Matrine inhibits invasiveness and metastasis of human malignant melanoma cell line A375 in vitro

Liu, Xiaoyan; Fang, Hong; Zhou, Yang; Wang, Xiaoyong; Ruan, Li-Ming; Fang, De-ren; Ding, Yongsheng; Wang, Yina; Zhang, Yu; Jiang, Xiaoling; Chen, Hongchao

doi:10.1111/j.1365-4632.2008.03627.x

Cited by 54 publications

(43 citation statements)

References 16 publications

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“…HPA-1 has been reported to degrade the extracellular matrix and facilitate the process of the invasion and metastasis of carcinoma (Liu et al, 2008;Yingying et al, 2009;Wagner et al, 2012). A predominant correlation was observed between HPA-1 expression and tumor invasion and lymphatic metastasis in gastric carcinoma (Endo et al, 2001;Takaoka et al, 2003), and this finding is consistent with the results of our study.…”

Section: Discussionsupporting

confidence: 83%

Increased Levels of β-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2

Yang

Zhang

et al. 2015

DNA and Cell Biology

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To determine the expression of b-catenin, lymphoid enhancer-binding protein-1 (LEF-1), and heparanase-1 (HPA-1) and to evaluate these proteins' potential prognostic values in malignant acral melanoma without mutations in BRAF exons 11 and 15 and NRAS exons 1 and 2, specimens from 90 patients with wild-type BRAF and NRAS were assessed and analyzed by immunohistochemistry and western blotting. The positive expression of b-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 was observed in 36 (72%), 31 (62%), and 32 (64%) of the detected acral melanomas, respectively. The expression of b-catenin, lymphoid enhancerbinding protein-1, and heparanase-1 was not correlated with gender, age, or diseased body parts ( p > 0.05), but was significantly positively correlated with the tumor node metastasis (TNM) stage and metastasis (correlation = 0.406 and 0.716, 0.397 and 0.582, 0.353 and 0.579; p = 0.040 and 0.0001, 0.0040 and 0.0001, 0.0120 and 0.0001, respectively). We also observed that the increased expression of b-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 was significantly correlated with decreased survival and poor prognosis ( p = 0.001, 0.010, and 0.023, respectively). A multifactorial analysis using Cox's regression model revealed that b-catenin, lymphoid enhancer-binding protein-1, heparanase-1, and the TNM stage were all independent factors in malignant melanoma (risk ratios were 7.294, 5.550, 5.622, and 4.794; p-values were 0.007, 0.018, 0.018, and 0.029, respectively). This study may provide the basis for the use of b-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 as novel targets in the treatment of malignant invasion and metastasis in acral melanoma cancer. The expression of b-catenin, LEF-1, and HPA-1 was assessed and compared in malignant melanoma with that of peritumoral tissue and benign nevus in the patients with negative mutations in BRAF exons 11 and 15 and NRAS exons 1 and 2. The study may provide the basis for b-catenin, LEF-1, and HPA-1 as new targets in the treatment of malignant invasion and metastasis in melanoma cancer.

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Section: Discussionsupporting

confidence: 83%

Increased Levels of β-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2

Yang

Zhang

et al. 2015

DNA and Cell Biology

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“…Kushen includes matrine, which is an alkaloid with the chemical formula of C 15 H 24 N 20 [5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Matrine has been shown to have a variety of effects in experimental studies, including a inhibitory effect on tumor cells [5,7,9,10,12,13,[16][17][18] and hepatitis [6,15] and a suppression of the proliferation of fibroblasts [8,11,14]. In ophthalmic experimental studies, matrine exhibited anti-inflammatory properties [19], was associated with a reduction in the recurrence rate of pterygia [20] and showed a preventive effect on proliferative vitreoretinopathy [21,22].…”

Section: Introductionmentioning

confidence: 99%

Effects of matrine on proliferation and apoptosis of cultured retinoblastoma cells

Zhao

Bai

et al. 2011

Graefes Arch Clin Exp Ophthalmol

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“…As a main alkaloid from Sophora flavescens Ait, matrine (C 15 H 24 N 2 O) also exhibits a variety of biological activities such as anti-inflammation, antivirus, anti-fibrosis, anti-arrhythmia and immunosuppression [7]. Moreover, recent studies have reported that matrine also possesses antitumor activities by inhibiting proliferation and inducing apoptosis against a wide range of cancers, including lung cancer, gastric cancer, breast cancer and so on [8,9,10,11,12,13,14]. Accordingly, our previous study demonstrated that matrine significantly inhibited growth of human nasopharyngeal carcinoma cells in a dose- and time-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

14-Thienyl Methylene Matrine (YYJ18), the Derivative from Matrine, Induces Apoptosis of Human Nasopharyngeal Carcinoma Cells by Targeting MAPK and PI3K/Akt Pathways in Vitro

Xie

Xiang

Wang

et al. 2014

Cell Physiol Biochem

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Background/Aims: Nasopharyngeal carcinoma (NPC) is a distinctive type of head and neck cancer with the highest incidence in South China. Previous studies have proved that matrine, a main alkaloid isolated from Sophora flavescens Ait, has antitumor activity against NPC. However, the effect is not so pronounced and the underlying mechanism remains largely unclear. Here we investigated whether 14-thienyl methylene matrine (YYJ18) that was derived from matrine could exert more effective suppression activity on NPC, along with the underlying mechanism. Methods: NPC cell lines CNE1, CNE2 and HONE1 were treated with YYJ18. Cell proliferation and apoptosis were determined by MTT assay and flow cytometry. Activation of mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathways were determined by Western blotting and quantitative RT-PCR. Results: YYJ18 remarkably inhibited proliferation and induced apoptosis of all three NPC cell lines in a dose-dependent manner, especially in CNE2 cells. Furthermore, YYJ18 treatment significantly suppressed phosphorylation of p38 in CNE2 cells, but upregulated phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and Akt. Next, alterations in downstream signaling were found, including activation of BCL2-associated X protein (Bax), caspase-3 and inactivation of B-cell CLL/lymphoma 2 (Bcl-2). Conclusion: We demonstrate the potent inhibitory effects of 14-thienyl methylene matrine on NPC cells for the first time, which could be mediated by modulation of MAPK and PI3K/Akt pathways.

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Matrine inhibits invasiveness and metastasis of human malignant melanoma cell line A375 in vitro

Abstract: These findings indicate that matrine inhibits the invasiveness and metastasis of A375 cells in vitro. The mechanisms may be linked to the inhibition of cellular proliferation, induction of apoptosis, and downregulation of heparanase mRNA and protein expression.

Cited by 54 publications

References 16 publications

Increased Levels of β-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2

Increased Levels of β-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2

Effects of matrine on proliferation and apoptosis of cultured retinoblastoma cells

14-Thienyl Methylene Matrine (YYJ18), the Derivative from Matrine, Induces Apoptosis of Human Nasopharyngeal Carcinoma Cells by Targeting MAPK and PI3K/Akt Pathways <b><i>in Vitro</i></b>

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