Maturation and conversion into plantlets of somatic embryos derived from needles and cotyledons of 7–56-day-old Picea abies

Ruaud, Jean-Noël

doi:10.1016/0168-9452(93)90208-h

Cited by 16 publications

(15 citation statements)

References 10 publications

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“…Secondary ESM lines were initiated from cotyledons and needles of 7-to 56-day-old somatic plants produced from line 36 by Ruaud (1993). Secondary 541 and 20911 ESM lines were initiated from needles of 2-monthto 3-year-old somatic plants using the same method with slight modi®cations of the initiation medium (half-strength Murashige and Skoog medium supplemented with 3% sucrose, 10 lM Picloram, 5 lM N 6 -benzylaminopurine).…”

Section: Plant Tissue Culturementioning

confidence: 99%

“…Secondary 541 and 20911 ESM lines were initiated from needles of 2-monthto 3-year-old somatic plants using the same method with slight modi®cations of the initiation medium (half-strength Murashige and Skoog medium supplemented with 3% sucrose, 10 lM Picloram, 5 lM N 6 -benzylaminopurine). ESM maintenance and maturation, as well as plant regeneration and growth, were performed as described by Ruaud (1993) except that the abscisic acid concentration of the maturation medium was raised to 40 lM. A scheme for the production of plant material is summarized in Fig.…”

Section: Plant Tissue Culturementioning

confidence: 99%

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Molecular evidence of true-to-type propagation of a 3-year-old Norway spruce through somatic embryogenesis

Harvengt

Trontin

Reymond

et al. 2001

Planta

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Some progress has recently been made in establishing a system enabling somatic embryos to be initiated from old elite trees. We report here the first results demonstrating the molecular conformity of somatic embryos initiated from increasingly old Norway spruce (Picea abies (L.) Karst.), as indicated by an analysis of six nuclear microsatellites that showed an extremely high tendency to mutate during in vitro culture. No allelic difference was detected at these loci among plants regenerated from somatic embryos or between the former and mother plants. Moreover, phenotypical data acquired on the same 3- to 9-year-old plants growing in the field sampled for molecular analyses were totally in accord with the results on molecular conformity.

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Section: Plant Tissue Culturementioning

confidence: 99%

Section: Plant Tissue Culturementioning

confidence: 99%

Molecular evidence of true-to-type propagation of a 3-year-old Norway spruce through somatic embryogenesis

Harvengt

Trontin

Reymond

et al. 2001

Planta

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“…Plants produced by means of somatic embryogenesis are very good donors of explants, because their tissues are physiologically younger than zygotic embryos. The potential for embryogenic tissue initiation by explants decreases with age (Ruaud 1993;Ruaud et al 1992;Harvength et al 2001). Ruaud et al (1992) used as sources of explants some needles of one-year-old somatic and zygotic seedlings of P. abies.…”

Section: Induction Of Embryogenic Tissuementioning

confidence: 99%

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Hazubska-Przybył

Bojarczuk

2011

Acta Soc Bot Pol

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Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens Glauca and P. breweriana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. breweriana) to 23.75% (P. omorika and P. pungens Glauca). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g·l -1 ). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No embryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.

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“…For initiation of embryogenic tissue in spruce, researchers have used immature (Hakman et al 1985) and mature zygotic embryos (Gupta and Durzan 1986;von Arnold 1987;Adams et al 1994;Hazubska and Szczygie³ 2003) or cotyledons and hypocotyls of germinating zygotic or somatic embryos and needles (Lelu et al 1987;Lelu and Bornman 1990;Ruaud et al 1992;Ruaud 1993). Recently, Harvengt et al (2001) indicated that embryogenic callus can be initiated from needles of 3-year-old somatic seedlings.…”

Section: Embryogenic Callus Initiationmentioning

confidence: 99%

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

2011

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Experiments on somatic embryogenesis in selected spruce, fir and larch species were performed to determine if this method of micropropagation enables production of quality seedlings for forest nurseries. High frequencies of embryogenic callus in spruce (23-31%) and fir (29%) were achieved when mature zygotic embryos were used as explants, while in larches (36%) only when megagametophytes (endosperms with immature embryos) were used. The possibility of somatic embryogenesis initiation also from somatic embryos (resulting in secondary and third generation of embryogenic callus) indicate high efficiency of this method of micropropagation. The best results at all stages of somatic embryogenesis (good proliferation of embryogenic callus, high rate of embryo regeneration and their survival) were obtained for Norway spruce, European larch and some hybrid larches.

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Maturation and conversion into plantlets of somatic embryos derived from needles and cotyledons of 7–56-day-old Picea abies

Cited by 16 publications

References 10 publications

Molecular evidence of true-to-type propagation of a 3-year-old Norway spruce through somatic embryogenesis

Molecular evidence of true-to-type propagation of a 3-year-old Norway spruce through somatic embryogenesis

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

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