2014
DOI: 10.1128/jcm.01025-14
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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Can Accurately Differentiate Aeromonas dhakensis from A. hydrophila, A. caviae, and A. veronii

Abstract: h Among 217 Aeromonas isolates identified by sequencing analysis of their rpoB genes, the accuracy rates of identification of A. dhakensis, A. hydrophila, A. veronii, and A. caviae were 96.7%, 90.0%, 96.7%, and 100.0%, respectively, by the cluster analysis of spectra generated by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

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Cited by 32 publications
(43 citation statements)
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“…Sedláçek et al (2012) identified 1 A. dhakensis among the 21 phenotypically identified A. hydro phila isolates, using the sequences of the cpn60 gene and also found that ribotyping with the EcoRI restriction enzyme was a suitable and efficient method for the identification of A. dhakensis. Other identification methods used in the recent literature for the identification of A. dhakensis include MALDI-TOF, which was able to correctly identify 29 of the 30 strains assayed (Chen et al 2014). The expected genus-characteristic resistance against ampicillin was observed for our A. dhakensis strain.…”
Section: A Rivuli Cect 7518 T (Fj969433)mentioning
confidence: 99%
“…Sedláçek et al (2012) identified 1 A. dhakensis among the 21 phenotypically identified A. hydro phila isolates, using the sequences of the cpn60 gene and also found that ribotyping with the EcoRI restriction enzyme was a suitable and efficient method for the identification of A. dhakensis. Other identification methods used in the recent literature for the identification of A. dhakensis include MALDI-TOF, which was able to correctly identify 29 of the 30 strains assayed (Chen et al 2014). The expected genus-characteristic resistance against ampicillin was observed for our A. dhakensis strain.…”
Section: A Rivuli Cect 7518 T (Fj969433)mentioning
confidence: 99%
“…Partial rpoB gene sequencing was performed as previously described (17). For 16S rRNA gene sequencing, two primers, 8FPL (5=-AGAGTTTGATCCTGGCTCAG-3=) and 1492RPL (5=-GGTTACCTTGTTACGACTT-3=) were used (21).…”
mentioning
confidence: 99%
“…Our previous study demonstrated that the Bruker Biotyper MALDI-TOF MS system, with implementation of a newly created database, can accurately identify other important human-enteropathogenic aeromonads, such as Aeromonas dhakensis, A. hydrophila, A. veronii, and A. caviae (17). However, few studies have investigated the ability of the Bruker Biotyper MALDI-TOF MS system to accurately identify pathogenic Vibrio species, particularly V. cholerae (10,(18)(19)(20).…”
mentioning
confidence: 99%
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