Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization

Faron, Matthew L.; Buchan, Blake W.; Ledeboer, Nathan A.

doi:10.1128/jcm.00868-17

Cited by 88 publications

(88 citation statements)

References 56 publications

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“…Although the underlying reason for this discrepancy is not known, it is reasonable to suggest that the bacterial cell wall components may play a role. In line with several previously published studies, the present study shows that all three separation methods have higher performance in identification of Gram-negative bacteria compared with Gram-positive bacteria [21][22][23]. Among the bacteria that could not be identified by the three methods analysed, the two groups, staphylococci and streptococci, dominated.…”

Section: Discussionsupporting

confidence: 90%

“…Almost all the previously published studies have showed that the identification of Gram-negative bacteria is more successful than Gram-positive bacteria by direct MALDI-TOF MS from blood cultures [21][22][23]. Although the underlying reason for this discrepancy is not known, it is reasonable to suggest that the bacterial cell wall components may play a role.…”

Section: Discussionmentioning

confidence: 99%

“…The present data were not used in guiding antimicrobial treatment. However, hitherto, published studies have showed that direct MALDI-TOF MS pathogen identification from positive blood culture have a positive impact on antimicrobial treatment in patients with BSIs [21,22]. It is reasonable to suggest that rapid BACpro® II with the present promising clinical results might be useful in rapid microbiological diagnostic of blood cultures in the clinical routine.…”

Section: Discussionmentioning

confidence: 99%

“…Hitherto, published studies showed variable results in the performance of different preparation methods ranging between 61 and 98% [21]. The analytical performance of the method, the experimental design, and the epidemiology of bacterial spp.…”

Section: Discussionmentioning

confidence: 99%

“…An alternative approach that can overcome these limitations is the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) directly from positive blood cultures [11]. Recently, it was shown that identification of microorganisms directly from positive blood cultures by MALDI-TOF MS significantly reduces time to identification of microorganisms compared with conventional methods [12].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

Kayın

Mert

Aydemir

et al. 2019

Eur J Clin Microbiol Infect Dis

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There are several approaches available for purifying microorganisms prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. In the present study, rapid BACpro® II (Nittobo Medical Co., Ltd., Tokyo, Japan), a new application, has been compared with Sepsityper® kit (Bruker Daltonics, Billerica, USA) and an in-house method. Samples were also tested with two modules, standard and Sepsityper®, identified in the Bruker MALDI-TOF MS. The bottles having monomicrobial growth were included in the study according to Gram staining results. In total, two hundred blood culture bottles were included but there was no growth in one of the subcultures so 199 blood culture bottles were studied prospectively. With the standard MALDI-TOF MS analysis, rapid BACpro® II could successfully identify microorganisms in 174/199 (87.4%) of the bottles where Sepsityper® kit and in-house method were successful in 136/199 (68.3%) and 114/199 (57.3%), respectively. When the MALDI-TOF MS data were analysed by Sepsityper® module, the identification rates were increased to 94.4%, 82.1% and 69.8% (p < 0.001), respectively. In the Sepsityper® module, 72/73 (98.6%) of Gram-negative and 97/106 (91.5%) of Grampositive microorganisms were detected by rapid BACpro® II method. The present study shows that rapid BACpro® II is a reliable preparation procedure and has higher rates of identification compared with Sepsityper® kit and in-house method. The use of the Sepsityper® module in blood cultures increases the chance of identification for all three methods studied.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

Kayın

Mert

Aydemir

et al. 2019

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

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MALDI‐TOF MS Analysis for Identification of Veterinary Pathogens from Companion Animals and Livestock Species

Timofte

Overesch

Spergser

2023

Microbiological Identification Using MALDI‐TOF and Tandem Mass Spectrometry

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Bloodstream infections in critically ill patients: an expert statement

et al. 2020

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Bloodstream infection (BSI) is defined by positive blood cultures in a patient with systemic signs of infection and may be either secondary to a documented source or primary-that is, without identified origin. Community-acquired BSIs in immunocompetent adults usually involve drug-susceptible bacteria, while healthcare-associated BSIs are frequently due to multidrug-resistant (MDR) strains. Early adequate antimicrobial therapy is a key to improve patient outcomes, especially in those with criteria for sepsis or septic shock, and should be based on guidelines and direct examination of available samples. Local epidemiology, suspected source, immune status, previous antimicrobial exposure, and documented colonization with MDR bacteria must be considered for the choice of first-line antimicrobials in healthcare-associated and hospital-acquired BSIs. Early genotypic or phenotypic tests are now available for bacterial identification and early detection of resistance mechanisms and may help, though their clinical impact warrants further investigations. Initial antimicrobial dosing should take into account the pharmacokinetic alterations commonly observed in ICU patients, with a loading dose in case of sepsis or septic shock. Initial antimicrobial combination attempting to increase the antimicrobial spectrum should be discussed when MDR bacteria are suspected and/or in the most severely ill patients. Source identification and control should be performed as soon as the hemodynamic status is stabilized. De-escalation from a broad-spectrum to a narrow-spectrum antimicrobial may reduce antibiotic selection pressure without negative impact on mortality. The duration of therapy is usually 5-8 days though longer durations may be discussed depending on the underlying illness and the source of infection. This narrative review covers the epidemiology, diagnostic workflow and therapeutic aspects of BSI in ICU patients and proposed up-todate expert statements.

show abstract

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization

Cited by 88 publications

References 56 publications

Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

Comparison of rapid BACpro® II, Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

MALDI‐TOF MS Analysis for Identification of Veterinary Pathogens from Companion Animals and Livestock Species

Bloodstream infections in critically ill patients: an expert statement

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