2018
DOI: 10.3390/ijms19123923
|View full text |Cite
|
Sign up to set email alerts
|

Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B

Abstract: Matrix metalloproteinases (MMPs) are enzymes involved in periodontal tissue destruction. Hemagglutinin B (HagB) from the periodontal pathogen Porphyromonas gingivalis induces an elevated MMP response in dendritic cells, but responses from cultures of single-cell types do not reflect the local tissue environment. The objective of this study was to measure HagB-induced MMP responses in a transwell co-culture system containing dendritic cells, gingival epithelial (GE) keratinocytes, and CD4+ T-cells. Transwell co… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

0
22
0

Year Published

2019
2019
2023
2023

Publication Types

Select...
4
1

Relationship

1
4

Authors

Journals

citations
Cited by 18 publications
(22 citation statements)
references
References 28 publications
0
22
0
Order By: Relevance
“…Recombinant HagB was prepared by cloning hagB of P. gingivalis (1.4 kb) into the vector pQE31 (QIAGEN Inc., Valencia, CA USA) as described [1]. Escherichia coli M15(pREP4)pQE-31-TX1 was grown at 37 °C in selective Luria-base (LB) broth supplemented with ampicillin and kanamycin A.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
See 4 more Smart Citations
“…Recombinant HagB was prepared by cloning hagB of P. gingivalis (1.4 kb) into the vector pQE31 (QIAGEN Inc., Valencia, CA USA) as described [1]. Escherichia coli M15(pREP4)pQE-31-TX1 was grown at 37 °C in selective Luria-base (LB) broth supplemented with ampicillin and kanamycin A.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
“…E. coli was lysed in 6.0 M Guanidine-HCl, 0.1 M NaH 2 PO 4 , 0.01 M Tris Acid, pH 8.0. HagB was isolated from the E. coli lysate using a Profinity IMAC Ni-charged resin as described [1]. HagB was refolded using 6.0 M Urea, 0.5 M NaCl, 0.01 M Tris acid, 20.0% glycerol, pH 7.4 and then 3.0 M Urea, 0.5 M NaCl, 0.01 M Tris acid, 20.0% glycerol, pH 7.4.…”
Section: Experimental Design Materials and Methodsmentioning
confidence: 99%
See 3 more Smart Citations