Matrix metalloproteinase-3 in articular cartilage is upregulated by joint immobilization and suppressed by passive joint motion

Leong, Daniel J.; Gu, Xiang; Li, Yonghui; Lee, Jonathan Y.; Laudier, Damien M.; Majeska, Robert J.; Schaffler, Mitchell B.; Cardoso, Luís; Sun, Hui

doi:10.1016/j.matbio.2010.02.004

Cited by 69 publications

(58 citation statements)

References 42 publications

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“…Previous studies display that reactive oxygen radicals induced by ionizing radiation may be responsible for the degradation of the physiologically important glycosaminoglycan hyaluronan [10,26,27]. Degradation of the proteoglycans and type II collagen present within the matrix of articular cartilage relates to mechanisms of various enzymes [2]. However, effects on these mechanisms are not sufficiently understood.…”

Section: Resultsmentioning

confidence: 99%

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Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Çiçek

2016

Acta Phys. Pol. A

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A load bearing tissue found at the ends of articulating bones, the articular cartilage provides low friction surfaces for efficient movement. Its mechanical properties are determined by the structure and composition of type II collagen, proteoglycans and interstitial fluid. This work investigates the effects of X-ray irradiation, previously shown to affect the biological properties of the articular tissue, on mechanical properties of articular cartilage using polarized light microscope for imaging and compressive modulus test applied to articular cartilage to determine the effects of the ionizing radiation on mechanical properties. According to the test results, the relaxation time is significantly longer in control than X-ray exposed samples, while the force is much higher showing that the X-ray irradiation causes the reduction in the stiffness of articular cartilage.

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Section: Resultsmentioning

confidence: 99%

“…Cartilage is a joint lubricating tissue composed primarily of proteoglycans and type II collagen [1], both determining the mechanical properties of articular cartilage and the latter being critically important for the tissue integrity [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of X-ray Irradiation on Articular Cartilage Mechanical Properties

Çiçek

2016

Acta Phys. Pol. A

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“…The expression of MMP-3 is primarily regulated at the level of transcription, where the promoter of the gene responds to various stimuli, including growth factors, cytokines, tumor promoters and oncogene products (20). MMP-3 expression is also induced in response to local conditions, such as mechanical loading (21) and inflammation (22).…”

Section: Matrix Metalloproteinase-3mentioning

confidence: 99%

MMP-3 polymorphism: Genetic marker in pathological processes (Review)

2010

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Abstract. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that are collectively capable of cleaving virtually all extracellular matrix (ecM) substrates and play an important role in diverse physiological and pathological processes. The activity of MMPs is controlled at multiple levels, and the transcriptional regulation of MMPs appears to represent a necessary step in its regulation. MMP-3 is a key member of the MMP family with broad substrate specificity, and is crucial to the connective tissue remodeling process. it is also involved in the turnover of the numerous ecM components. a common functional promoter polymorphism of MMP-3, 5a/6a, affects its activity and has been associated with various diseases. This polymorphism may be used as a genetic marker for certain pathologies to identify individual susceptibility. This review discusses various topics related to MMP-3 in pathological processes, with a focus on the 5a/6a polymorphism. Contents1. introduction 2. Matrix metalloproteinase-3 3. 5a/6a polymorphism of MMP-3 4. Pathological processes and the MMP-3 5a/6a polymorphism 5. Haplotype influence IntroductionThe evidence that individual characteristics play an important role in physiological and pathological processes has resulted in the targeted study of genetic polymorphisms in the clinical setting. in this context, mediators that degrade the extracellular matrix (ecM), such as matrix metalloproteinases (MMPs), are highlighted in studies involving inflammatory and degenerative diseases, and principally the prognosis and metastasis of cancer.degradation of the ecM is essential in many physiological processes, such as during development, growth and repair of tissue, and the microenvironment plays a central role in controlling both normal and transformed cell functions, as well as normal tissue integrity (1).MMPs are a pivotal family of zinc enzymes responsible for the degradation of ecM components, including basement membrane collagen, interstitial collagen, fibronectin and various proteoglycans, during normal remodeling and repair processes in development and inflammation. MMPs are also peptidase enzymes responsible for clotting factors, lipoproteins, latent growth factors and chemotactic and cell adhesion molecules (2,3). in this way, MMPs play a key role in the physiologic remodeling of tissues, including embryogenesis and tissue morphogenesis, angiogenesis, cell migration, proliferation, apoptosis, alteration of cell motility, effects on the immune system, wound repair and the inflammatory response (4).MMPs are a family of more than 25 enzymes. The expression of most MMPs is normally low in tissues and is induced when remodeling of the ecM is required. MMP gene expression is regulated primarily at the transcriptional level, but there is also evidence of the modulation of mrna stability in response to growth factors and cytokines (5). The promoter region of inducible MMP genes (i.e., MMP-3) shows remarkable conservation of regulatory elements, and their expression is induced ...

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“…Animal studies have demonstrated physiologic loading of joints exerts beneficial effects by suppressing the activity of proteolytic enzymes in healthy and arthritic rats. Passive joint motion prevented cartilage destruction due to inactivity and downregulated MMPs 1 and 3 (Leong et al 2010;Leong et al 2011). In antigen-induced rabbits, passive motion prevented proteoglycan loss and suppressed expression of MMP-1 (Ferretti et al 2005;Ferretti et al 2006).…”

Section: Anti-catabolic Effects Of Exercise On Cartilagementioning

confidence: 94%

“…Elevated levels of pro-inflammatory cytokines such as IL-1 and TNF-stimulate production of proteolytic enzymes matrix metalloproteinases (MMPs) and A Disintegrin and Metalloproteinase with Thrombospondin Motifs (ADAMTS) which mediate the cartilage destruction process in RA (Sun 2010). Studies have demonstrated physiological loading suppresses MMP and ADAMTS expression in both inflamed and noninflamed joints to exert protective effects on the synovium and articular cartilage (Ferretti et al 2005;Ferretti et al 2006;Leong et al 2010). The most recent progress on these mechanotransduction pathways which regulate the loading-induced anti-inflammatory and anti-catabolic responses will be presented, as well as possible crosstalk between these two pathways.…”

mentioning

confidence: 99%