Matrix metalloproteinase 7 and perlecan in oral epithelial dysplasia and carcinoma <i>in situ</i>: an aid for histopathologic recognition of their cell proliferation centers

Tilakaratne, W. M.; Kobayashi, Takanori; Ida‐Yonemochi, Hiroko; Swelam, Wael; Yamazaki, Manabu; Mikami, Toshihiko; Cg, Alvarado; Am, Shahidul; Maruyama, Satoshi; Cheng, Jun; Saku, Takashi

doi:10.1111/j.1600-0714.2009.00750.x

Cited by 24 publications

(23 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously reported that perlecan, a heparan sulfate proteoglycan of the basement membrane, as well as its lyase MMP-7 accumulated more frequently within the intercellular spaces of advanced grades of (true) epithelial dysplasia and CIS [10,11] . The extension of perlecan and MMP-7 immunopositivity seems to be similar to that of podoplanin, which may indicate that carcinoma cells are in active communication with the extracellular milieu within the zone circumscribed by the basement membrane.…”

Section: Discussionmentioning

confidence: 99%

“…However, the difficulties associated with making objective diagnoses of oral precancerous lesions based solely on HEstained sections are much better understood, and a number of studies have been performed to examine objective aids for such diagnoses that are applicable in daily clinical practice. For example, recent immunohistochemical studies have reported the significance of recognition of cellular differentiation (keratins) [6,7] , cell adhesion (Ecadherin and integrins) [8,9] , the intraepithelial extracellular matrix (perlecan) [10] , and their lyases (MMP-7) [11] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Expression of Podoplanin in Oral Carcinomas in situ and Squamous Cell Carcinomas

Funayama

Cheng²,

Maruyama

et al. 2011

Pathobiology

View full text Add to dashboard Cite

Objective: Podoplanin, a known lymphatic endothelial cell marker, has been reported to be expressed in various types of cancer. To elucidate the expression of podoplanin in precancerous lesions, we examined the immunohistochemical profiles of podoplanin in oral squamous epithelial lesions. Method: We studied a total of 298 foci of squamous cell carcinoma (SCC), carcinoma in situ (CIS), epithelial dysplasia, and hyperplastic and/or normal epithelial lesions by immunohistochemistry using D2-40. Results: There was no positivity for podoplanin in normal or hyperplastic epithelia, while all of the CIS and SCC foci stained positive. Approximately one third of the mild dysplasia foci (10 of 36 foci, 28%) and 80% of moderate dysplasia foci (78/98) showed grade 1 positive reactions (positive only in the 1st layer). Grade 2 reactions (up to 4th layer) were seen in 4 of 98 moderate dysplasia foci (4%), 29 of 74 CIS foci (39%), and 3 of 30 SCC foci (10%). Grade 3 reactions (to more than 5th layer) were found in 35 (47%) CIS foci and 26 (87%) SCC foci. Conclusions: The relationship between the present histological categorization and podoplanin grade was statistically significant. D2-40 expression is considered to be related to the severity of oral precancerous lesions.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Expression of Podoplanin in Oral Carcinomas in situ and Squamous Cell Carcinomas

Funayama

Cheng²,

Maruyama

et al. 2011

Pathobiology

View full text Add to dashboard Cite

show abstract

“…The lower half basaloid cells are Ki-67-positive, and they lack E-cadherin due to hypermethylation of its gene promoter region but show nuclear translocation of β-catenin [11]. In addition, an intercellular deposit of perlecan [12,13] with enhanced expression levels of MMP-7 [13], podoplanin [7], or Wnt target molecules [11] has been demonstrated in the lower half of two-phase dysplasia. Based on these lines of evidence, it is now possible to clearly separate CIS from epithelial dysplasia.…”

Section: Introductionmentioning

confidence: 95%

Intraepithelially entrapped blood vessels in oral carcinoma in-situ

et al. 2012

View full text Add to dashboard Cite

It can be difficult to make a certain diagnosis in case of an oral borderline malignant lesion on hematoxylin-eosin-stained sections only. Furthermore, assessment of surgical margins of borderline lesions is difficult with the naked eye. We set out to determine the topographical distribution of capillary blood vessels within the epithelial zone and to assess its use as an aid for histopathological diagnosis and a framework for clinical assessment of lesional margins using optical techniques, such as narrow-band imaging (NBI) endoscopy. Capillary blood vessels entrapped in the epithelial compartment, which we have designated as intraepithelially entrapped blood vessels (IEBVs), were examined for their frequency, location, and shape in normal mucosa, dysplasia, and carcinoma in-situ (CIS) of the tongue using immunohistochemistry for CD31 and type IV collagen. When counted per unit length of epithelial surface, IEBVs increased in number significantly in CIS (5.6 ± 2.8), which was two times more than in normal (1.9 ± 1.6) and dysplastic (2.4 ± 1.5) epithelia. In addition, IEBVs in CIS had compressed shapes with occasional obstruction or collapse with hemorrhage and were arranged perpendicular to and extending up to the epithelial surface. These characteristic IEBVs in CIS were considered to be generated by complex expansion of rete ridges due to carcinoma cell proliferation within the limited epithelial space determined by the basement membrane. The recognition of IEBVs was helpful in the differential diagnosis of oral CIS, and the present data provide a valuable frame of reference for detecting oral CIS areas using such NBI-based optical devices.

show abstract

“…We have also demonstrated the genetic mechanism for the loss of K13 as well as the reciprocal loss of K13 and emergence of K16/ K17 in oral CIS [5,6]. To consolidate our diagnostic criteria of oral CIS, we have introduced some other immunohistochemistry for podoplanin [7,8], perlecan [9][10][11][12], matrix metalloproteinase (MMP) 7 [10], tenascin [12], and β-catenin/E-cadherin [11] as diagnostic aids. More recently, we have also emphasized the formation of intraepithelial blood vessels as one of the histopathologic characteristics of oral CIS and as a driving force for dyskeratosis among cancer cells [13].…”

Section: Introductionmentioning

confidence: 96%

Protease-activated receptor 2 modulates proliferation and invasion of oral squamous cell carcinoma cells

et al. 2015

View full text Add to dashboard Cite

Matrix metalloproteinase 7 and perlecan in oral epithelial dysplasia and carcinoma in situ: an aid for histopathologic recognition of their cell proliferation centers

Cited by 24 publications

References 43 publications

Enhanced Expression of Podoplanin in Oral Carcinomas in situ and Squamous Cell Carcinomas

Enhanced Expression of Podoplanin in Oral Carcinomas in situ and Squamous Cell Carcinomas

Intraepithelially entrapped blood vessels in oral carcinoma in-situ

Protease-activated receptor 2 modulates proliferation and invasion of oral squamous cell carcinoma cells

Contact Info

Product

Resources

About