Matrix metalloproteinases activity demonstrated in the infective stage of the nematodes, Angiostrongylus cantonensis

Lai, Shih‐Chan; Jiang, Shinn‐Jong; Chen, K. M.; Lee, H. H.

doi:10.1007/s00436-005-1484-6

Cited by 19 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From this perspective it is possible that the four metalloproteases found in A. americanum tick saliva could play important roles in facilitating tick feeding. It is also interesting to note that excretory-secretory proteomes of several blood feeding and/or dwelling parasites such as Haemonchus contortus [121] , Onchocerca volvulus [122], A. cantonensis [123, 124], Ancylostoma caninum [125], Ancylostoma ceylanicum [126], and Clonorchis sinensis [127] have metalloproteases, which regulate interactions of these parasites with their vertebrate hosts. It is also notable that metalloproteases are a major component of snake venom that is responsible for hemorrhage and may also interfere with the hemostatic system [128].…”

Section: Resultsmentioning

confidence: 99%

A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome

et al. 2014

View full text Add to dashboard Cite

BackgroundMultiple tick saliva proteins, the majority of which are unknown, confer tick resistance in repeatedly infested animals. The objective of this study was to identify the 24-48 h fed Amblyomma americanum tick saliva immuno-proteome. The 24-48 h tick-feeding phase is critical to tick parasitism as it precedes important events in tick biology, blood meal feeding and disease agent transmission. Fed male, 24 and 96 h fed female phage display cDNA expression libraries were biopanned using rabbit antibodies to 24 and 48 h fed A. americanum female tick saliva proteins. Biopanned immuno-cDNA libraries were subjected to next generation sequencing, de novo assembly, and bioinformatic analysis.ResultsMore than 800 transcripts that code for 24-48 h fed A. americanum immuno-proteins are described. Of the 895 immuno-proteins, 52% (464/895) were provisionally identified based on matches in GenBank. Of these, ~19% (86/464) show high level of identity to other tick hypothetical proteins, and the rest include putative proteases (serine, cysteine, leukotriene A-4 hydrolase, carboxypeptidases, and metalloproteases), protease inhibitors (serine and cysteine protease inhibitors, tick carboxypeptidase inhibitor), and transporters and/or ligand binding proteins (histamine binding/lipocalin, fatty acid binding, calreticulin, hemelipoprotein, IgG binding protein, ferritin, insulin-like growth factor binding proteins, and evasin). Others include enzymes (glutathione transferase, cytochrome oxidase, protein disulfide isomerase), ribosomal proteins, and those of miscellaneous functions (histamine release factor, selenoproteins, tetraspanin, defensin, heat shock proteins).ConclusionsData here demonstrate that A. americanum secretes a complex cocktail of immunogenic tick saliva proteins during the first 24-48 h of feeding. Of significance, previously validated immunogenic tick saliva proteins including AV422 protein, calreticulin, histamine release factor, histamine binding/lipocalins, selenoproteins, and paramyosin were identified in this screen, supporting the specificity of the approach in this study. While descriptive, this study opens opportunities for in-depth tick feeding physiology studies.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-518) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

A 24-48 h fed Amblyomma americanum tick saliva immuno-proteome

et al. 2014

View full text Add to dashboard Cite

show abstract

“…28 Metalloproteinase secreted in the infective larvae of A. cantonensis was associated with parasite dissemination or pathogenesis. 37 Conflicting results have been reported with regards to the relationship between CSF pleocytosis and MMP-9 levels. A relationship between increased MMP-9 levels in CSF and the number of leukocytes in various neurologic disorders was first reported by Gijbels and others.…”

Section: Discussionmentioning

confidence: 99%

Association of Matrix Metalloproteinase-9 and Tissue Inhibitors of Metalloproteinase-4 in Cerebrospinal Fluid with Blood-Brain Barrier Dysfunction in Patients with Eosinophilic Meningitis Caused by Angiostrongylus cantonensis

Tsai

Chung

Chen

et al. 2008

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

To evaluate possible blood-brain barrier (BBB) dysfunction caused by matrix metalloproteinase-9 (MMP-9) and its regulation by tissue inhibitors of metalloproteinase (TIMPs) in patients with eosinophilic meningitis caused by infection with Angiostrongylus cantonensis, 40 patients and 28 controls were included in this study. Concentrations of MMP-2, MMP-9, TIMP-1, and cerebrospinal fluid (CSF):serum albumin ratios (Q(Alb) values) were significantly increased in patients compared with controls. However, concentrations of TIMP-4 were significantly lower in patients. In contrast to MMP-2, proteolytic activity of MMP-9 detected by gelatin zymography was only observed in patients with eosinophilic meningitis. We detected higher levels of antibodies specific for A. cantonensis and higher Q(Alb) values and MMP-9 concentrations in CSF of patients with eosinophilic meningitis, Furthermore, the increase in the Q(Alb) value was significantly correlated with the increase in MMP-9 in patients. In parallel with CSF MMP-9, patients also showed an increase in CSF leukocyte counts. Gradual decreases in levels of Q(Alb), MMP-9, and TIMP-1 and increases in levels of TIMP-4 were observed in six patients during recovery from eosinophilic meningitis. These results suggest that the source of MMP-9 in CSF of patients with eosinophilic meningitis was probably associated with leukocytes migrating from peripheral blood to CSF. Activity of MMP-9 in CSF of patients could not be completely inhibited because of the decrease of TIMP-4, which may cause BBB dysfunction, as shown by higher Q(Alb) values in patients.

show abstract

“…The stronger gelatinolytic activity of L1 larvae may ensure its successful penetration through the mollusk's surface and muscular layers. However, L3 larvae may also make use of gelatinolytic enzymes to penetrate the vertebrate intestinal wall and reach the circulatory system, as already described for the infective stage of several parasite nematodes (Hotez et al 1990, Zhan et al 2002, Lai et al 2005, Lee & Yen 2005. L3 become adult worms inside the blood vessels, where these last will live their entire lives.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the secreted metalloproteases of Ancylostoma caninum appear to trigger the activation of the thirdstage infective larvae, including ecdysis and penetration of host tissues (Hotez et al 1990, Hawdon et al 1995, Williamson et al 2006. Metalloproteases have also been identified in extracts and excretory-secretory samples of A. cantonensis and may be associated with parasite dissemination and/or pathogenesis (Lai et al 2005). Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Metalloprotease activity has been already reported in several helminths (Lun et al 2003, Quiñones et al 2006, Williamson et al 2006, including Angiostrongylus cantonensis (Lee et al 2004, Lai et al 2005. These enzymes belong to a diverse group of enzymes that utilize coordination to a metal ion (usually zinc) to exert catalysis and have a powerful degrading effect on extracellular matrix components (Rawlings & Barrett 1995).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation