Matrix Metalloproteinase‐3 and Vitamin D Receptor Genetic Polymorphisms, and Their Interactions with Occupational Exposure in Lumbar Disc Degeneration

Yuan, Hongling; Ying, Tang; Liang, Yupei; Ling, Lei; Xiao, Guo-Bing; Wang, Sheng; Zhang, Xia

doi:10.1539/joh.l8149

Cited by 72 publications

(57 citation statements)

References 25 publications

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“…In our study, we discerned no association between polymorphisms in MMP-1 and MMP-3 and the age of patients. A previous study investigating the association between disc degeneration caused by occupational trauma and polymorphisms in MMP-3 suggested that the gene-environment interaction could contribute to the development of lumbar disc degeneration in individuals expressing the 5A allele (Yuan et al, 2010). However, we found no association between the various genotypes of MMP-1 and MMP-3 and grades of degeneration in this study.…”

Section: Discussioncontrasting

confidence: 97%

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Eser¹,

Eser²,

Yüksel³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to identify the possible correlation between polymorphisms in matrix metalloproteinase (MMP)-1 and MMP-3 and their corresponding protein levels in disc 2 B. Eser et al. Genetics and Molecular Research 15 (3): gmr.15038669 tissues obtained from patients with lumbar disc herniation (LDH) using biochemical and immunohistochemical analyses. Blood and disc samples were obtained from 100 patients with LDH who underwent a lumbar microdiscectomy. Based on the radiological degeneration, the patients were diagnosed with grade 2, 3, or 4 LDH. MMP-1 -1607 1G/2G and MMP-3 -1171 5A/6A were analyzed by real-time polymerase chain reaction. The expressions of MMP-1 and MMP-3 were detected by biochemical and immunohistochemical analyses. We found no association between the MMP-1 polymorphism and disc degeneration and MMP-1 expression. However, patients expressing the 6A/6A and 5A/6A alleles of MMP-3 -11715A/6A showed higher MMP-3 expression, compared to those expressing the 5A/5A genotype. Additionally, the radiological degeneration grades were correlated with the histological degeneration scoring. Protein levels and immunopositive cell rates of MMP-1 and MMP-3 were associated with disc degeneration grades. Moreover, the MMP-1 and MMP-3 expression and the histological and radiological scores were positively correlated and the MMP-3 -11715A/6A polymorphism was associated with MMP-3 expression in herniated disc tissues. This study is the first to investigate polymorphisms in MMP-1 and MMP-3, as well as their corresponding protein expressions. We also quantified an association between the radiological degeneration grades and MMP-1 and MMP-3 expression. Further genomic studies on MMPs could focus on the utilization of MMP-1 and MMP-3 as markers for the prevention and treatment of this disease.

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Section: Discussioncontrasting

confidence: 97%

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Eser¹,

Eser²,

Yüksel³

et al. 2016

Genet. Mol. Res.

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“…Images were reported by a radiologist experienced in musculoskeletal MRI. We used four risk factors agreed by US-NIOSH (USA-National Institute for Occupational Safety and Health), to evaluate the occupational risk factors (Table 1) [12].…”

Section: Methods and Materials/patientsmentioning

confidence: 99%

Concomitance of Obesity and Overweight with Disc Height and Disc Herniation in Lumbar Spine

Arzpeyma

Mahfoozi

Pour

2016

IrJNS

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Background & Aim: Low back pain is the most debilitating condition, and can lead to decreased physical function, compromised quality of life, and psychological distress. Several studies have been conducted to recognize the risk factors of low back pain, but despite of strong relationship between obesity and low back pain, the underlying mechanisms are unclear. The aim of this study was to evaluate the association between obesity/overweight and disc height and disc bulging at each level and total levels in lumbar vertebra, considering occupational risk factors.Methods & Materials/Patients: This is a cross-sectional study on 102 patients with low back pain referring to MRI department in Poursina hospital in Rasht. Body mass index (kg/m 2 ) categories modified for Asian populations based on World Health Organization guidelines were used. Disc height and disc herniation were evaluated in sagittal T 1 , sagittal T 2 and axial T 2 MRI images. We used four risk factors agreed by US-NIOSH (USA-National Institute for Occupational Safety and Health), to evaluate the occupational risk factors. Regression test was used for statistical analysis. Results:Of the 102 patients, 36.3% were men and 63.7% were women. The age range was 15 to 75 years. Results showed that 26.5% of patients had normal body mass index, 39.2% were overweight and 34.3% were obese. Low, moderate, and high level of occupational risk factors were distributed as 36.3%, 17.6%, and 46.1%, respectively. Analysis with regression test showed that the relationship between obesity and overweight and disc height was negative at total levels and all levels, unless L 5 -S 1 . At L 5 -S 1 level, there was no association. There was a positive relationship between obesity and total score of disc herniation at L 1 -S 1 . There was no association between overweight and total score at this level. At L 1 -L 2 , L 2 -L 3 and L 5 -S 1 , there were no association between obesity and overweight and disc herniation, and at L 3 -L 4 and L 4 -L 5 , there were negative relationships between obesity and disc herniation. Conclusion:Our study noted the positive and significant association between obesity/overweight and disc degenerative changes in lumbar spine. Since there is abundant evidence in the literature demonstrating the strong association of disc degeneration on MRI with low back pain, prevention and treatment of being overweight or obese must be a public health priority. Keywords: Concomitance of Obesity and Overweight with Disc Height and Disc Herniation in Lumbar Spine

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“…in fact, excessive or inappropriate expression of MMPs may contribute to the pathogenesis of tissue destructive processes in a wide variety of diseases, including various types of cancer, invasion and metastasis of tumor cells, inflammation and degenerative diseases (9)(10)(11)(12)(13)(14). Many studies have investigated the association between polymorphic variants in MMP candidate genes and several diseases.…”

Section: Mmp-3 Polymorphism: Genetic Marker In Pathological Processesmentioning

confidence: 99%

MMP-3 polymorphism: Genetic marker in pathological processes (Review)

2010

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Abstract. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that are collectively capable of cleaving virtually all extracellular matrix (ecM) substrates and play an important role in diverse physiological and pathological processes. The activity of MMPs is controlled at multiple levels, and the transcriptional regulation of MMPs appears to represent a necessary step in its regulation. MMP-3 is a key member of the MMP family with broad substrate specificity, and is crucial to the connective tissue remodeling process. it is also involved in the turnover of the numerous ecM components. a common functional promoter polymorphism of MMP-3, 5a/6a, affects its activity and has been associated with various diseases. This polymorphism may be used as a genetic marker for certain pathologies to identify individual susceptibility. This review discusses various topics related to MMP-3 in pathological processes, with a focus on the 5a/6a polymorphism. Contents1. introduction 2. Matrix metalloproteinase-3 3. 5a/6a polymorphism of MMP-3 4. Pathological processes and the MMP-3 5a/6a polymorphism 5. Haplotype influence IntroductionThe evidence that individual characteristics play an important role in physiological and pathological processes has resulted in the targeted study of genetic polymorphisms in the clinical setting. in this context, mediators that degrade the extracellular matrix (ecM), such as matrix metalloproteinases (MMPs), are highlighted in studies involving inflammatory and degenerative diseases, and principally the prognosis and metastasis of cancer.degradation of the ecM is essential in many physiological processes, such as during development, growth and repair of tissue, and the microenvironment plays a central role in controlling both normal and transformed cell functions, as well as normal tissue integrity (1).MMPs are a pivotal family of zinc enzymes responsible for the degradation of ecM components, including basement membrane collagen, interstitial collagen, fibronectin and various proteoglycans, during normal remodeling and repair processes in development and inflammation. MMPs are also peptidase enzymes responsible for clotting factors, lipoproteins, latent growth factors and chemotactic and cell adhesion molecules (2,3). in this way, MMPs play a key role in the physiologic remodeling of tissues, including embryogenesis and tissue morphogenesis, angiogenesis, cell migration, proliferation, apoptosis, alteration of cell motility, effects on the immune system, wound repair and the inflammatory response (4).MMPs are a family of more than 25 enzymes. The expression of most MMPs is normally low in tissues and is induced when remodeling of the ecM is required. MMP gene expression is regulated primarily at the transcriptional level, but there is also evidence of the modulation of mrna stability in response to growth factors and cytokines (5). The promoter region of inducible MMP genes (i.e., MMP-3) shows remarkable conservation of regulatory elements, and their expression is induced ...

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Matrix Metalloproteinase‐3 and Vitamin D Receptor Genetic Polymorphisms, and Their Interactions with Occupational Exposure in Lumbar Disc Degeneration

Cited by 72 publications

References 25 publications

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Effects of MMP-1 and MMP-3 gene polymorphisms on gene expression and protein level in lumbar disc herniation

Concomitance of Obesity and Overweight with Disc Height and Disc Herniation in Lumbar Spine

MMP-3 polymorphism: Genetic marker in pathological processes (Review)

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