). En 10 casos (Grupo I) se aplicó matriz dérmica monocapa Matriderm ® más un injerto cutáneo de piel parcial, realizando un cambio de vendaje a los 7 días y luego cada 3-4 días hasta su estabilización. En otros 10 pacientes (Grupo II), tras realizar el mismo protocolo quirúrgico se aplicó VAC ® durante una semana y se cambió hasta la estabilización del injerto. La evaluación del prendimiento del injerto y su estabilidad la realizó un observador ciego. Se recogieron todas las complicaciones surgidas en el postoperatorio hasta 3 meses después de la estabilidad del injerto.Comprobamos la homogeneidad de ambos grupos para las variables epidemiológicas de los pacientes, así como para la etiología de los defectos. La media de tiempo hasta el prendimiento fue de 17,6 ± 8 días, de forma global con una tasa de complicaciones del 20%. Al estratificar en 2 grupos encontramos que las diferencias en la tasa de complicaciones no eran estadísticamente significativas. El tiempo hasta el prendimiento era de 21,4 ± 9 días en el grupo I, frente a 13,9 ± 4 días en el grupo II. Estas diferencias sí eran estadísticamente significativas.Como conclusión, la aplicación de matrices de regeneración dérmica monocapa es segura, con una tasa de prendimiento de los injertos sobre ella del 85%. El uso del VAC ® sobre las láminas monocapa de dermis sintética acorta el tiempo de integración de las mismas y el prendimiento de los injertos sobre ellas, con una tasa de complicaciones similar. AbstractSplit thickness autografts are the gold standard for wound coverage. However, scars and retractions are frequent after skin grafts, and would be severe over special regions. Different authors avoid these complications using a dermal substitute interposed between the wound and the skin graft in the first surgical time. A prospective study was designed to evaluate the split thickness skin graft uptake time over a monolayer matrix, with and without a vacuum assisted therapy device (VAC®).Twenty patients with a full thickness wound over a special region were randomized between 2 groups. Epidemiological data and wound etiology (acute burn, burn sequelae or donor site after a fasciocutaneous flap raised) were collected. Matriderm ® (mono layer dermal substitute) and a split ...

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“…Immunoincompetent female nu/nu rats, 8-10 weeks old (Harlan Laboratories, Venray, Netherlands), were prepared and anesthetized as described by Schneider et al 18 Skin analogs [containing palmar (n = 18) and nonpalmar (n = 9) fibroblast] were transplanted onto full-thickness skin defects created surgically on the backs of animals. Wound closure from surrounding rat skin was prevented and the skin substitutes were protected by surgical steel rings (26 mm in diameter) sutured to the skin defects using nonabsorbable polyester sutures (Ethibond Ò ; Ethicon, Zug, Zurich).…”

Section: Transplantation Of Dessmentioning

confidence: 99%

The Influence of Stromal Cells on the Pigmentation of Tissue-Engineered Dermo-Epidermal Skin Grafts

Biedermann

Böttcher‐Haberzeth

Klar

et al. 2015

Tissue Engineering Part A

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It has been shown in vitro that melanocyte proliferation and function in palmoplantar skin is regulated by mesenchymal factors derived from fibroblasts. In this study, we investigated in vivo the influence of mesenchymal-epithelial interactions in human tissue-engineered skin substitutes reconstructed from palmarand nonpalmoplantar-derived fibroblasts. Tissue-engineered dermo-epidermal analogs based on collagen type I hydrogels were populated with either human palmar or nonpalmoplantar fibroblasts and seeded with human nonpalmoplantar-derived melanocytes and keratinocytes. These skin substitutes were transplanted onto fullthickness skin wounds of immunoincompetent rats. Four weeks after transplantation the development of skin color was measured and grafts were excised and analyzed with regard to epidermal characteristics, in particular melanocyte number and function. Skin substitutes containing palmar-derived fibroblasts in comparison to nonpalmoplantar-derived fibroblasts showed (a) a significantly lighter pigmentation; (b) a reduced amount of epidermal melanin granules; and (c) a distinct melanosome expression. However, the number of melanocytes in the basal layer remained similar in both transplantation groups. These findings demonstrate that human palmar fibroblasts regulate the function of melanocytes in human pigmented dermo-epidermal skin substitutes after transplantation, whereas the number of melanocytes remains constant. This underscores the influence of sitespecific stromal cells and their importance when constructing skin substitutes for clinical application.

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Matriderm® versus Integra®: A comparative experimental study

Cited by 129 publications

References 16 publications

Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes

Modified Plastic Compression of Collagen Hydrogels Provides an Ideal Matrix for Clinically Applicable Skin Substitutes

Terapia de vacío como adyuvante para el uso de sustitutos dérmicos monocapa

The Influence of Stromal Cells on the Pigmentation of Tissue-Engineered Dermo-Epidermal Skin Grafts

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