Matrix metalloproteinases in serum and the follicular fluid of women treated by in vitro fertilization

Horká, Petra; Malíčková, Karin; Jarosová, R; Janatková, I; Zima, Tomáš; Kalousová, Marta

doi:10.1007/s10815-012-9853-4

Cited by 19 publications

(20 citation statements)

References 24 publications

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“…In contrast, Lee et al found no correlation between fertilization rates and the levels of gelatinases in the follicular fluid. Although they demonstrated a significant difference between serum MMP-9 expression with implantation and pregnancy rates, as noted in previous studies, they found similar MMP-2 levels between pregnant and nonpregnant IVF-treated women [21,29,30]. They therefore suggested that MMP-9 expression in serum and in follicular fluid is essential for a successful pregnancy [30].…”

Section: Discussionmentioning

confidence: 53%

“…In animal studies, a correlation has been noted between decreased MMP concentrations in preovulatory follicular fluid and poor oocyte release and abnormal follicular involution [21]. In addition, the presence of gelatinolytic activity at the apex of the preovulatory follicle suggests that MMPs play a role in ovulation.…”

Section: Discussionmentioning

confidence: 98%

“…Baka et al [31] observed no correlation between the follicular fluid gelatinase levels and pregnancy rates. Higher serum MMP-2 levels have been reported in healthy pregnant women compared to IVF-treated women and healthy nonpregnant women, indicating that MMP-2 is involved in pregnancy [21,29,32]. MMP-2 levels might therefore serve as a predictor of a healthy pregnancy in women with normal ovulation, and MMP-9 could serve as a potential marker in IVF-treated pregnant women.…”

Section: Discussionmentioning

confidence: 98%

See 2 more Smart Citations

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Bilen

Tola

Oral

et al. 2014

Arch Gynecol Obstet

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 98%

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Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Bilen

Tola

Oral

et al. 2014

Arch Gynecol Obstet

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“…Production of MMPs (MMP9 and MMP2) by the control and heat-stressed cumulus-oocyte complexes Because higher MMP9 levels have been associated with the developmental competence of oocytes (Robert et al 2001), implantation and pregnancy rates (Lee et al 2005, Horka et al 2012, and fertility (Dubois et al 2000), their abundance in the conditioned maturation medium (generated as a part of the transcriptome profiling efforts depicted in Fig. 1) observed after 24 h IVM in the control and heatstressed oocytes was examined.…”

Section: Heat Stress Effects On Cumulus Cellsmentioning

confidence: 99%

Heat stress effects on the cumulus cells surrounding the bovine oocyte during maturation: altered matrix metallopeptidase 9 and progesterone production

Rispoli¹,

Payton²,

Gondro³

et al. 2013

Reproduction

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When the effects of heat stress are detrimental during maturation, cumulus cells are intimately associated with the oocyte. To determine the extent to which heat stress affects these cells, in this study, transcriptome profiles of the cumulus that surrounded control and heat-stressed oocytes (41 8C during the first 12 h only and then shifted back to 38.5 8C) during in vitro maturation (IVM) were compared using Affymetrix bovine microarrays. The comparison of cumulus-derived profiles revealed a number of transcripts whose levels were increased (nZ11) or decreased (nZ13) R twofold after heat stress exposure (P!0.01), sufficient to reduce the development of blastocysts by 46.4%. In a separate study, quantitative PCR (qPCR) was used to confirm heat-induced differences in the relative abundances of the transcripts of five different genes (caveolin 1, matrix metallopeptidase 9, FSH receptor, Indian hedgehog homolog, and inducible nitric oxide synthase). Heat stress exposure resulted in O1.7-fold decrease in the protein levels of latent matrix metallopeptidase 9 (proMMP9). Heat-induced reductions in transcript levels were noted at 6 h IVM with reductions in proMMP9 protein levels at 18 h IVM (PZ0.0002). Independent of temperature, proMMP9 levels at 24 h IVM were positively correlated with the development rate of blastocysts (R 2 Z0.36; PZ0.002). The production of progesterone increased during maturation; heat-induced increases were evident by 12 h IVM (PZ0.002). Both MMP9 and progesterone are associated with the developmental competence of the oocyte; thus, it seems plausible for some of the negative consequences of heat stress on the cumulus-oocyte complex to be mediated through heat-induced perturbations occurring in the surrounding cumulus.

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“…[4] showed that concentrations of proMMP9 derived from bovine cumulus-oocyte complexes after 24 hIVM were correlated with the subsequent rate of blastocyst development. Furthermore, levels of proMMP9 were greater in preovulatory follicles from which resulted successful fertilization [7], implantation and pregnancy [8, 9] of human oocytes after IVF treatment.…”

mentioning

confidence: 99%

Developmental consequences of supplementing with matrix metallopeptidase-9 during <i>in vitro</i> maturation of heat-stressed bovine oocytes

Goodwin

Rispoli

Payton

et al. 2016

Journal of Reproduction and Development

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Because latent form of matrix metallopeptidase-9 (proMMP9) levels are positively related to blastocyst development, it was hypothesized that addition during maturation may improve development of heat-stressed oocytes. To test hypothesis, 0, 30 or 300 ng/ml human proMMP9 (hMMP9) was added at 18 h of in vitro maturation (hIVM) to cumulus-oocyte complexes matured at 38.5 or 41.0ºC (first 12 h only). Heat stress decreased 24 hIVM proMMP9 levels only in 0 and 30 ng/ml groups and increased progesterone in 0 and 300 ng/ml hMMP9 groups. Heat stress decreased cleavage and blastocyst development. Independent of maturation temperature, hMMP9 at 18 hIVM decreased blastocyst development. In a second study, cumulus-oocyte complexes were matured for 24 h at 38.5 or 41.0ºC (HS first 12 h only) with 0 or 300 ng/ml hMMP9 added at 12 hIVM. Without hMMP9, heat stress decreased 24 hIVM proMMP9 levels and increased progesterone production. Addition of 300 ng/ml of hMMP9 produced equivalent levels of proMMP9 at 24 hIVM (271 vs. 279 ± 77 for 38.5ºC and 41.0ºC treated oocytes, respectively). Heat stress did not affect ability of oocytes to cleave but reduced blastocyst development. Independent of temperature, hMMP9 decreased cleavage and blastocyst development. In summary, hMMP9 supplementation during IVM did not improve development of heat-stressed oocytes even when it was added for the entire maturation period. At doses tested, hMMP9 appeared detrimental to development when supplemented during the last 12 or 6 h of oocyte maturation.

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Matrix metalloproteinases in serum and the follicular fluid of women treated by in vitro fertilization

Abstract: MMP-9 could be a good predictor of the successful IVF outcome (pregnancy), which was proven for serum as well as follicular MMP-9 levels.

Cited by 19 publications

References 24 publications

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Do follicular fluid gelatinase levels affect fertilization rates and oocyte quality?

Heat stress effects on the cumulus cells surrounding the bovine oocyte during maturation: altered matrix metallopeptidase 9 and progesterone production

Developmental consequences of supplementing with matrix metallopeptidase-9 during <i>in vitro</i> maturation of heat-stressed bovine oocytes

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