Matrix Effect Assessment of an Ion Chromatographic Method to Determine Inorganic Anions in Wastewater

Abstract

Inorganic anion monitoring is essential for bioreactor operation and is related for pollution control or energy and products recovery. However, there is a lack of studies validating methods for inorganic anions analyses in conditions compatible to those in bioreactor operations treating different types of wastewater. This paper provides a systematic statistical study and matrix-effect assessment for sugarcane vinasse, leachate, sewage and synthetic sewage. Sample preparation consisted of only a filtration and sample dilution. Cl − , NO 2 − , NO 3 − , PO 4 3− and SO 4 2− were determined in a Dionex ICS 5000® equipped with a chemical conductivity suppressor. Calibration curves were linear and well-adjusted between 2.5 and 50 mg L −1 for all the anions in all the tested matrices, except PO 4 3− and SO 4 2− in vinasse. A calibration range for PO 4 3− in all tested matrices was 5.0 to 100 mg L −1 , whereas a range from 5.0 mg L −1 to 50 mg L −1 was obtained for SO 4 2− in vinasse. All the anions yielded recoveries in the range of 85-115% for all the tested matrices. Relative standard deviations lower than 10 and 2% were achieved for peak areas and retention times, respectively. A signal enhancement was observed for all the tested matrices and all the anions. The matrix effect level varied from −1.7 (NO 2 − in vinasse) to −33.9% (Cl − in leachate). Sewage was the less affected matrix, while leachate gave higher matrix effects. Validation results and the matrix effect assessment showed that a simple sample preparation is suitable for multi-elemental analyses of inorganic anions for complex environmental samples.

Practical implications of methanotrophic denitrification as post‐treatment unit of anaerobic effluents in tropical areas

“…The column was maintained at 35 °C, and isocrat elution was used. The eluent was composed of a solution of sodium carbonate (4.5 mmol L −1 ) and sodium bicarbonate (0.5 mmol L −1 ) 33 …”

“…The eluent was composed of a solution of sodium carbonate (4.5 mmol L −1 ) and sodium bicarbonate (0.5 mmol L −1 ). 33 The total solids (TS) and total volatile solids (TVS) content of the inoculum and of the biomass attached to the polyurethane foams were determined according to the method 2540 from APHA. 34 By the end of experiment, the TS and TVS were analyzed.…”

Pilot-scale study of a structured bed reactor for nitrogen and organic matter removal from sanitary sewage: advances and design challenges

“…Alkalinity was monitored by following (DiLallo & Albertson, 1961)modified by (Ripley et al, 1986). NO2 --N and NO3 --N were determined according to (Costa et al, 2018). Two methods were applied to quantify NH4 + -N: the flow injection analysis (FIA) during Stages 1 and 2 and by Ion Chromatography in Stages 3 and 4.…”

Population Changes of Eubacteria, Sulfate-Reducing Bacteria and Methanogenic Archaea in an Anaerobic Reactor Processing Ethanol Distillery Vinasse

“…Isocratic elution (at a flow rate of1.0 mL. min −1 ) was used, and an analytical grade (> 99%) sodium carbonate (Aldrich) and sodium hydrogen carbonate (Sigma-Aldrich_Steinheim, Germany) were diluted in ultra-purified water to prepare the eluent at a concentration of 4.5 and 0.8 mM, respectively [13]. Sulfide concentration was determined by the colorimetric method on HACH DR-2800 ® spectrophotometers using a Sulfide Analysis Kit (Reagents Code 1816-32 and 1877-32).…”