Matrix Solid Phase Dispersion for the Extraction of Bisphenol-a From Human Breast Milk Prior to HPLC Analysis

Samanidou, Victoria F.; Frysali, Melani A.; Papadoyannis, Ioannis N.

doi:10.1080/10826076.2012.745133

Cited by 19 publications

(9 citation statements)

References 24 publications

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“…The observed levels of BPA (total forms, i.e., free+deconjugated glucuronides and sulfates forms) were in the range <LOQ-1.16 μg kg −1 with mean and median values of 0.23 and 0.11 μg kg −1 , respectively ( Table 5). These concentrations are consistent with those already reported in literature [20,23]. A typical SRM diagnostic ion chromatogram obtained for a sample quantified at 0.87 μg kg −1 is illustrated in Fig.…”

Section: Recoverysupporting

confidence: 90%

“…Data have been published regarding for instance the determination of bisphenol AF in both serum and urine of rats [9] or BPS, BPF, and BPB in human urine [10][11][12][13]. Several authors have also reported different analytical methods for the determination of BPA in breast milk [14][15][16][17][18][19][20][21][22][23]. Kuruto-Niwa et al [14] and Migeot et al [22] reported data for the determination of BPA in colostrum samples.…”

Section: Introductionmentioning

confidence: 94%

“…This analytical method has been widely used for the determination of BPA in different biological matrices in the framework of various cohort population studies. Samanidou et al [20] and Otaka et al [21] reported analytical methods based on MSPD and alkaline digestion, respectively, for the determination of BPA in breast milk samples. Cariot et al also reported an on-line SPE-ultra high-performance liquid chromatography (UHPLC)-MS/MS method from a 500-μL test sample with a limit of quantification (LOQ) of 0.4 ng mL −1 [18].…”

Section: Introductionmentioning

confidence: 99%

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Determination of bisphenol A and related substitutes/analogues in human breast milk using gas chromatography-tandem mass spectrometry

et al. 2015

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Bisphenol A (BPA) is an industrial chemical widely used in the production of polycarbonate and epoxy resins. Identified as an endocrine-disrupting chemical (EDC), BPA is a matter of existing or ongoing restrictive regulations and then is increasingly being replaced by other analogues used as BPA's substitutes. Human biomonitoring studies focusing on both BPA and emerging related analogues consequently appear as a requirement either for documenting the efficiency of regulatory actions toward BPA and for fuelling incoming risk assessment studies toward BPA's substitutes. In particular, the increasing concern about the late effects consecutive to early exposures naturally identify human breast milk as a target biological matrix of interest for priority exposure assessment focused on critical sub-populations such as pregnant women, fetuses, and/or newborns. In this context, an accurate and sensitive analytical method based on gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) was developed for the quantification of 18 "BPA-like" compounds in breast milk samples at trace levels (<0.05 μg kg(-1)). The method includes a preliminary protein precipitation step followed by two successive solid-phase extraction (SPE) stages. Quantification of the targeted compounds was achieved according to the isotopic dilution method using (13)C12-BPA as internal standard. The method was validated according to current EU guidelines and criteria. Linearity (R (2)) was better than 0.99 for each molecule within the concentration range 0-5 μg kg(-1). The detection and quantification limits ranged from 0.001 to 0.030 μg kg(-1) and from 0.002 to 0.050 μg kg(-1), respectively. The analytical method was successfully applied to the first set of human breast milk samples (n = 30) originating from French women in the Region Pays-de-la-Loire. The measured levels of BPA were found in the

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Section: Recoverysupporting

confidence: 90%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Determination of bisphenol A and related substitutes/analogues in human breast milk using gas chromatography-tandem mass spectrometry

et al. 2015

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“…An alternative strategy for sample pretreatment is matrix solid‐phase dispersion (MSPD), which was first proposed by Barker in 1989. It is successfully applied in the determination of organic contaminants in food matrixes, such as florfenicol in liquid milk, milk powder and bovine muscle , organochlorine and organophosphorus pesticide residues in milk , and bisphenol A in human breast milk .…”

Section: Introductionmentioning

confidence: 99%

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Wang

Zhang

et al. 2018

J of Separation Science

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A novel molecularly imprinted polymer that could selectively recognize tetracyclines in milk powder was synthesized using a metal-organic framework as a support material, tetracycline as template molecule, and 3-aminophenylboronic acid as a functional monomer and a cross-linking agent. The novel molecularly imprinted polymer was characterized by Fourier transform infrared spectrometry, transmission electron microscopy, X-ray diffractometry, thermogravimetric analysis, and N adsorption/desorption measurements. The adsorption isotherms, adsorption kinetics, adsorption thermodynamics, and selective adsorption experiments of the novel molecularly imprinted polymer to tetracycline were also studied. The novel molecularly imprinted polymer was used as dispersant of matrix solid-phase dispersion to extraction tetracyclines. After that, the tetracyclines extracted from milk powder were determined by ultra high performance liquid chromatography with tandem mass spectrometry. Under the optimal conditions, the detection limits of tetracyclines were 0.217-0.318 ng/g. The relative standard deviations of intra- and interday precision ranged from 3.8 to 6.9% and from 2.8 to 7.4%, respectively. In all three concentration levels (1.0, 10, 50 ng/g), the recoveries of tetracyclines ranged from 84.7 to 93.9%. The method was successfully applied to the determination of tetracyclines in milk powder.

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“…Analytical methods for the determination of bisphenol in various food samples [3], as well as in biological fluids and environmental samples [28], have been extensively reviewed. The determination of BPA has also been investigated in more complex matrices such as rat tissues [29], human breast milk [30] resin-based dental restorative materials [31] and saliva [32]. However, to the best of our knowledge, no method has been reported for the determination of bisphenol in bacterial cells.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC-DAD Method for the Simultaneous Extraction and Quantification of Bisphenol-A, 4-Hydroxybenzoic Acid, 4-Hydroxyacetophenone and Hydroquinone in Bacterial Cultures of Lactococcus lactis

2018

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Bisphenol-A, a synthetic organic compound with estrogen mimicking properties, may enter bloodstream through either dermal contact or ingestion. Probiotic bacterial uptake of bisphenol can play a major protective role against its adverse health effects. In this paper, a method for the quantification of BPA in bacterial cells of L. lactis and of BPA and its potential metabolites 4-hydroxybenzoic Acid, 4-hydroxyacetophenone and hydroquinone in the culture medium is described. Extraction of BPA from the cells was performed using methanol-H 2 O/TFA (0.08%) (5:1 v/v) followed by SPE. Culture medium was centrifuged and filtered through a 0.45 µm syringe filter. Analysis was conducted in a Nucleosil column, using a gradient of A (95:5 v/v H 2 O: ACN) and B (5:95 v/v H 2 O: ACN, containing TFA, pH 2), with a flow rate of 0.5 mL/min. Calibration curves (0.5-600 µg/mL) were constructed using 4-n-Octylphenol as internal standard (1 > R 2 > 0.994). Limit of Detection (LOD) and Limit of Quantification (LOQ) values ranged between 0.23 to 4.99 µg/mL and 0.69 to 15.1 µg/mL respectively. A 24 h administration experiment revealed a decline in BPA concentration in the culture media up to 90.27% while the BPA photodegradation levels were low. Our results demonstrate that uptake and possible metabolism of BPA in L. lactis cells facilitates its removal.

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Matrix Solid Phase Dispersion for the Extraction of Bisphenol-a From Human Breast Milk Prior to HPLC Analysis

Cited by 19 publications

References 24 publications

Determination of bisphenol A and related substitutes/analogues in human breast milk using gas chromatography-tandem mass spectrometry

Determination of bisphenol A and related substitutes/analogues in human breast milk using gas chromatography-tandem mass spectrometry

Analysis of tetracyclines from milk powder by molecularly imprinted solid‐phase dispersion based on a metal–organic framework followed by ultra high performance liquid chromatography with tandem mass spectrometry

Development and Validation of an HPLC-DAD Method for the Simultaneous Extraction and Quantification of Bisphenol-A, 4-Hydroxybenzoic Acid, 4-Hydroxyacetophenone and Hydroquinone in Bacterial Cultures of Lactococcus lactis

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