Matrix metalloproteinase activity is enhanced during corneal wound repair in high glucose condition

Abstract

MMP activity was enhanced in healing corneal epithelium, both in in vitro and in vivo diabetic models, suggesting its involvement in diabetic keratopathy.

Prolonged exposure to high glucose impaired cellular behavior of normal human corneal epithelial cells

“…The glucose concentration, we used in the present study, is extremely higher than that in vivo situation, and it seems to be a little higher than those in some other in vitro experiments. However, in the experiments of SV-40 transformed HCEC, McDermott et al 4 and Takahashi et al 5 used 38 mM and 31.2 mM glucose as a high glucose, respectively. Therefore, we selected 36 mM glucose as a high glucose in the present experiments.…”

“…Due to the increasing numbers of diabetic patients and intraocular surgery, the pathogenesis and treatment of diabetic keratopathy has attracted a lot of attention. [4][5][6][7][8] Corneal epithelial wound healing is considered to be a highly organized series of events in which the cells initially adhere to the substrate, migrate, proliferate, and cover the wounded area. 9,10 Disturbance at any phase of the above process could delay corneal epithelial wound healing in diabetic keratopathy.…”

Diabetic complications in the cornea

“…As diabetic corneas have slow and incomplete epithelial wound healing, and BM fragility and fragmentation, it is possible that proteinases are involved in these alterations. In fact, corneal epithelial cells in high glucose and rat diabetic corneal epithelium showed enhanced MMP activity during wound healing (Takahashi et al, 2000). Using gene microarrays, immunostaining, and zymography, our group has shown that ex vivo human diabetic corneas upregulated the expression of MMP-10 in the epithelium and keratocytes, MMP-3 in the keratocytes, and cathepsin F in the epithelium (Saghizadeh et al, 2001a; 2005).…”

Characterization of Transcript Levels for Matrix Molecules and Proteases in Ruptured Human Anterior Cruciate Ligaments

“…Expression of all considered ECM markers (collagen type I, collagen type III, and biglycan) is induced during ligament healing, with the exception of decorin that is reduced [18,40]. MMPs and TIMPs, critical indicators of tissue remodeling during healing, are upregulated in a wide range of healing tissue types [8,13,27,49]. MMPs-1, -2 and -9 along with TIMP-1 were of particular interest in this study due to their observed role in collagen breakdown in joint tissue [15,23,41].…”