Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

Oviaño, Marina; Bou, Germán

doi:10.1128/cmr.00037-18

Cited by 113 publications

(90 citation statements)

References 154 publications

(166 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although MALDI-TOF MS was traditionally used for bacterial ID, recent novel applications of this approach suggest that it can be used to detect resistance markers such as β-lactamases by measuring bacterial growth in the presence of an antibiotic. 64,65 Although important advances have been made, MALDI-TOF MS recognizes only a limited number of resistance mechanisms and has a limited sensitivity, requiring more than 10 5 CFU/mL for accurate detection from clinical samples.…”

Section: Matrix-assisted Laser Desorption Ionization Time-of-flight Mmentioning

confidence: 99%

Novel Approaches to Hasten Detection of Pathogens and Antimicrobial Resistance in the Intensive Care Unit

Guillamet

Burnham

Kollef

2019

Semin Respir Crit Care Med

View full text Add to dashboard Cite

Antibiotic resistance is recognized as a key determinant of outcome in patients with serious infections influencing empiric antibiotic practices especially for critically ill patients. Within the intensive care unit (ICU), nosocomial infections and increasingly community-onset infections are caused by multidrug-resistant bacteria. Escalating rates of antibiotic resistance adds substantially to the morbidity, mortality, and cost related to infections treated in the ICU. Both gram-positive organisms, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci, and gram-negative bacteria, including Pseudomonas aeruginosa, Acinetobacter species, carbapenem-resistant Enterobacteriaceae, and extended spectrum β-lactamase producing organisms, are urgent threats. The rising rates of antimicrobial resistance have resulted in routine empiric administration of broad-spectrum antibiotics by clinicians to critically ill patients even when bacterial infection is microbiologically absent. Moreover, new broad-spectrum antibiotics are a challenge to use effectively while avoiding emergence of further resistance. Use of rapid diagnostic technologies (RDTs) will likely provide an important methodology for achieving this important balance. There is an urgent need for integrating the administration of new and existing antibiotics with RDTs in a way that is safe, cost-effective, applicable in all countries, and sustainable.

show abstract

Section: Matrix-assisted Laser Desorption Ionization Time-of-flight Mmentioning

confidence: 99%

Novel Approaches to Hasten Detection of Pathogens and Antimicrobial Resistance in the Intensive Care Unit

Guillamet

Burnham

Kollef

2019

Semin Respir Crit Care Med

View full text Add to dashboard Cite

show abstract

“…Furthermore, no reaction products were obtained when the experiment was performed with ceftriaxone or ceftazidime (not shown). Mass peaks of ~414 and ~370 Da have previously been reported as a consequence of cefotaxime degradation by ESBL-producing Enterobacteriaceae [11,13]. The ~370 Da peak corresponds to a decarboxylated and deacetylated derivative of cefotaxime in the hydrolyzed form, while the ~414 Da peak is deacetyl cefotaxime also hydrolyzed [14].…”

Section: Resultsmentioning

confidence: 86%

“…Normalized logRQ values below 0.2 represent negative results, while values above 0.4 indicate beta-lactamase activity. Normalized logRQ values between 0.2 and 0.4 correspond to an ambiguous result [12,13]. Next, the same protocol was applied to a collection of 17 clinical OXA-48-producing K. pneumoniae (8) and E. coli (9) isolates that did not coproduce extended-spectrum beta-lactamases (ESBL), AmpC beta-lactamases, or other carbapenemases, all of them susceptible to cefotaxime (MIC ≤ 1).…”

Section: Methodsmentioning

confidence: 99%

Analysis of the Degradation of Broad-Spectrum Cephalosporins by OXA-48-Producing Enterobacteriaceae Using MALDI-TOF MS

et al. 2019

Self Cite

View full text Add to dashboard Cite

The objective of the study was to evaluate the activity of OXA-48 against different broad-spectrum cephalosporins and to identify the reaction products by MALDI-TOF MS. The action of OXA-48 on cefotaxime, ceftazidime, and ceftriaxone was assessed by this method, using an Escherichia coli J53 transconjugant carrying only the ~62 Kb IncL plasmid containing the blaOXA-48 gene, and the same strain without any plasmid was included as a negative control. In addition, a collection of 17 clinical OXA-48-producing Enterobacteriaceae, which were susceptible to broad-spectrum cephalosporins, was evaluated. MALDI-TOF MS-based analysis of the E. coli transconjugant carrying the blaOXA-48-harboring plasmid, and also the clinical isolates, showed degradation of cefotaxime into two inactive compounds—decarboxylated and deacetylated cefotaxime (~370 Da) and deacetyl cefotaxime (~414 Da), both with the hydrolyzed beta-lactam ring. Reaction products were not obtained when the experiment was performed with ceftriaxone or ceftazidime. From a clinical point of view, our study supports the idea that the efficacy of cefotaxime against OXA-48-producing Enterobacteriaceae is doubtful, in contrast to ceftazidime and ceftriaxone which could be valid choices for treating infections caused by these bacteria. However, further clinical studies confirming this hypothesis are required.

show abstract

“…With the almost routine application of matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) in the clinical microbiology laboratory for species identification, wider exploration of additional generated data has been made regarding its suitability for the identification of antimicrobial resistance markers [8] and strain typing [9]. We therefore undertook a study to (i) evaluate the capability of MALDI-TOF MS as a rapid and accurate typing method compared with PFGE and MLST for CRKP isolates and (ii) describe the microbiologic characteristics (e.g.…”

Section: Introductionmentioning

confidence: 99%

Overestimated discriminatory power of MALDI-TOF mass spectrometry for typing of carbapenem-resistantKlebsiella pneumoniaeclones

et al. 2019

View full text Add to dashboard Cite

Homology surveillance of carbapenem-resistant Klebsiella pneumoniae (CRKP) is critical to monitor and prevent outbreaks of nosocomial infections. In the present study, a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF MS)-based method was evaluated as a rapid tool for typing CRKP in comparison with pulsed-field gel electrophoresis (PFGE) and multi locus sequence typing (MLST). Drug-resistant phenotypes and genotypes of 44 CRKP isolates were detected by microdilution broth method and polymerase chain reaction, and typed by PFGE, MLST and MALDI-TOF MS. Simpson's Index of Diversity was used to evaluate taxonomic diversity, Adjusted Rand Index (ARI) for congruence between the typing methods and Wallace coefficients (W) for the ability of either method to predict each other. Forty-four CRKP isolates of 15 sequence types (STs) produced either NDM-1 (n = 16), NDM-5 (n = 9) or KPC-2 (n = 19) carbapenemases. PFGE differentiated these isolates into 16 distinct types, and two deoxyribonucleic acid profiles were assigned to ST337 and ST11, respectively. MALDI-TOF MS failed to clearly delineate between clusters on dendrograms based on principal components analysis and main spectrum profile. The chosen parameters resulted in a maximum ARI of 0.310 (95% CI 0.088–0.531) between MALDI-TOF MS typing and the PFGE reference, indicating a low ability of the former to correctly identify related isolates. Likewise, the maximum W coefficient of 0.367 (95% CI 0.203–0.532) showed that MALDI-TOF MS had a lower predictive power than PFGE. We conclude that MALDI-TOF MS lacks the discriminatory power necessary for clone assignment of CRKP isolates and consequently cannot be considered as a rapid and creditable method for this purpose.

show abstract

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for the Rapid Detection of Antimicrobial Resistance Mechanisms and Beyond

Cited by 113 publications

References 154 publications

Novel Approaches to Hasten Detection of Pathogens and Antimicrobial Resistance in the Intensive Care Unit

Novel Approaches to Hasten Detection of Pathogens and Antimicrobial Resistance in the Intensive Care Unit

Analysis of the Degradation of Broad-Spectrum Cephalosporins by OXA-48-Producing Enterobacteriaceae Using MALDI-TOF MS

Overestimated discriminatory power of MALDI-TOF mass spectrometry for typing of carbapenem-resistantKlebsiella pneumoniaeclones

Contact Info

Product

Resources

About