Matrix Metalloproteinase-1 Produced by Human CXCL12-Stimulated Natural Killer Cells

Goda, Seiji; Inoue, Hiroshi; Umehara, Hisanori; Miyaji, Michihiko; Nagano, Yutaka; Harakawa, Nari; Imai, Hisao; Lee, Peter; MaCarthy, James B.; Ikeo, Takashi; Domae, Naochika; Shimizu, Yoji; Iida, Joji

doi:10.2353/ajpath.2006.050676

Cited by 43 publications

(44 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22 Studies also demonstrated that CXCL12 promotes natural killer cell migration into tissues. 23 A more recent study highlighted the importance of the CXCL12 axis for both neutrophil mobilization from the bone marrow and neutrophil recruitment to peripheral sites of infection in sepsis. 10 The results demonstrated that CXCL12 blockade prevents the release of neutrophils from the bone marrow during sepsis, resulting in a failure to increase blood and peritoneal neutrophil counts and a fivefold increase in peritoneal bacteria CFU during sepsis.…”

Section: Discussionmentioning

confidence: 99%

Combined treatment with a CXCL12 analogue and antibiotics improves survival and neutrophil recruitment and function in murine sepsis

et al. 2015

View full text Add to dashboard Cite

SummaryPrevious studies demonstrated that the CXCL12 peptide analogue CTCE-0214 (CTCE) has beneficial effects in experimental sepsis induced by caecal ligation and puncture (CLP). We examined the hypothesis that CTCE recruits neutrophils (polymorphonuclear leucocytes; PMN) to the site of infection, enhances PMN function and improves survival of mice in CLPinduced sepsis with antibiotic treatment. Mice with sepsis (n = 15) were administered imipenem (25 mg/kg) and CTCE (10 mg/kg) subcutaneously versus vehicle control at designated intervals post-CLP. CTCE treatment increased PMN recruitment in CLP-induced sepsis, as evidenced by increased PMN in blood, by 2Á4 AE 0Á6 fold at 18 hr, 2Á9 AE 0Á6 fold at 24 hr, and in peritoneal fluid by 2Á0 AE 0Á2 fold at 24 hr versus vehicle control. CTCE treatment reduced bacterial invasion in blood [colonyforming units (CFU) decreased 77 AE 11%], peritoneal fluid (CFU decreased 78 AE 9%) and lung (CFU decreased 79 AE 8% versus CLP vehicle). The improved PMN recruitment and bacterial clearance correlated with reduced mortality with CTCE treatment (20% versus 67% vehicle controls). In vitro studies support the notion that CTCE augments PMN function by enhancing phagocytic activity (1Á25 AE 0Á02 fold), increasing intracellular production of reactive oxygen species (32 AE 4%) and improving bacterial killing (CFU decreased 27 AE 3%). These composite findings support the hypothesis that specific CXCL12 analogues with ancillary antibiotic treatment are beneficial in experimental sepsis, in part, by augmenting PMN recruitment and function.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined treatment with a CXCL12 analogue and antibiotics improves survival and neutrophil recruitment and function in murine sepsis

et al. 2015

View full text Add to dashboard Cite

show abstract

“…CCL2 recruits inflammatory leukocytes (such as tumour-associated macrophages) in the tumour microenvironment and facilitates cancer cell progression [71]. CXC ligand (CXCL12) drives tumour invasion by induction of MMP-9 [72]. CXCR4 is responsible for metastatic implantation into other organs [73].…”

Section: Mechanism Of Actionsmentioning

confidence: 99%

Anticancer properties of nimbolide and pharmacokinetic considerations to accelerate its development

et al. 2016

View full text Add to dashboard Cite

Nimbolide is one of the main components in the leaf extract of Azadirachta indica (A. indica). Accumulating evidence from various in vitro and in vivo studies indicates that nimbolide possesses potent anticancer activity against several types of cancer and also shows potential chemopreventive activity in animal models. The main mechanisms of action of nimbolide include anti-proliferation, induction of apoptosis, inhibition of metastasis and angiogenesis, and modulation of carcinogen-metabolizing enzymes. Although multiple pharmacodynamic (PD) studies have been carried out, nimbolide is still at the infant stage in the drug development pipeline due to the lack of systematic pharmacokinetic (PK) studies and long-term toxicological studies. Preclinical PK and toxicological studies are vital in determining the dosage range to support the safety of nimbolide for first-in-human clinical trials. In this review, we will provide a comprehensive summary for the current status of nimbolide as an anticancer and chemopreventive lead compound, and highlight the importance of systematic preclinical PK and toxicological studies in accelerating the process of application of nimbolide as a therapeutic agent against various malignancies.

show abstract

“…Stimulation of rat NK cells by the prostaglandin PGE2 enhanced the secretion of MMP-1 and -3, and facilitated migration in the Matrigel invasion model (7). Human NK cell invasion into type I collagen has further been shown to be enhanced by the chemokine CXCL12 in a MMP-dependent manner (10). Stimulation of human NK cells by IL-18 has in addition been shown to increase their invasiveness in a Matrigel-based assay as well as their secretion of MMP-2, -9 and MT1-MMP (11).…”

Section: Introductionmentioning

confidence: 99%

Effects of IL-2 on MMP Expression in Freshly Isolated Human NK Cells and the IL-2-independent NK Cell Line YT

Edsparr

Speetjens

Mulder-Stapel

et al. 2010

Journal of Immunotherapy

View full text Add to dashboard Cite

Interleukin-2 is an important activation factor for NK cells but its effect on NK cell MMP production and matrix degradation is less well investigated. We have used freshly isolated human NK cells and the IL-2-independent NK cell line, YT, to investigate the effects of IL-2 stimulation on NK cell invasion of Matrigel as well as on MMP expression and production. In YT cells we found opposing early and late effects of IL-2 stimulation with an early (2h) increase in MMP-9 protein level and enhanced migration in the Matrigel invasion assay and by 30hrs a decreased mRNA expression of MMP-2, -9, -13, MT3- and MT6-MMP. We also found a pre-culture period of 48h with IL-2 to negatively affect YT cell migration. We furthermore found that freshly isolated human NK cells Matrigel invasion was MMP-dependent and it increased in response to IL-2. Importantly, in freshly isolated human NK cells we did not see a downregulation of MMPs after 24h IL-2 stimulation, but instead a significant upregulation of MT6-MMP mRNA. Because of the cellular localisation of MT6-MMP, which ensures a focalized proteolytic activity, and its high expression compared to the other MMPs in freshly isolated human NK cells makes it of interest to study further.

show abstract

Matrix Metalloproteinase-1 Produced by Human CXCL12-Stimulated Natural Killer Cells

Cited by 43 publications

References 46 publications

Combined treatment with a CXCL12 analogue and antibiotics improves survival and neutrophil recruitment and function in murine sepsis

Combined treatment with a CXCL12 analogue and antibiotics improves survival and neutrophil recruitment and function in murine sepsis

Anticancer properties of nimbolide and pharmacokinetic considerations to accelerate its development

Effects of IL-2 on MMP Expression in Freshly Isolated Human NK Cells and the IL-2-independent NK Cell Line YT

Contact Info

Product

Resources

About