Matrix metalloproteinase 9 expression: new regulatory elements

Abstract

Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3′-untranslated regions (3′-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3′-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3′-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation.

A Single Nucleotide Polymorphism (rs1056629) in 3'-UTR of MMP-9 is Responsible for a Decreased Risk of Metastatic Osteosarcoma by Compromising its Interaction with microRNA-491-5p

“…Members of the MMP family take part in the degradation of the extracellular matrix in normal physiological processes, including tissue remodeling, reproduction and embryonic development, and they are involved in several human diseases [26,27]. Matrix metalloproteinase-9 [MMP9] could promote invasiveness and migration of cancer cells and works as an important oncogene [28].…”

The Association of Nailfold Capillaroscopy with Systemic Matrix Metalloproteinase-9 Concentration in Normal-Tension Glaucoma

“…Therefore, this enzyme is considered to be a pharmacological target for glaucoma treatment. 4,[31][32][33] However, no study has evaluated the relationship between the systemic MMP-9 concentration and the characteristics of glaucoma in NTG patients. In the present study, we focused on MMP-9 as a new target or a marker, and investigated the correlation of the systemic MMP-9 concentration with other parameters in NTG patients.…”

MiR-124 Promotes the Growth of Retinal Ganglion Cells Derived from Müller Cells

“…Role of miRNAs in the regulation of gene expression in RGCs was investigated previously [14]. In addition, miR-124 regulated the expression of NRP-1 in growth cone of RGCs, and promoted the binding of Sema3A with NRP-1 [14,15]. Therefore, this study aimed to explore the potential role of miR-124 in axon growth of ganglion cells derived from Müller cells both in vitro and in vivo.…”

“…miRNAs are small noncoding RNAs that recognize the 3′ untranslated region (UTR) of target genes and repress gene expression [12,13]. Role of miRNAs in the regulation of gene expression in RGCs was investigated previously [14]. In addition, miR-124 regulated the expression of NRP-1 in growth cone of RGCs, and promoted the binding of Sema3A with NRP-1 [14,15].…”