Matrix-assisted laser desorption/ionization mass spectrometric quantification of the mu opioid receptor agonist DAMGO in ovine plasma

Desiderio, Dominic M.; Wirth, Urs; Lovelace, Jerry L.; Fridland, Genevieve; Umstot, Edward S.; Nguyen, Thi M.‐D.; Schiller, Peter W.; Szeto, Hazel; Clapp, James F.

doi:10.1002/1096-9888(200006)35:6<725::aid-jms1>3.3.co;2-9

Cited by 12 publications

(16 citation statements)

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“…Sensitivity is an important parameter for the quantitative analysis of a peptide at the latter time‐points of pharmacokinetic studies of the maternal plasma and fetal plasma systems 1,11. Therefore, the detection limit of a solution of d 0 ‐[Dmt 1 ]DALDA was determined.…”

Section: Resultsmentioning

confidence: 99%

“…Salts and compounds with high molecular weights were removed. Protein precipitation11 was followed by SPE 10,14,15. d 5 ‐[Dmt 1 ]DALDA (10 pmol, 6.4 ng; 10 μL of a 1 pmol/μl stock solution) was added to plasma sample (200 μL) and the mixture was mixed thoroughly.…”

Section: Methodsmentioning

confidence: 99%

“…This new quantification method combines capillary HPLC, a nanospray ion source, and a quadrupole time‐of‐flight mass spectrometer. Compared with our previous methods for opioid‐receptor agonist quantification (LSIMS, MALDI‐TOF),9–11 this new quantification method has the advantages of on‐line detection and minimal processing steps; these advantages combine to save time and to minimize any sample loss. Molecular specificity and sensitivity are both improved with this system.…”

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confidence: 99%

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Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Wan

Desiderio

2003

Rapid Comm Mass Spectrometry

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The synthetic peptide [Dmt(1)]DALDA (Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine; 'super-DALDA') is a mu opioid-receptor agonist. On-line liquid chromatography/quadrupole time-of-flight mass spectrometry and the corresponding stable isotope-incorporated synthetic peptide internal standard were used to quantify [Dmt(1)]DALDA that had been extracted from ovine plasma samples. The [M+2H](2+) ion was used to construct the calibration curve, and the product ion was used for verification of the peptide. The detection sensitivity for the [Dmt(1)]DALDA [M+2H](2+) ion was 12.5 fmol and 50 fmol for the m/z 432.3 product ion. The concentration profile of [Dmt(1)]DALDA was determined from a set of ovine plasma samples. The molecular specificity of the peptide quantification was confirmed by tandem mass spectrometry (MS/MS).

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Wan

Desiderio

2003

Rapid Comm Mass Spectrometry

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“…A number of publications have described the use of MALDI‐MS for quantitative measurements,4–9 for example, for the measurement of enzyme activities 3,10,11. The inhomogeneous distribution of analytes within sample spots is the most serious problem in the use of MALDI‐MS for quantitative measurements.…”

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confidence: 99%

Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix‐assisted laser desorption/ionization mass spectrometry using ionic liquid matrices

Zabet-Moghaddam

Heinzle

Tholey

2003

Rapid Comm Mass Spectrometry

125

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A major problem hampering the use of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for quantitative measurements is the inhomogeneous distribution of analytes and matrices in solid sample preparations. The use of ionic liquids as matrices for the qualitative and quantitative analysis of low molecular weight compounds like amino acids, sugars and vitamins was investigated. The ionic liquid matrices are composed of equimolar combinations of classical MALDI matrices (sinapinic acid, alpha-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid) with organic bases. These matrix systems allow a homogenous sample preparation with a thin ionic liquid layer having negligible vapour pressure. This leads to a facilitated qualitative and quantitative measurement of the analytes compared with classical solid matrices.

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“…MALDI‐MS has the merits of sensitivity, tolerance to salts, applicability to complex mixtures, and throughput. Quantitative analysis by MALDI has been reported for proteins,17–19 peptides,17, 20 cyclosporin,21, 22 antibiotics,23 oligosaccharides,24 oligonucleotides,25 and the substrates and products of enzymatic reactions 26, 27. MALDI requires mixing and co‐crystallization with chemical matrices before measurements.…”

Section: Introductionmentioning

confidence: 99%

Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry

Okuno

Wada

2005

J. Mass Spectrom.

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The applicability of the matrix-free laser desorption/ionization on silicon mass spectrometry (DIOS-MS) to measuring serum drug levels was examined by analyzing serum salicylic acid. The optimized and simple solid-phase extraction (SPE) allowed good recovery, 88.9 +/- 5.8%, for 1.4 mM (200 mg/L) of salicylic acid in serum. The negative ion MS allowed measurements of deprotonated molecules without interference from other signals. Using a deuterium-labeled internal standard, good linearity was obtained in the 0.14 to 4.2 mM (20-600 mg/L) range, which was sufficient for monitoring the therapeutic anti-inflammatory dose. SPE followed by DIOS-MS is anticipated to be a method of measuring drug levels in blood and may allow high throughput analysis.

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Matrix-assisted laser desorption/ionization mass spectrometric quantification of the mu opioid receptor agonist DAMGO in ovine plasma

Cited by 12 publications

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Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix‐assisted laser desorption/ionization mass spectrometry using ionic liquid matrices

Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry

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Matrix-assisted laser desorption/ionization mass spectrometric quantification of the mu opioid receptor agonist DAMGO in ovine plasma

Cited by 12 publications

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Quantification of [Dmt1]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Quantification of [Dmt1]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix‐assisted laser desorption/ionization mass spectrometry using ionic liquid matrices

Measurement of serum salicylate levels by solid‐phase extraction and desorption/ionization on silicon mass spectrometry

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Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry

Quantification of [Dmt¹]DALDA in ovine plasma by on‐line liquid chromatography/quadrupole time‐of‐flight mass spectrometry